MIME-Version: 1.0 Content-Type: multipart/related; boundary="----=_NextPart_01C65BB3.9823E280" This document is a Web archive file. If you are seeing this message, this means your browser or editor doesn't support Web archive files. For more information on the Web archive format, go to http://officeupdate.microsoft.com/office/webarchive.htm ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Slide 1 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_document.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1 <body> <p>Deze pagina maakt gebruik van frames die niet door de browser worden ondersteund.</p> </body> ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/preview.wmf Content-Transfer-Encoding: base64 Content-Type: image/x-wmf AQAJAAADjAMAAAcAZwAAAAAABAAAAAMBCAAFAAAACwIAAAAABQAAAAwC7wLpAwMAAAAeAAcAAAD8 AgAAAAAAAAAABAAAAC0BAAAIAAAA+gIFAAAAAAD///8ABAAAAC0BAQAMAAAAQAkhAPAAAAAAAAAA 7wLpA/////8IAAAA+gIAAAAAAAAAAAAABAAAAC0BAgAHAAAA/AIAAP///wAAAAQAAAAtAQMABAAA ACcB//8cAAAA+wLq/wAAjAqMCpABAAAAAAdAABJUaW1lcyBOZXcgUm9tYW4AAAAAAAAAAAAAAAAA AAAAAAQAAAAtAQQABAAAAC4BGAAEAAAAAgEBAAUAAAAJAoCAgAJnAAAAMgohAAkAQAAAAFBoRCBj b3Vyc2UgMjAwNjogTW9sZWN1bGFyIGJpb2xvZ3kgYW5kIGdlbmV0aWNzIG9mIGhlYXJ0IGFuZCBi bG8MAAoAEAAGAAoACwAMAAcACQAKAAYACwALAAsACwAGAAYAEwALAAYACgAKAAsABgALAAcABgAL AAYACwAGAAwACgALAAYACwAKAAsABwAKAAsACgAKAAYABgAKAAkABgALAAcABgAKAAoACwAHAAYA BgAKAAsACwAGAAsABgALAAQAAAAuAQAAHAAAAPsCEAAHAAAAAAC8AgAAAAABAgIiU3lzdGVtAAAA AAAAGAAAAGRpEwACAAAA5AQAAAAAAAAEAAAALQEFAAQAAADwAQQAHAAAAPsC6v8AAIwKjAqQAQAA AAAHQAASVGltZXMgTmV3IFJvbWFuAAAAAAAAAAAAAAAAAAAAAAAEAAAALQEEAAQAAAAuARgABAAA AAIBAQAFAAAACQKAgIACGAAAADIKcQIJAAsAAABvZCB2ZXNzZWxzIHMLAAsABgALAAoACQAJAAoA BgAJAAYABAAAAC4BAAAEAAAALQEFAAQAAADwAQQAHAAAAPsC1P8AAAAAAACQAQAAAAAAQAASVGlt ZXMgTmV3IFJvbWFuAAAAAAAAAAAAAAAAAAAAAAAEAAAALQEEAAQAAAAuARgABAAAAAIBAQAFAAAA CQLq6uoCKwAAADIKzwASARgAAABHZW5lICBleHByZXNzaW9uIGh1bnRpbmcgABQAFgATAAsADAAT ABcAFQAQABMAEgARAA0AFgAWAAwAFgAWABYADAANABYAFQAEAAAALgEAAAQAAAAtAQUABAAAAPAB BAAcAAAA+wLU/wAAAAAAAJABAAAAAABAABJUaW1lcyBOZXcgUm9tYW4AAAAAAAAAAAAAAAAAAAAA AAQAAAAtAQQABAAAAC4BGAAEAAAAAgEBAAUAAAAJAurq6gIJAAAAMgo6AeMBAQAAACazIgAEAAAA LgEAAAQAAAAtAQUABAAAAPABBAAcAAAA+wLU/wAAAAAAAJABAAAAAABAABJUaW1lcyBOZXcgUm9t YW4AAAAAAAAAAAAAAAAAAAAAAAQAAAAtAQQABAAAAC4BGAAEAAAAAgEBAAUAAAAJAurq6gIeAAAA MgqkAXEBDwAAAFByb3RlaW4gZmlzaGluZwAYABAAFQANABMADQAVAAwADgANABIAFQANABYAFgAE AAAALgEAAAQAAAAtAQUABAAAAPABBAADAAAAHgAIAAAA+gIAAAEAAAD///8ABAAAAC0BBAAHAAAA /AIBAAAAAAAAAAQAAAAtAQYACAAAACUDAgAHAaoA4AKqAAQAAAAtAQIABAAAAC0BAwAEAAAA8AEE AAQAAAAnAf//AwAAAB4ACAAAAPoCAAABAAAA////AAQAAAAtAQQABAAAAC0BBgAIAAAAJQMCAAcB tgHgArYBBAAAAC0BAgAEAAAALQEDAAQAAADwAQQABAAAACcB//8cAAAA+wLn/wAAAAAAAJABAAAA AABAABJUaW1lcyBOZXcgUm9tYW4AAAAAAAAAAAAAAAAAAAAAAAQAAAAtAQQABAAAAC4BGAAEAAAA AgEBAAUAAAAJAv///wIeAAAAMgqoAjUADwAAAFZpY3RvciBUaGlqc3NlbgASAAYACwAHAA0ACAAH AA4ADgAGAAcACgAKAAsADQAEAAAALgEAAAQAAAAtAQUABAAAAPABBAAcAAAA+wLn/wAAAAAAAJAB AAAAAABAABJUaW1lcyBOZXcgUm9tYW4AAAAAAAAAAAAAAAAAAAAAAAQAAAAtAQQABAAAAC4BGAAE AAAAAgEBAAUAAAAJAv///wI6AAAAMgrGAjUAIgAAAHd3dy5mZGcudW5pbWFhcy5ubC9hbmdpb2dl bmVzaXNsYWISABIAEgAGAAgADQAMAAYADQANAAYAFAALAAsACgAGAA0ABgAHAAsADQANAAYADQAM AAsADQALAAoABgALAAYACwANAAQAAAAuAQAABAAAAC0BBQAEAAAA8AEEAAMAAAAAAA== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_next_active.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhHgASAKIAAP///8DAwJT//4SEhCnO/ylj/wAAAAAAACH5BAEAAAEALAAAAAAeABIAQAMy GLrc/hAaQYuJOGtYut+ZIY5gaYZASlynUxCw1c50bW8Ttd5LLuy3V4xF83SIvKQymQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_next_disabled.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhHgASAJEAAP///8DAwISEhAAAACH5BAEAAAEALAAAAAAeABIAQAIpjI+pyywPm5yUjYvr hBHoDzbcEy6jEHjlyraudL5H/MZqabvAzsu+XAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_prev_active.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhHgASAKIAAP///8DAwJT//4SEhCnO/ylj/wAAAAAAACH5BAEAAAEALAAAAAAeABIAQAM0 GLrc/pAVIqqJOOu9iv8cZoxkaJ4SAawX2hgTQbRubd84NFUC7e6WHEw2yylgH59xyXQlAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_prev_disabled.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhHgASAJEAAP///8DAwISEhAAAACH5BAEAAAEALAAAAAAeABIAQAItjI+pyzgCYQCt2ovD 2DzXGHni6IDCRCLmmbbuCzcPiKZzSLlrbOw8AAzyhqQCADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_outline_collapse.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhFwASAKIAAP///8DAwISEhDFj/wAAnAAAAAAAAAAAACH5BAEAAAEALAAAAAAXABIAQANG GLrczgTISeWIkrzNVywFAFak5pHkNZkK4b5wDENiOKIrjQND30esAGZX6hgdQyIlmFRmdM7ciejD MJ2q55Q6ZMq+saM4AQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_outline_expand.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhFwASAKIAAP///8DAwISEhDFj/wAAnAAAAAAAAAAAACH5BAEAAAEALAAAAAAXABIAQANK GLrczgSUIim4d8RLnv9LNFVYWXahqQ7shipELM/0DGHUNKrAG2ySyg6gwfiAPJ4PxIQln8dnMipV UaVFzu3J6kauXOCxRqY1zwkAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_space.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhAQABAPcAAAAAAIAAAACAAICAAAAAgIAAgACAgICAgAQEBAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAMDAwP8AAAD/AP//AAAA//8A/wD//////yH5BAEAAPcALAAAAAABAAEA AAgEAO8FBAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_endshow.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252"


De diavoorstelling is beëindigd. Klik om af te sluiten.

------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_navigation_bar.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252"
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_outline_navigation_bar.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252"
3D"Overzicht3D"Overzicht
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_outline_collapsed.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252"

Dia 1

Dia 2

Dia 3

Dia 4

Dia 5

Dia 6

Dia 7

Dia 8

Dia 9

Dia 10

Dia 11

Dia 12

Dia 13

Dia 14

Dia 15

Dia 16

Dia 17

Dia 18

Dia 19

Dia 20

Dia 21

Dia 22

Dia 23

Dia 24

Dia 25

Dia 26

Dia 27

Dia 28

Dia 29

Dia 30

Dia 31

Dia 32

Dia 33

Dia 34

Dia 35

Dia 36

Dia 37

Dia 38

Dia 39

Dia 40

Dia 41

Dia 42

Dia 43

Dia 44

------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_outline_expanded.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252"

Dia 1

Dia 2

Dia 3

Dia 4

Dia 5

Dia 6

Dia 7

Dia 8

Dia 9

Dia 10

Dia 11

Dia 12

Dia 13

Dia 14

Dia 15

Dia 16

Dia 17

Dia 18

Dia 19

Dia 20

Dia 21

Dia 22

Dia 23

Dia 24

Dia 25

Dia 26

Dia 27

Dia 28

Dia 29

Dia 30

Dia 31

Dia 32

Dia 33

Dia 34

Dia 35

Dia 36

Dia 37

Dia 38

Dia 39

Dia 40

Dia 41

Dia 42

Dia 43

Dia 44

------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_blank_notes.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/master03.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii"
PhD course 2006: Molecular biology and genetics of heart and blood vesse= ls
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/master03.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/master02.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii"
<= /div>
<= /div>
<= /div>
‹k= optekst›
<= /div>
<= /div>
<= /div>
‹datum/tijd›
<= /div>
<= /div>
<= /div>
‹v= oettekst›
<= /div>
<= /div>
<= /div>
‹nr.›
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/pres.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0001.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> <= /td> <= /td> <= /td>
Gene  expression hunting
&
Protein fishing
<= /td>
Victor Thijssen
www.fdg.unimaas.nl/angiogenesislab
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0001_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBgQAAAAAAAICAgP///wL/jI+py+0Po5y02ouz3rz7D4biSJbmiabqyrbuC8fyTNf2jef6zvf+ DwwKh8Si8YhMKpfMpvMJjUqn1Kr1is1qt9yu9wsOi8fksvmMTqvX7LbbJFDE3/S6nSTI6/P3vv9f MTcXMAhoeIhIeFCY2OjoNsj4OElZpleJmeklKanp+em0twdKWrokaJqqqtS56vpaIwo7SzvTWoub Cyen2+vrIXr5O0xMwVmMnMxwrNyszOwcTVx4K21NO3qtvc3d7f0NHi4+jiRMfk4Zib7uqM7+bihY DU9fb3+PYo6/n+bO/29pEcCBY/wRPNhFH8KFXOYxfNgkG8SJURxSvHjEIMaN/xklcvxIxCLIkTo0 kjxZ0iPKlTdQsXy5QyTMmSVc0rzpIo5OnDxZ8JHZM6jQoT0UEj26YSdQpEwXKG0K9YLOpVGrWr2K NavWrScYUeWKExrYqnwQfB0Lc6pAtGTlnWX7MhhcsrzmNhVrlyjevEPl8mXq96/euoKF7i188zBi moEXO9YyILLkyZQrW76MObPmzZw7e/4MOrTo0Z5BqXyc2IBa1GEFvmX90N1r2AvNzaaNO7fu3XVM 8v4NPLjw4cRpKC5eO9ht5OuolWWO8Tl0ikanU5duPbZZ7NkJKl/eHdzx8Puqkx/o+/y/9OrLr21/ 0Dz8+fQLEq5v7zt+fOD3c/+T7x87TwV4z4AE1rPagQou+ASADIbD3oPivSfhOfJUSE9/GPpy2obf aOghhxSG+GGHJG4D4om4jKfiM8q1+B+M46QooysG1njNjThKY+KOz4zoYzOyBMmjakTGeGSSSi5J Do1MttPYk7pEKCU2QFY5JXdYbhkilVyuMqSTX/Yh5piIOGhmKl6mScqabH7i5pueoClnneGxaGcl +uVpSo98znnln4AaKWifgRaaCZ2ILprbJWUySsZTj0IahqSUJqoIIZNe+gU1nOqZwKafbuHcqOko aqofP6WKiZasJiLqq7LOymestFqh461kKmKrrlD46SskhwbbxpDE3mHTsX//9KosK8A2ewaz0DKB 6rRixGktGLJlu8ae3KIh7bfijktuuRM4aq4ZlqZrX67sJpSpq++Sata8kcprrxbu5qsvr/xqq+m/ AgOE7cARPWvwr4QmvAnD+iLsMLXVRpzEvhQ36O/FVSSo8RXhdpxPlCBTO+zIJg9T8Mk/hKkyyS1L MfHLRZUscxAp15zDhTjvXEuyPBdh8c8r7yR00UYfnV3MSMdw89I50ey0cVBHbQu+VF+N7NRY5yTy 1ras9bHXEYQttgNWl432tfel/YK3bLe98Ns2kC23sXLDQPfdi5yt9y5E9/3034D7FPDgcBsOt+CI 55Px4ilA7HgHeevddOSB9UBueQY+Z94V53B07TkHlYdOeummHzJ53Y2fLvnqrCel9OvLyB7C6LSr hvntTsWte1K9a57771oL30Dswtt++7bES+D28mY7D330RqQu9ovSFz+888gnz/f13huz9ve4g+59 8MtvLzv6r6u/fvfiv2+2+9Gzfzr9ptt/v/zw778d9WjjTzrl8a957/Mf/w64PgSGSn/nMyDbFKdA Bs5PgXtzoNeC9j0Mlq9wFOxgqHh3QA2WD4IejCAIB+i6EJKwhAg03u9EKD0YTlCCLNRe+MRHQBzW cHMoXCH8VlXDIApxiEQsohGPiMQkKnGJTGyiE5/YrAIAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0001_image001.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh4wACAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAADh AAEAgAAAAP///wINjI+py+0Po5y02otnAQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0073.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Genome
DNA (cook book)
transcription
Transcriptome
RNAs (recipes)
translation
Proteome
Proteins (dish)
#
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0073_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBgQAAAAAAAICAgMz/MwL/jI+py+0Po5y02ouz3rz7D4biSJbmiabqyrbuC8fyTNf2jef6zvf+ DwwKh8Si8YhMKpfMpvMJjUqn1Kr1is1qt9yu9wsOi8fksvmMTqvX7LbbJFDE3/S6nSTI6/P3vv9f MTcXMAhoeIhIeFCY2OjoNsj4OElZpleJmeklKanp+em0twdKWrokaJqqqtS56vpaIwo7SzvTWoub Cyen2+vrIXr5O0xMwVmMnMxwrNyszOwcTVx4K21NO3qtvc3d7f0NHi4+jiRMfk4Zib7uqM7+bihY DU9fb3+PYo6/n+bO/29pEcCBY/wRPNhFH8KFXOYxfNgkG8SJURxSvHjEIMaN/xklcvxIxCLIkTo0 kjxZ0iPKlTdQsXy5QyTMmSVc0rzpIo5OnDxZ8JHZM6jQoT0UEj26YSdQpEwXKG0K9YLOpVGrWr2K NavWrScYUeWKExrYqnwQfB0Lc6pAtGTlnWX7MhhcsrzmNhVrlyjevEPl8mXq96/euoKF7i188zBi moEXO36MUCVkxgbUTk4s8O1lhu40b45cVtHn0aRLm8Zk8rTq1axbu37dQjFs0I1nP6QW2jbF3Loh Gu098Tfwg7g9DxcXjPdxgLKX4xPu/F/q6PymU3++9jpB6Nq7e//S/Pu55MbFRyv/Tnlr7tvR8326 231e+MHl27Uc3zxz+/fVL//0r5p17dkm4EAAnibPRQeyxh84C5om2X8NsjUhchWOVeB+BEYIGoEf PUhaePuAOBp5vl0IForfkFgiRyxuRt+JHu6U32wcdmhjdjLaeOOAOYpWY28qavOifg7KYqQ9SAI3 pDM9QlgbcUVClqF0TWZVpXS6sZdkl14WkeWX3CB5pZiplGnmLFymOaaObCLn5psOxinnilPWiWee IIioJzIm9rnNk4A2E+agwxRqqC+IJtrLmow+CmkCl6AZ6SFPUVrpH5dm+oxod3KqCjWg+inpqMXg ZuqpjqaqJo2sqvpqMpjGSmuttjZ0q6KKzJorGjH2+oqgwIKy6LCTLGnsKjb/Jasms64g62wpvEZL xqrUtkPntZl0pu0nf3a7LbjijkvuetMiNSlGnyK2aZDr7Qrkjq7RuG565x4lqruvWfvcvfjCq++7 rsq7LyH+HnpwuVTUy262Um7Ir5IJG1aZuhP/GzCDwlZ3cU8bczxjvLd1zNOvnJEclsEWD4cyNi0z FuXDP4p8ssKeMFxYsfTgLBiZBM/74cswCw0Lz3/pbG/EjiHNzseXJRgf0TZ35CHNt4Us9dQhDax1 115/DXajRocdBtNkF+Tw2W+YrXbZY7cN90lsx91QzHSrwe3dfmSttw1v9w34doQF7mtyhONd8eF0 8K24T043rgXjkOf09+RM/5hsedkAZ16typybIfnnu3At+iablw6e0qg/EfrqIsztuhHQxp7FsrSb fvsWhueOBey8/w588MC2LrwFmBcfyunIQ/H48kMQ77wEvkfvd/PU+2D79axrH4r13Jf0fUWVh59T 2uQDkff5W9ut1ficbUXet+rTEH/888dSf85Dzz7X7ij537DE/Y99RzNffbyHFtWNpzb8u58MBDCA CEpwAG5xH1b4hA4IRjAAE6SgAo8GwHtocAAc7CAFYYNAB0nQACaMIPQUZMCmbZCELTwh0ARYHRrq 0IQv3E0MM7hBDpawgxzs4QENpEMhKsCFDszBCGc4wSEa8URTjAcRW1jF9//hcEQmFOIEs/ifHy7w iSQcog1vaLWdTSWKHQTjydxYB3OQ0YVwjJ1CQrgvC8LKO9NDWAOdkz4LtbGOcsNjN/IARQnqcWnw GCEL29jEmLDxABskpB27GMQSWpIkm9xEC4eoye988FA1LGEotbPIotVwh2dEZRrHlMklMlGUnUzI DM0ISfHUsiFEZKEZd6mgVD7rk1Lko/LCMcdiGpN0yKwhMNXluXXswYPP5F01XbTFSP7geNqMCTO7 Cc5EZC+c4PsmOVtiznOq0w6jXGdNjulO+sEzng+MJj3vCYnB4fMF8ttn+fzZkmwCtHzpHGgKfmLQ hCp0oQxtqEMfCtGISnQMohStqEUvitGMqqYAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0073_image002.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhbgAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABABm AA0AgwAAAHh3eGdmZp6dnZOSkoaGhqenp7GwsLm5udfW193d3cHBwdDQ0MnIyerq6uPj4wT/EMhJ QUrUKKdG/WAoStdYBctjrk7rgKVEOAWQwhjrtgDjMpPYCvDgDUUtwdBncqiGguRkkQiEoroj8jU0 IhtabwAsGnBHXm0ljT6HHJ6jcbAYCYpZ9Yc9QakKO1wDRQxWAA5KAAEbB24UPAZ1H34WOzIbVRM8 CxyGAIMOhYo3nA81fBJFZ14zAQEcHwpWr28OC0AgqhJeBbIACAqaDgYAM8HFDq6wqsSAwai7q2cJ gXtkW2a4e1xeCU8HT4fSJNXiwua159EjmAhbnw45a9zj2+p8Xjx8x/nSniANWrgDYYTAgwSJ7vmz t07cP3Tm2ERqCJEamDshCnBKJyHAg3ANULs5uBiuHzmSFPW5MQCn4StEEsy4kPTIioBjFaLAlIDA 1iMuRQIAkemEpkl4M1O+CORCiSVLEsCNJDhAga95lgA9dQEvEwCpRrlClUpma4sIADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0073_image003.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhfAAoAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0073_image004.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhfAAZAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0073_image005.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhIQAuAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAwAGQAQ ABAAgQAAAAAAAICAgMz/MwI3hC2ZxygD4Wov2iFYvTaDPQQBh32QOJIZmKosKb1wcrViRFt2B4Zn 69mgWpJD7+IxuhoOxeRQAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0074.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> <= /td> <= /td>
~ 3.000.000.000 base pairs
~ 30.000 genes
~ 15.000 mRNAs
~ 15.000 proteins
protein modifications
protein interactions
<= /td> <= /td> <= /td>
cell type
environment
developmental stage
disease
....
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0074_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBgQAAAAAAAICAgAECAwL/jI+py+0Po5y02ouz3rz7D4biSJbmiabqyrbuC8fyTNf2jef6zvf+ DwwKh8Si8YhMKpfMpvMJjUqn1Kr1is1qt9yu9wsOi8fksvmMTqvX7LbbJFDE3/S6nSTI6/P3vv9f MTcXMAhoeIhIeFCY2OjoNsj4OElZpleJmeklKanp+em0twdKWrokaJqqqtS56vpaIwo7SzvTWoub Cyen2+vrIXr5O0xMwVmMnMxwrNyszOwcTVx4K21NO3qtvc3d7f0NHi4+jiRMfk4Zib7uqM7+bihY DU9fb3+PYo6/n+bO/29pEcCBY/wRPNhFH8KFXOYxfNgkG8SJURxSvHjEIMaN/xklcvxIxCLIkTo0 kjxZ0iPKlTdQsXy5QyTMmSVc0rzpIo5OnDxZ8JHZM6jQoT0UEj26YSdQpEwXKG0K9YLOpVGrWr2K NavWrScYUeWKExrYqnwQfB0Lc6pAtGTlnWX7MhhcsrzmNhVrlyjevEPl8mXq96/euoKF7i188zBi moEXO36MUCVkxgbUTk4s8O1lhu40b45cVtHn0aRLm8Zk8rTq1axbu37dQjFs0I1nP6QW2jbF3Loh Gu098Tfwg7g9DxcXjPdxgLKX4xPu/F/q6PymU3++9jpB6Nq7e//S/Pu55MbFRytvnhv3TejT83hq Rrn7J/AttZ+fw3L8+/if8f/vb8N6Cf0HoC3ZkSFfgUlYx56C9FVGYDkROijDhEYkSOGFAm6BYYZD WLggiB7mc2BBIo4Ih2RhdIjiDydq2GI5hK34YowhkGfihja2pIYsO37YI4s/4lAfgkIOGaAiNQax JJIaqChGk05iwOCU00BpZTE2ZXmNlFx+CWaYoHgpZh3JlTlMlWjOouaasOjoZpz9tSlnKj6SWSca eOYJyZF8lkLnn58EKqgmhBaaCZxZ+InooHvWGd6ij8qJo4mNroAleJPGeagVjF6aVIlRbupmp1V8 CuqTqF6xaqqPtOrqBJeQGiCtYj5lqy25gonrGbDGCsFOhOwaw6/APkCNr8T/cumVsseKgNt+ij4b 7LSsZkqtVMLat2yYxlb0bbYNdPsCueI+eG660pirLitKtltJkfA2gm0yZ3poqpb41rsuvpWRw65d AdsxMFg+hlMwVwnfsfBW1trr77/IRSxawz3uW9s2FmO18Rsdz+vDxyC/N3K8Gc4KMIW9Tuzgygir XLHIep5slrMLEpmxw+FCsXOxPP4l76juJgn0u/sNHYtg+tmHNA0yR9czDLQ+PZ6oNDZtIND8ahF1 uT/ztaWRWFfoGNUddJ3T13kdLPbNRIP98KkfT6200Uy7nXTR27Yt49trD6vn3GrXfTTeTiud84Bx H+63wFZrunjWeYMcueSM/4P9OOSGWy5wNmY/ObbPRa8heOOdf47g4HPlO43qcLGeputsyTMO3SWf IjtaYX9jO+Z785677r970/vtfU9ufEbBJ+/i8gajXUvxjkv8sumvZ66NQ+VpT7ho1S+zM3eoIwc9 NuCTh376iY+vbivqv//+Mt2zD0Y1lUNoP+Jsd8M9B/1jLjH6GaN0yFuMAGVFwMsZ8IASKB+mnPcs B6pAequbUf0SyLnV3UtomyPb6BgYCAx6EDEgRGDopKY/CcKog6IDYD9E2EK4ldCELESh3rzHN+VZ b3Z2y+GFIKiVpfmwCBSs4AtP6LUbzjACKiTRDnXXw1Hdz4YF1GATQ7K1kv8AMStLHGDHishD6hnp i1vkWBYXhcS0fbAfaYxN2WpXxqtskHhxtArsfgFG5gEhj3oMWR37qMAMGjFlTxxL0Pj3x6gcUj2J hMoZm8FHg60jkgrDXpcaeZdHQqyQksQhOLqIGZapTJPIACVLTIlGjBESZmIEHs0C+EmM7U9jFFsl IP+Aylue7Ue55NmI5sjIV3qSjsLspRSMSZIp9gKZuqRSLVtJy5MBM5qjvKIqmPmRO/oCmxzR5jIp xs1mMtGa4iwnc8hpTqSFM51OsSQ7o+TOd9JonfKsZx+UaU9fQjOf8IQlP3M0y38mRKAELeiQ6MlP fBq0IwuNzzAbOiCIFkRyopqj6ECHZ1FPRTGjGkUnRz/qit2BdAqLHCnPMGrSlF7yoSoNxUZbGgqU wnSmpfQoTVNQ0pv+UKY6JSLgegrU2CUAoeysVFB/eNSI7DOpQOIpU13k1KdKdapUrapVr4rVrGp1 q1ztqle/CtawinWsUCkAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0074_image006.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh6QAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABADb ABEAgwAAAHh3eGdmZp6dnYaGhpOSkrm5ubGwsKenp9fW18HBwd3d3dDQ0MnIyePj4+rq6gT/EMhJ q70465nS/lQHjmRpWuIJBoojsa71zPSs3luKh97u/7kesOKwAYqPi1EySA6f0KiUJHBOZdYlRQuw Xr/gsKopniy5knN5zW5PBEX22Wt2Ery1+yxgcAQWDwYvfQILQgNFDAEvgAdOfw+OLy0UBQkPCwWT LgoPDgQXMACinZ8YiA+KXUY0lYAJiwACDTMJmnwOhTUMEgwzoACWmJoAl62iEgWvsRbKD7AUqKpp WY+NNa0UnQUIqjSatAdHMwMDC8kPAQGYEguL6+3vTkgTdwgAvppI93fnFvT7APS7UCDdOn9z6rkD YMAfoAB3XCAptwDBMwoLBEiwh+8BsSX0/wQubHiBwEh/wQyyo1Yn3qhsd1CsZPkS4QONHLClaVDh Ac+eVmih65FQiZOiFYxlQ5ogxoEYCWxSw9lxABNxEoSmJIrHSVMJTy98BRBWglKkrH6uMmPhDq+W NLmgeSWo3bO6NNfGrYaBFV8sgPOiYWIsr8CVQvTq1eJ3w+DHf+kqLvkL7mI6eyzQCjRhM160R/8G RrolHVDLmSn8SaDHcMcDBBSUhss4NDOjt6nlVrzEMxdLExgUcIDSSJWfXC7xhEMhthfnli99RC76 NG86Zn3KisFUO3MAgPZWiNiAajGPOw0bUb5drPcY55fDJ+1cCwEH9wSwPix71T1awHDRBLwN/ZlW SBqLHJjXHR5YFGB1s113wYAz9AeIJhatRKEDBapkGhqXvFXPRQ5KUEQAbxmxYX8VrDgBhQ+wWJSB gGAzg0at1LCKIwtYtcpMYBWh1gPmLNRFkbHomMwrH+WYTWk2/ogdWULWU4RsHDLzVIwvYhJAVHoA CUATwLjyDDFjPrOIkluyaEGbFWw5pJM2ELmAkSYM5saeYvzB55+uASqoFAf4OGgbeh6qqApFwLeo GEo+KukJTZU5KQkRAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0074_image007.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCQACAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAJ AAEAgAAAAICAgAIDjH8FADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0074_image008.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCQACAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAAAI AAEAgAAAAICAgAIDjG8FADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> =
Aim:
Selection of genes selectively expressed in a
particular cell type or under specific conditions.
cell type
cell function
developmental stage
environment
disease
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBgQAAAAAAAICAgP///wL/jI+py+0Po5y02ouz3rz7D4biSJbmiabqyrbuC8fyTNf2jef6zvf+ DwwKh8Si8YhMKpfMpvMJjUqn1Kr1is1qt9yu9wsOi8fksvmMTqvX7LbbJFDE3/S6nSTI6/P3vv9f MTcXMAhoeIhIeFCY2OjoNsj4OElZpleJmeklKanp+em0twdKWrokaJqqqtS56vpaIwo7SzvTWoub Cyen2+vrIXr5O0xMwVmMnMxwrNyszOwcTVx4K21NO3qtvc3d7f0NHi4+jiRMfk4Zib7uqM7+bihY DU9fb3+PYo6/n+bO/29pEcCBY/wRPNhFH8KFXOYxfNgkG8SJURxSvHjEIMaN/xklcvxIxCLIkTo0 kjxZ0iPKlTdQsXy5QyTMmSVc0rzpIo5OnDxZ8JHZM6jQoT0UEj26YSdQpEwXKG0K9YLOpVGrWr2K NavWrScYUeWKExrYqnwQfB0Lc6pAtGTlnWX7MhhcsrzmNhVrlyjevEPl8mXq96/euoKF7i188zBi moEXO36MUCVkxgbUTk4s8O1lhu40b45cVtHn0aRLm8Zk8rTq1axbu37dQjFs0I1nP6QW2jbF3Loh Gu098Tfwg7g9DxcXjPdxgLKX4xPu/F/q6PymU3++9jpB6Nq7e//S/Pu55MbFRytvnhv39Oiesrfn /j09y/Lr22ey/v436/r3Z//vT448AMKD3oC+SGagNwV2MECDDj4IYYQSTkhhhRZeiGGGGm7IYYce fghiiCKOSGKJHJb03wsDJJjEijjIIoOLLBohow0LblDjjEPkSEN4JfCoIxBAykAeDEMG2cORMNyo gZJI6uDkC0xiEOWTN1TpkyJTUoCllTR0qUJ8RnoZBJgpINiCmWSqmAN/Kai5ZpptorkCnHHW2WZl X96ZZFEz2MknCoBiMWigP7ZRqKEjJJrPmIriwGgIyTn6qA2RfuCmoJVemaeeMW5qaUpbcglqDZdK cWqpVHYqWgypqmrBq0llM+oDssI6wa0Z1FqBrrhC4CsG+Wn6K5svpuhCsMX/MqBsIMjKuSy0LT3L QrPRImCtMcole221iHaL57EJ8NpAtt2aG0GRxoJLbEvDnoButPE+kCm87L7JKrm23tuujdSGy++h c+q7b8ACrzFvsQkv89OeBpOwMC9irvtwCBGPq2WrlFYMwsVmibattxxbLK6nro5Msr8ff4ryBx7/ FzLALXPw8iLv2jszgylpTHHOGdQsB8Hl+kyznywT/fO3SCfNBtCVOm3yn0szPe1OR0/da55Wn4x1 rAMLPXTXWZcM9gJQj3Fkg+HkqLYIUMPI9Stmnk1HlbK+HfXGj0BowIpq+z1h3w4G8DfhgLfty9+B I3630XFT4nffhBs+uYsy/0YueeWCT44E35Q7UTjnnLfd+It0qkA3E5gPbvnoB2DueuSwH7E6FLJn rnnrbrPqcCW1Z/675sDnLjrto6d+we2iK1+66WWbrUmNfEtoOOCCP1g94jQWj3ys1LPuecf5Sg3W 5Zc7grenz+MutgRvT9pz+wVPG6r87ittP7D45+9A9+Tz3z/n8Sx+AEwA0J6yPmwVMID+mhi3Fsis qlUmga+DYATpl7cHWtCAourdBit4rK3p7YN4EyEBLVhCCoLwgytUg//WlL4BSouFxYvF6fBFQ/bF IoMapCHyVFhDEn4NiC8k0/tiJjMWllCGIsvhEomYQx32iBBADOIGf/g/H/8OEYk4c+L4rqbE/YWR bFkco7uEkcAieimGHjTjGdsoRDKCMY4YhGIU08fFfrkxDWq0Eh7h6DvdYc9rEdCeKUh3PTthSXtP LGMmpGdFBkLvgquApJrSFjYp9siEPfyD4kJXveyxLnuVk53iKDe45RkSUoNcZRHA57nDmbKU1vsk 7lKpSTP0kQetK5wgiVe7Wi5vc710XZ90p4RfBjN2qBxmM4nHPTF6UnLFZObhjrdMagpvlJEMleV2 OclifnN1oYPd7cZpzFyWAZxQ0uY2tWnOW7oTmPLspqmi2aLhBQ+Z1aSnMyE5MED6IUKorJAoEylL 7BGUdLjcgflaSAQLIXT/ooub3jjPJ8fH7XGKPGyiFjM6wit20JEibVoU7Smlf6HupEe8oQnYqaMY 2mSGG0XRCSHovzxCjKXSLCkGVfpSnjoPbjSlo8qqCNMZYXGOPrWRS7v40Z+SFIUjZSpVQ8jEJBp1 h5wsalO5ilShlmyqONVaVld6R7Pa0YtDFWhZx0rWBco0rgVs6VqjelS3ytVxIb3qGZ+607SC9KZ7 NalgESZWFyZWZXqtq1rpCsBGWrUYSeWCK8fWpK81do33hOtk0WfOhOLylAqt7EQ/p7pSivKhn7Te qgbbSUz40p3nhCfwTPvOJ8z2neK87Wv/etdH0taaxjzf7Iz3Td3qk5np/1QeIekX1k9Uc5nUtW0y 8Znafw63usnjK2EbAb6CVlS8trzu5tRZplNi83W1LC2c7ApZPmEUh1L9bKAYaq2z3exgeN1hR7X6 VY6CrK9vBe5+xXdYxm6Wf7gNaoL5uNiYELiwQz2wyyJsC6DqcasCDi6HNxldtiL2wQLWcGBF7NTa ANivKQ5xfzsc3/zN1b6R/aJGP2wLnUK1prrEcI5dzOMY1MvBKPbvWen74gz/F61FtiGQcbxOHxNJ x0ROMpFMXOUgL2ktaZTykuAX2wArubNNPkODAXTmE1vZyBN2bFXbXGPT1a/MU/SwmG0YY/st9cZ3 nvKT+7zlrnpUyykV9ICKC9zAP7MYwiRWsqIRzdUjbxjQX5b0jqFMhjTr5495lp9k+bxoPBPRRKQu talPjepUq3rVrG71iU5qDQvDGgsOnLUYam1rMNAn17y+iI96XQV1AXsTwzbDTItNbEMjm9bKXraz nw3taEt72tSutrWvje1sa3vb3O62t78NbvEUAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image009.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhZwEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABABU AREAgwAAAHh3eGdmZp6dnYaGhpOSkqenp7m5ubGwsNfW18HBwd3d3cnIydDQ0OPj4+rq6gT/EMhJ q7046807Tom3BYojngCIasKyvrClxvRFmu98DcnzJAbBo0YsonQ0h88oCxkNB6b045zClMNctZJY DCQBxtJKLtfGRKE5ETC73yK0sYGjiOH4vEVOG2TJdXqCcHxEDwgWAX+DjGSFLwJKZQMKjZaOi0UP AhsDSg1tNwAKDw4EE54PoBipqwA+PgSwAAEHDgELD1G0ti1OrW1guQhZuIfFJRMFPQsFE6KkphdL pAtts69L0KWnKbBDotvSE9FfGAzOO5/B37Latsa7tQ6+EsDPw8X5YMkSyw/NniWL1q2CADE/nInr dtBHgnTZABDz0UBCg1jGiPHDkZHPtyEF/x4EUOQCiwEAslwACDkS4AWWJMk9KGDAFSxnYhBhGTDA BUyXEqzRyiJUkQQsEmSdvJjOJEqgFXycDKkSzRKnKSegQYoVKikCBrBFxfBT5aiZNdn5wHkIwM6e K0XGDBqKaN2jY5QCYIr3wcmsFnIFkGWiq0rBhLVmCbtlAaeiWZACgJxpqEgBPb5JsJols1gKnuUw i1oMqA9OEkL/ecAgamvSEu7ErcJ52uostSNuvk1hTG7dql6+lvERNNTdYExvmqBa6/DNz5EDkB2S Nm8ZVXFf7yJd994H5gYgehUd+PAHBRV3Vy/9Ue/KSYO/Zy/nUa4Euyb/yA/8uvdHuQXoX/992rHn Fm6BkGPOHvA9VdF87S1i334T3MefbwP6ZwEPGBoIAIf+AVaFhRCS+EAlsGUTjIEYrmibixO4k56A 78EYmw/5IZRfh939BmF/9GUI4W9+GCDLeBUkWOMFMv7I4242TofjBDoGyaKG+CTgTo/6aOnjRQgQ gOKNuhioYy4LStdDa5FwmRprALRJHJuBNFCAA2bpJsRrosEpZ4ykUCDmdjNBd6VtVkZ4aKLeTeeA A0hSEMAWXPhZ5515jrHnemvGmeCgggaqZqHkLVpBLopKh+p/ixDGAGqAajjopIeg5kcWt/qAYm65 nrjDNyhidoosY0olZTdy9FpsGy1sxqz/WXn9AEBYBfm4aC7OhOXSb0oE8OCTaBwA6wUHnMQKsBII +1Sxfh27nrJaPetsnNBmIUsI1JpaoyIisQolv2pV+uC8zbYn74cXOSTuBAgowecsmknkcAYNw7nb EBH7QIwXF+dZ8XPgLSDUKz2NHHEB90GkWcSKLaEZAUpU4oAC/LqMjR9sZPMRxth85ECak41rwcct 66zdxubA4vHEWpXMjtMXZ4FyQi3zXIgf1nThjs1LYD3pAqew/CF6vUFtNMkiR3kJBu6t7TYHqcCi pBRtvz0ILnabUXfefNuzYMFl7N33GwgAPbghDR7ON6QTHGA43YkrXoYSc0v+gtiW8/0PCtV6f5b5 GuPEEAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image010.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLQAbAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image011.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOwALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgA3 AAkAgAAAAP///wJLhI8XYZwPo5zJ0SutYgus/nkO+HGmuZGhx27t2LwsJ8OWHXe5XO/03FMARTog LEj78ZQxnqhRgpZQU93tKiQ+MdwsRdMNI4oZMKIAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image012.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhDAALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAAL AAUAgAAAAP///wINjANgqe2MkJIpsvdWLQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image013.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhJgALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgAl AAcAgAAAAP///wIwRH6gyxacmnyLStjsVOghl0Qf51SdQVIeiapsWL1oK7u0Z7t6zVsrGMKpfEOh 6FAAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image014.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLQAPAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image015.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLQAbAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image016.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLQAbAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image017.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLQAbAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image018.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLQAbAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALBwAFAAJ AAQAgQAAAAAAAJn//wECAwILhIMGGOKMnEwLmgIAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image019.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhJgAaAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image020.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhIwAZAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image021.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLQALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAq AAcAgAAAAP///wI0hI+pFrHYQHx0uSpZvtK1/zEc12mm52Wblpbq216xOqez2cnsvq42PoKwUCWQ EQU6TCLHAgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image022.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhDAALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAwAL AAUAgAAAAP///wINjANgqe2MkJIpsvdWLQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0005_image023.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhKAALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAm AAcAgAAAAP///wIvhI+pixHgIJs0Peli3kfnfl0SZoTkY4ojOaLl67rsW0btfbIyvd7fBkQJVTbP pgAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0006.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> <= /td> <= /td> <= /td> <= /td> <= /td> <= /td> <= /td> <= /td>
Targeted (known / small number)
• Northern Blotting
• Dot blotting
• Real-time quantitative RT-PCR
• RNAse protection assay (RPA)
• RNA in situ hybridisation (mRNA ISH)
Random (unknown / large number)
• Expression arrays
• Differential hybridization
• Suppression subtractive hybridization (SSH)
• Differential display (DD)
• Serial analysis of gene expression (SAGE)=
• cDNA Amplified fragment length polymorphism (AFL= P)
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_master03_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBgQAAAAAAAICAgAECAwL/jI+py+0Po5y02ouz3rz7D4biSJbmiabqyrbuC8fyTNf2jef6zvf+ DwwKh8Si8YhMKpfMpvMJjUqn1Kr1is1qt9yu9wsOi8fksvmMTqvX7LbbJFDE3/S6nSTI6/P3vv9f MTcXMAhoeIhIeFCY2OjoNsj4OElZpleJmeklKanp+em0twdKWrokaJqqqtS56vpaIwo7SzvTWoub Cyen2+vrIXr5O0xMwVmMnMxwrNyszOwcTVx4K21NO3qtvc3d7f0NHi4+jiRMfk4Zib7uqM7+bihY DU9fb3+PYo6/n+bO/29pEcCBY/wRPNhFH8KFXOYxfNgkG8SJURxSvHjEIMaN/xklcvxIxCLIkTo0 kjxZ0iPKlTdQsXy5QyTMmSVc0rzpIo5OnDxZ8JHZM6jQoT0UEj26YSdQpEwXKG0K9YLOpVGrWr2K NavWrScYUeWKExrYqnwQfB0Lc6pAtGTlnWX7MhhcsrzmNhVrlyjevEPl8mXq96/euoKF7i188zBi moEXO36MUCVkxgbUTk4s8O1lhu40b45cVtHn0aRLm8Zk8rTq1axbu37dQjFs0I1nP6QW2jbF3Loh Gu098Tfwg7g9DxcXjPdxgLKX4xPu/F/q6PymU3++9jpB6Nq7e//S/Pu55MbFRytvnhv39Oiesrfn /j09y/Lr22ey/v436/r3Z//vT448AMKD3oC+SGagNwUmeOB/DCqI4IPaLCghLuFViAx5GG5D4Yaz dOjhKvGFaM2IJJ6X34nD8KfiihG2OE1lMHI4Y4023hgOiDg2ktyOv7DoIyxABvlKikQeKd+QSJoi i2hLfvikizpG6YeSVHpi5ZWZZKklasp1CSZ1F4Y5iYZklvLimZpwqSYibLYZj4NwYvnlnHaedsmU d5LxlJ57htHnn6iJVqegbpplaDoJ+JloQ2Yx2ugWaUYaz06UpgPppY5qymmnnkqQ6adVmCiqHaSW SsekqK7x5qqAqurqGTbF2keotLIC660F6Vprobya0eqvWXQmbD89Fisrssr/LstsszTk6Syfilga LRiBVmstobZimwM13Iph5LdTnCouFuSWawV96K5bT7DsGtHku+bKKG9C9Zqb671J5KtvOdP2O+q/ AI9LyLYDv2DwwfnUpjArcjYM8YoPR9yRMAlT/MHFGNfk68ZCuOvxsxOHHISAJJ9cJL0oF3HuykVR 63LMMs+8Wrg0ywDyzfmMrHMsPPf8bMdADw3Jz0QjzPDRMRCrdExNp/R01P0QJnVOx1adk8pYi7w1 DPF2HRvYQWvcdcti4yHw2Suoq7YKZINtdtsgxC03MDbXrcHbcBuNNwdf9z3CrIB3NTjHfxfeQc6I L85446Y6LgLdkEcg+eQP1vBreQN6l8135g4c7rkxWoeeN+kYgG46BJuXLXTqTnXuenaCx77M1bRr fnvuugOxutK2747o6MAvCvvtdw+PPNXCD29m8pUd77rik0sPOfXVt+589otin7v1jXvPOPjhc6+9 9tCnLj7iTGffvPO9lw//9/EvQn7s9esOc/z3Gz9/we83XTnaBdB+BeufAdfyP9+lDX4/OaABZ8e+ BZavgQ7s3/lMN8DoSTCC+6sg+pSHvPYlL4FPg6D7Nsi+/HlwhSxsoQtfCMMYynCGNKyhDW+Iwxwu qQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0006_image024.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhZwEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABABU AREAgwAAAHh3eGdmZp6dnYaGhpOSkqenp7m5ubGwsNfW18HBwd3d3cnIydDQ0OPj4+rq6gT/EMhJ q7046807Tom3BYojngCIasKyvrClxvRFmu98DcnzJAbBo0YsonQ0h88oCxkNB6b045zClMNctZJY DCQBxtJKLtfGRKE5ETC73yK0sYGjiOH4vEVOG2TJdXqCcHxEDwgWAX+DjGSFLwJKZQMKjZaOi0UP AhsDSg1tNwAKDw4EE54PoBipqwA+PgSwAAEHDgELD1G0ti1OrW1guQhZuIfFJRMFPQsFE6KkphdL pAtts69L0KWnKbBDotvSE9FfGAzOO5/B37Latsa7tQ6+EsDPw8X5YMkSyw/NniWL1q2CADE/nInr dtBHgnTZABDz0UBCg1jGiPHDkZHPtyEF/x4EUOQCiwEAslwACDkS4AWWJMk9KGDAFSxnYhBhGTDA BUyXEqzRyiJUkQQsEmSdvJjOJEqgFXycDKkSzRKnKSegQYoVKikCBrBFxfBT5aiZNdn5wHkIwM6e K0XGDBqKaN2jY5QCYIr3wcmsFnIFkGWiq0rBhLVmCbtlAaeiWZACgJxpqEgBPb5JsJols1gKnuUw i1oMqA9OEkL/ecAgamvSEu7ErcJ52uostSNuvk1hTG7dql6+lvERNNTdYExvmqBa6/DNz5EDkB2S Nm8ZVXFf7yJd994H5gYgehUd+PAHBRV3Vy/9Ue/KSYO/Zy/nUa4Euyb/yA/8uvdHuQXoX/992rHn Fm6BkGPOHvA9VdF87S1i334T3MefbwP6ZwEPGBoIAIf+AVaFhRCS+EAlsGUTjIEYrmibixO4k56A 78EYmw/5IZRfh939BmF/9GUI4W9+GCDLeBUkWOMFMv7I4242TofjBDoGyaKG+CTgTo/6aOnjRQgQ gOKNuhioYy4LStdDa5FwmRprALRJHJuBNFCAA2bpJsRrosEpZ4ykUCDmdjNBd6VtVkZ4aKLeTeeA A0hSEMAWXPhZ5515jrHnemvGmeCgggaqZqHkLVpBLopKh+p/ixDGAGqAajjopIeg5kcWt/qAYm65 nrjDNyhidoosY0olZTdy9FpsGy1sxqz/WXn9AEBYBfm4aC7OhOXSb0oE8OCTaBwA6wUHnMQKsBII +1Sxfh27nrJaPetsnNBmIUsI1JpaoyIisQolv2pV+uC8zbYn74cXOSTuBAgowecsmknkcAYNw7nb EBH7QIwXF+dZ8XPgLSDUKz2NHHEB90GkWcSKLaEZAUpU4oAC/LqMjR9sZPMRxth85ECak41rwcct 66zdxubA4vHEWpXMjtMXZ4FyQi3zXIgf1nThjs1LYD3pAqew/CF6vUFtNMkiR3kJBu6t7TYHqcCi pBRtvz0ILnabUXfefNuzYMFl7N33GwgAPbghDR7ON6QTHGA43YkrXoYSc0v+gtiW8/0PCtV6f5b5 GuPEEAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
RNA isolation
RNA isolation
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBggAAAAAAAACZmYCAgJn/mf///wECAwECAwP/GLrc/jDKSau9OOvNu/9gKI5kaZ5oqq5s675w LM90bd94ru987//AoHBILBqPyKRyyWw6n9CodEqtWq/YrHbL7Xq/4LB4TC6bz+i0es1uu9/wuFw1 cNTn+LweNej7+3uBgoMZd3cBh4SKi4yIC4mNkZJyh5CTl5hpfpmcnWKWlp6io1J/f6SoqU+Gqq2u TqGvsrM5prS3uDexuby9dHa+wcIippvDx8gYoMnMzRDLztHO0NLVyIm71tq4p9ve3+Dh4uPk5ebn TMbo65iV7O+S7vDziobZ9Pj5+vss6vz/beQBHKjpEcGDZwQiXBjGH8OHYO5BnBilG8WLVSRi3LhE /yHHjx0tghyJRCPJkz48+ijAsqXLlzBjypxJs6bNl0Bu6tzJsydMhraEFMgydCWWoghZDUFqhekO p1OgJl1K9IfUKFf5KRUKgoDXr18vgB0LIqsNsxbGgk2r1mtZoI5M1kA7oa3aunbXdqA7g28ErwIC Cxbs9i+BwYgLc/BLD5BcGowd5L0LYTLZxVY5AEbMWQCByp05f8aMMkfkBpb1Sk4dlnSP0wsOh04M evbg0Rpgs3OYWTNr3Kh/A8+gm0Vx2bYDD4+dnLDrg3WiU/XN2nD15087IJ+9nMH20N0tFD8n/XHf D5YppP4wXkX7r6IVG+7cGvvA6ObPd6Uslr+H9v8pAKiAfxW0FYKApR2onl39GVhgXhQgaAJak7HF 4IMOSiBhgv9JcJ11FXq4XgQbkmDWd4mFNyCKt+HFnYoBlMjhBlkJB2J6lf1GYm8PsChabcmp6CNt EMjoDST5vVCjjT3q2KSTDxipYI/NKQdkkFfaFp6U2lDz2o0hPjnialA6wCV7WXKXJnhr/lgkUEjm BCaEOX5IJo5vGtVmi381J+SQzuU5FSAKJNkChXjOqZqii0bJ45PxwbgiivVZF6mGcBZK6KMiNrrg ZQ1WiqmeeHn6qagYouoonCJ96UGijEpawZkdvkrnqQTmBlcDhhpnK6x33kojpxhsBh6MxtIn647/ C3nJw3hMignsBbTupVmVnu3J57DNAkNsqGN6F62upGYA6LaoYbvsqs0G9a2F4YpbJnHvqqeutuhi UK00xQQB7bzyTkttvfZi2aeW67ILnbflbiBwcMJy6yp136kKcYoJC7owr3KGkCuuIuwrMXqmlipf rVNxTPATIpPb1EP9dnxFy/TOPONZPuWs884uWcXzz0DbBDNvZtCshtG74nf0K0h3W6gjaTR9htQb Q90rFVSXkTVB6lwdFdM3c7L1GGOH/bIrZWtl0Bppg9G22VK87YXccDeBEyp3161I3qT0rHcFzpLN Uist/W1BMZtqPTjhdM+DTeJag224Ml5X0fjk/5pAjvnmJhDN+eccPF456JsjPjrpkweO+uoTeM76 68+sDfvsEahE++1Q54777ohozvvvwN+uevCfm07866cfP6PrynNeXvPIxwU960pPb/31YTOPfd22 b693997DbU/4pCdPPkStni+++s7Lzr746b+/vPypM0x/gsbfz6H5+lfd/83P+9/+pCdA/GmvgBgB HwITGL8FbmQrDiwN/yJIwQpa8II9mCAGxYG4DU5EgR68j/tCODQNkvCEKOQCCFOYj6CYkIXTgOEH fSfDfaywhu+4IQ7XocMdouOAPgyiECtivyHupoNGhEcDk/jDETKRh058InmiKEVzALGKWMwiDf82 8UItyuJ5XfRiK8AoRnJIp3dlNCMDwphGUsSpjeB4HBzDscQ5WsMxdhwHDfN4Rz768Y+AVFsgu0TA QcbwjIZsRh0T6YseMnIW7nrkMSAoSWawsZKEiCQmG7lJRe6xk7RwJCjduLZLjpISSDxlKFXJyla6 8pX34yIsvyg9U85SDWS8pSrO+EldZgIbvnTFFYMZj0IScxQBPCYy0ajMZjpQlM4MhCajeQlKUrMT trzmF6apzUYsspuDSCY4IyHOcTKieuZsRzrJGbN1ZpKK7oyn8OApzzm4sJ7SxKc3e6nPN0Czn2b4 J0DJML6BGhR01jyoP42pUDjgsaEQjahEJ5r/hGFSdJv0vOgnMqrRiHC0o9vkJ0hHyrWPklQLqTxp Q0qp0oK0NKDZfKlMczG8mVohfzbdQkJz2oWY8rQG3PzpTYXaEJESdRUMPWpGkqrUKaCzqVfwKVRh UM6pEhGRVi2FRbNaBKlytR8m/SoRgirWij6trFTwKlpHkNK1liSsbo2rXOd6QbXS9QNVvasQ8qpX IHyzrzuwK2A3INDBUvWvhrXBThP7A8EyVgJkfawuJDtWo1I2BoW9LB9Yqlkc4LSzQAWtaEerRtJu sZ2mpSpcU1uCzLIWr5Z9rWzTWsTZpuCztr3tVnOL19XytgOu/e3hfCtcwsa2uMitrGNJG9zkmdaO uM4FHHSjS4HdUve6nj0udqs73e0ybLHePVxbw1sI8pr3vBhFrwesq15mtne9untvIZabWvqKlr3t 5at8n4vV/XJXu/4NcHnjK2D+ErjAD3goghcMAvAyWL8MRqN9I/xb/KoXwgvGMIKfSuEOX6CmBcZt hCc8WgdnmKkdVrCHV8ziFrv4xTCOsYxnTOMa2/jGOM6xjjuQAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image025.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhiwAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABACC ABEAgwAAAHh3eGdmZp6dnZOSkoaGhrGwsLm5uaenp8HBwdfW19DQ0MnIyd3d3ePj4+rq6gT/EMhJ q700JIe7/57GgZ2ikJcTUE/boukLz5NrLwblyPRk9pKHQ5B5rGaCB0sJnAV4hsdh+UlOrM1K68Tq DZhBcJbEAwipnu9EPa4xHosucodug8p4sUVAB/D1WUoKDwk1FASDDQQSAQdDDSY2YQAJDw1HAAM7 CyuNjwoilEIFHTwEhHWIlosAgy6uL6EAoZUOpBO1A0F+O7pmEwUPCAALD4s7DwMDDWZiLcOnzACn AU/MyMoNyMPB0hc2W0Q1TMHDxaw8PMgS2wDduA8FCJKzLYtgbxKnXC3izXUyYMnoN64gBh4FFKgw mG/aA35g8hj8N8lMHDBfVETcWLEjRYplxzymAzTRz0ODIztKVAmmzxkxUfp8HOisJkojWmx6zMmz YsqZOoGuERYsh55KYAahYyAyaMAHTPk0LdlTwruKSoMwFTrxpwQGDhzkMINJwiBgJ+fdKrMjQByu X1wU+sjVwpMH4lSxohjshFp2RuK0feuVxwF/YfSM0NfgIboW3r6AavaCnoEdW11Ik0RvCTgHDG5R ZkKgsYK9klekHs2a3mdfe+zInk0bhCYbi2vr3s1bgrIr3noLH55F7IQDsIkrXw5ClWMAEQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image026.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh4ABGAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADc AEUAhAAAADPM/3Bub4KBgpKRkp+fn6urq7a2ttLS0sDAwMrJydva2v9QUP9mAPj4+OLi4vHx8erq 6v///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/oASMZGmeaKqubEuK biy38GzfeCrVee/LvJ+wFBwahbuj0lhc5prOKA0qreqsPSp2m9x6UdovWEwGdMvfMHqkXg/Pbmsb PY9ntYG8fs/v+/+AgX0rdWSFdjdwiE6HaYtSio9HjV6UkiyRl0iaL5yTeIKhoqOBhJ5mp2+WqSar cqw/mbAzrlW1rLKzMbdRvKe5AKTCw6GYv7pPvseeypzAyKbM0InNzsvTQFoNDQDc3d3c3uHh4N/f 2+bj6eXq4uXR1tjZ8rTX9JjVmvlv9y7P/WzsAQSjjdw5g+3YjUh4cB06hurg6RtIaJ8ki7EoqvgH EKMPj3Y49gP5RKMOLQwY/wBQuXKlSpYvX46Q2TJly5o0Y97UqVPiJZQ5Xe4cahOmUKNFZxINanMj yThPE5kkOHWMtKpEQBHbyjWAsatY2URdM7ZeWLFnO4ENKxLHDgkGzCAwk8SAg1drl7yNK2HuWwB2 bXnse9cM3xEJ4NoSqBdBYcUwEsft5WuHggcKJu8YwAbCABgLEkBYnPfO5cxiOZvxDFo0JGUSBEg4 AENCaDYEHMB4sKMAJMZZZNNmc9tM7t29KU9CVRuAZ1SJR9wl8OB36SzMAz4XER3A9OqMKr8d33s8 gAgAeCdIvK8sECbkxxcwj149+/BpA1432Zae+1359SfPf/4ESKAj+2kkIN82B351VnwQRijhhBRW aGGEv1yo4YYcdthgJcDlh9WHG4loIolXmBhgiCoqyGKLA6FoFYwjvkjjPTKekOONsZD3k488dmTe RUMGOdJf+iBp5JE7itXkktRkCGWMNk5p5ZVLZrKgjpY8iSWAM+r43pds/VUDD2fqRxeEdK3ZXG0i mHdmnHSSOVF2zQX0Znb65RmnnnXy+SeaXtrZBZJzvplEmqg0+qege67ZppN23qlmo5jyiamfenY6 6aNBcFrpI0MueoapgaJ6KJx0ttoqWqtuOWovapE264iyZlPoIyEAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image027.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhaAALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAMAAQBg AAkAgAAAAP///wJ4TIBgyXoNo5x0KYrjSxv2DIbI54kj54zWdXLPZsTptcBvU1vxwetO7ZKxgLQh 6sRDKntFZSuJAt6gSVi02bupVFSjbBo8Qp1U4ldb7HY+Y3DTexSzyMYnbphjboUrnT+fM7OSwrfz A/iiJ2QSQsL4CBnZOChZKVIAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image028.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSwBOAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAABF AEsAggAAAAAAADPMzICAgP9mAP///wECAwECAwP/CLoMMTDKSau9NLTdHv5gKGlc6YloOpam6qYk 2700GMvLWe/TjTu84OqXqwiOyKQApWxWfDhdpKlkUpFPYpFyxYq6x6wWyAVbu2KtFAJefs2ZMXnS Pl/TxPWg/kbHx3p8IYJDakZwg4iFeYd+iY6LP4GKH4QRUDKTkJWUEJgsmnd9oj1yoVR2qH+GZZsY lp6mjaQgsAOfLa20nK63srqqj7uxgLPBtZ2+xcBOo8eXv3TJFra4HKfNws/ErNK91MnWG9hVztmR UcbnvMPK3RK24L3iMxQEBEf3+fgo+P4C/wjgkVRBH0B8B91oW8eNEbNyCyGiy6ROIrtt7hx6ayev /h09BuSSpGKYkeBDkeYsNjS5EWNHjB+3tCT5kmRMBSG9RERZatlMlReqRYM3rSLPiaCEKL05R+kO pnqcqoAqlQfVqjWuYn2hdetUUwHCih1LtuzYAmbTqg0rpy2LAm7jysUBd67duwDq4t3bVi/fvz/8 Ah7MQTDhwwoMIyaseDHfApAhO2YcufFku5EvU7aseS7nznI/gx59WDLgzKZliN6wuvDbBnAtV56d N/YC2rEzJ86dt3bd1LR9/xbeu3jt47YT906ufLnt4cahJ39+uzl158pRMxjO3Xr14tMl65W+vHn5 8duzqwf/vXv025V3o56fW/du38J5y58dn3YCADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image029.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVQA4AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABS ADQAgAAAAP///wKSjI+py+0PEZi02ouz3rzT4IXiSF5giabqdK7uu7XwTMv0vdr4Tur83/EBhxgh 8chCKk3LZtK5NEJ30umtap1hs68tN/cdesOoMbl35pnToTU7+L7Ga3NtHea+M/Vgvirv9xRYAhhY 6HfIF7HI2OjI0JSoJ3lHWWc5hxmn+cbJ5kmmMNgmMUpqYHqaKoK66gG6UQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image030.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhdQALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAMAAQBs AAkAgAAAAP///wKPTICJpnrAYJtUwufwrbH1mnwOqHHg6VnpaFLl+S4SY9SHTecXHdo7rpJFaqGi ivj7DTsPXtNy68lmVMzzutE0o1Uh9ejFdp/d7wZaRM64UXIYjbzxwGX2Dj0Vn8nySf8d1AY3YjU1 KMamcyTHiON4lrfwuMQImfPnc0fEZBUTgwIaKjraQuryaZqqaqo2WgAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image031.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOAA4AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA1 ADUAgQAAAAAAAP///wECAwJ/jI+py80Co5y02lsD3rxT7YXiN5YjaKYbqrakC0ts7M60at9mrp99 /Wvxgp0hcXXcJUvGZcYpakJlU4+0KrhWtVMu1OsEL8VJ8tFMRAdzDjWH7Qi12Zn4ud4oY997ZP/i 1hOoM3hTSHMYkwizCPRn0Sj0CDn5VDkRiTO3yclZAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image032.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOAA4AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA1 ADUAggAAAAAAADPMzP9mAP///wECAwECAwECAwP/GLrc/jBGQqu9OOuNFf9geHliaY7BqZrk6nLt K6NzbcWVoO+8UPbADI4C7P2KO2EKg0yKmjplBup7QqXMq7WJvVCP3M7Sqw19xdMy6EzLhs3qypDA Xscp8/pHjx9b+BuAeXeBhINvdohyfjmEGoKMRI5pin2USGCYaG6acJUEh52JoouXRZmnm2Sfj4aR dJOcqW2rpByQpkFbtqCvgK2fcwMDOsPFxCXEygLLA10WxszE0lWevKGzo9mlsrrW25bdRrvgvbnj 3943vrG15djqe67nPKjx4e73hcHsrPROtP60E8jPhsE5BlUgTMjiFcOGD2UsjAhiIkUYEjJq3Pgg AQEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0007_image033.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLwAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAgAt AAYAgAAAAP///wIahB2ny+0f4pt0xluzvrz7D4YYwGnmlpzqUgAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0037.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
<= /td>
spot sequence(s) of
interest on filters
RNA isolation
RNA isolation
reverse transcribe and label
hybridize
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0037_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAADPM/3Bub4CAgIKBgpKRkp+fn5n/mZn//6urq7a2ttLS0sDAwMrJydva2sz/M/9Q UP9mAPj4+OLi4vHx8erq6v///wECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIEJo2LkJGSQASVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbFq5QLSo0aNIkypdyrSp 06NYnkqdSrUqUpLOtFyIs3UonK4giW0B64bsFLNr0IYdy/WK2jRvKYrVigOB3bt3X+DdiyOuE78u 9uINLNhuX6ymfDYBvKKw4MaOB9dgvIRyisiSUWDOO/kkJsVMLJ/YnNkEac4zRCNRTeI06hKuEXQG GoV1a9eaY8+uYltEbNmjdaf2jIktDeGmkcvoTYT579y4h49sRN34DOWwo0vnXUN7cNK7P1YHXfnG ZhbgbTAfsj5AehXvt4sHRb583ccwCudoH4S/b7754XeDf7Tth55jeiHYAv9mLBDoA2AMLhgZYfqt 4GCB6sEX33cRXrZhCRfy4NdpGp5XYocnhIihfNmRCN2HI2BHgoo6xCXjfy5yaGKKiJFQ3xE23uie d7cROaNbL8I4pJE45ghijz5G5aGTLSr5HAo0GqjjjlVayaQIWcpTnEdIJolil2cWyaUJYQ445Zpo KrjlhFgiNuaPRUAIZ3J0nihnnV69+aeZAgpaIaBhOYRnnhS+JiGACZamQpsZHijpo45ialiDxOnE nQ178nloeGeZ1ydkgy4HJZmBHqdkjK+2QCmpAcb6payrirAoe93dKiSurcZwpaihArtWCbsKsd6w asZqYZkyMNtssc9ilRX/tNF+KS0Ms9LgnJDbutBtOt1ksayvVMYwrqqu2pruC+uSw86nvZ5qKKTe YpttmsQWyuJG81LhoL+a1qhvvZvWmrCb02FzcFoPw9WpSQK3FSwb8a6YolUcd+yxUW59LPLITk1M SMaBoNxwYslCbIzKxyYmCMx+0MyRQi1L/LLGNgiUMxo28xE0z28MrYfRRGN8DNITCVXz0km/wvQd U0ddBlTAYG01J1rzAvLWLAS8R1HFkA122N38bIbZwhB1dtg+jvn0zm+7IDcgVdcty916980N334H 3kncagtuddqFG5602Io3fgLFjkfusCmSVz554pZj6HTmkUPO+eegS854/+h9I05645ifDpTnApPs +utf8wb77CPbSbm5FtPbRt7KjGddWREDbWfqPfC+g/HGa6k6p18Ff0byxrBeavMXuxwz8cfnXnHR UGJvMPW6K70y4NujIMD56Kev/vrst+/+++tP6vzaLHmvPPDVqwF9MZ7mr//8V8sVXcBXvt1173cm gJ8CF8hA+MnPf8KbTv/CJz4K/m9lXdgfwyyos5ixioMdLGAFxZM2KaWggShMoQKrBcLnCRB3BJwe /jCIwBHK0IANq84AuQdBF+bwdiZEgQQkEAAiFrGIRDRiEpOIxCMecYhOXGIUmyhFJTbxgS2knwTJ J0IbSkGDtMLI5m6Iw/8sBnCLXPyi9shovfkAMYgngKIV5SiCKlKxjnd8IhP1yMc5GjEFyIshDQF4 RjOSAYwsQWS+eqjF5bFwhl284EvsF8YRRCACAcBkJjOJSU12spMiAOUmL7nJUYryk6VEJSqxGElL hpKTpkwlLD05y1eSkpa4PGUtWUnCaxnykIQcgyJ1MUY1CrI2x7xIMZF5QhU685nneyQbJZkoSh4m mU8YprocyTwe/lIM2qzFMqHQpqIoIAAXYAA63aaACSgrmKkhyjnTuc6ttLM/1vKGNf8lIga4E53z FEEDLnBOIASyP/4EU0ADMNCCPuiFcFTPBRxAAQcUlCgFAFMFCtCVBzT/oAL4ZCS8JlrRi14go+jc aEc/atBOceFCFxjABRbQlQt4FEwGmEBXKECUA7RUpPCSKU3BdFN05nSnPf1BOGkxzmxmbys1DcBG 0cnQrrjTABT46Tc5BVUwSTWjWxmoCK6a1YdicJ+VXM5SDgAyCwSApw0YaJYO2pe1tvWtF4ir24p3 wB1Ccpoh7OVL12jMv3KTTYRlpmHFOLmthoGuZeylL1sZ2MJG9mbS+yLtNuuxkHH2s1Pp68ygRtpb fBBvpS0bH5pKtdQGY6k8yOzRXJu1mcC2kW1b7WcKcdtC5ta0vhtEb4FJt5LIDK0ZpK3X9qDDNFKt a7q4ymtXG6WUxW4X/9DFLnORNTO2Zc27yzXtKZA7lr2+1rzTZa5s84De8xZXvW/8w3CFqdySkPew swXEffGLh/nyNx3+/W85AlyD/Qq4tdTV4YGl5ofgLngVBKbBBB88igjPgLUUhoSFZTDZDItiw4OL r4e5JggDj7iNt5jwiSUBYnGsWBUtFodzX5yIGL8Aw6EBrY6f4tkd+zgpc+sDziLqRcUuFsFCLiE8 wbnkxwaZul6wMWQvy153SLnJWIAAj4Qmj+FC4MtgfhJQtQDmL4t5bF02Q5nNfKQxZ3nNWh6Bjbup WwXDEAUJyLOe98znPvv5z4AO9J7hzGavMlLQiE60ogFN6DajWcjHrf/hFwgdZ0M7lgqUdnR+Eywz v4ah0ZoGrBdAbekqN5hyM3ZqGeB8Zsp2gdWhRjKkT9vDRdv61oiGtZznh+te+zrPui51f0ucalWP IdO7drMVkC1sWUPazlieArOpquxlB3vOvOS0icWl5jW3WtSvvvaj9Uufwabg1+i29bSnbIJ0uzvX 4t60lbtd5m9bVgzBprapya0rSY863+wGA6n1Pey9rfcvqx54wP8d74J3RMQtpGMf8ejHKUrcjk+k +B6TOHCCgzsA7w75nxUu70Fs21j4Bni0pe3tWNMB2zI++GLoHWaXk5PmhW72HGAe85Oj/NMqrzam Se7wjkC7hxe3+Mb/84jxpSf96R1f+AhETnU9r3vfRof4x8kc9EsPveU6lwPPb1xuf4cb7GE39rEb XoexX9jsZ695sr3O8nrbHJt1VvHNU3BLW9pSl32PJS7/LsvAdzLq88ul4Amv+Mb7ffGxvLqz+a31 ewu8667eguTbro/eIl7oX5d72o986n57+vJo97jlJ010zmekHj5H1AmqTvvPZ5H2VN/8zg8Re26T wfaZ5zrbxY4Imc/895jfuvDtPnoUm5zWdJ+UURRAT7Ld8wbAV75azVl9dv6zBrpP7MMrb+TsBYD6 AKV2Q3GQ/NVrCf0EVX/8bdB+b2ZEV70HpA6o71Z9Q1WlIuBRIMV+/5Vmb+VnA/xnaf/HUQHIUtgn es1XWffgIBOAUv5nVDolAjx1AT4VUtFnAhU4d1B1VBqYVFolfg+Xf/qXA+4UVefXVWIVAGT1TqCH Ai1oaPBXVWMVAFjlgShIbkf3gSRAUw5QT/JENm4FV3Llg9rXAkRohASFhHilV2GCbW4nYUEYfL7V hExGfP1wZTWYXD84D2AohKdXZPhQhlpIXPaXCMYXGmMIh3jnQ6wAfa3zY3h4XWeRh3wYY1dYYHZY M3+oBO1FCoP4dqZnCOAFY4dIg5DwhmPTiA8iiUz4NovIYoUIYZKAY/KViTXmiTA2CUqGiRpGiYwi imSighkEioKYXv+joIpfwIrsZYpAUgrFhgiXaAe5aAu0WA27+BWySAu9GGLVxYhtN4yEuAmmAwu/ uDbImIzKmIjRZWHBGF6veF7hRBbN6Iqa0GHTWFMa5DbbyI3dCA3ZtYfVqFqveIuxcI7kNI7q2BCd tjTuuBj1WF+omIU05oXXyI77KIHR+I9F14/kJ5DURJCwaJB8lRCQqJBg8Iw3kJAOKSIFEYgTuYW2 qHcX2YWvMBcbGUG2IJEfyS3iVC4jSV/iZJEnCXcrORIQ2ZKjBZMw8ZI+IJIyuWW74GA3SWQpqY87 uXfAoJGd1zH4yFQR0VnxGAycaGVE+Vv8I5Rp2JReQ5ND4JEDgZT/2hUNNmmIUsmLz+CNX9iVwkgP RymWzEgNDdkOWMmMVIkES6mWZpkKbZkEQwYQaxmK1rCM/7AUcqYUUjOXSrCVjJhdfomXP6lZ5whk pZCOh2kEigki0vVhgFkFgqkJjzkjkWmZkwkFo+gPl9mX94iLm8mZKpkPhQmaoclbo9kMpRmVHEOK 85CW6nCXh8CYjfmOrymaqxkUjRWWuama/KCXnhmXfQCP5CKbAEacz2Wc7fCWs6mcXKGHA+Gcyfmb GMMUctGaEwidBomc5kCbtzmU1vmR3jlg3PmP1Pmd57mP6Wme43mR7UkO4DmR5SmfSyFXpxme/KAU cdWf+Ume/thl8EjRnwT6mfCpnfaQFATqn5m5kXW5lwO6oPgpnfTZmb5pFBI6ocx5YpW5mApaoAaq n64ZoRpKoSKaoOvZnQgxnxXaoZvAot3popL5ng5akCNaFTIZoHBJo+S5oilKY/UZDjAqkDpplz+6 YkUKoTyqkNQho5q5pCcapfpglVLKEElapQixW1i6pVw6DNLYpQRxpWDqD1o6pmZ6plgQpGiaDWK6 pvfQpm4am2UXp3Rap0YwOna6Dhaap+rgpHzKe1/6p+4Ap4IqLz5ZqIiaqIq6qIzaqI76qJAaqZI6 qZRaqZZ6qZiaqZq6qZzaqZ76qT4QAgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0037_image034.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhwwAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAC3 ABEAgwAAAHh3eGdmZpOSkp6dnYaGhrm5uaenp7GwsMHBwdfW19DQ0N3d3cnIyePj4+rq6gT/EMhJ q70yJHc1x2ClKGGJBYtJjSqrvvC0CNNj24tBx5VjX74Hz+IanhiDWDG0NDonBEdAYttFpSaB8PLj bkPaSfhZEjgO5LTadHDQuhmHu0T4VuB3O6YO1a9lJH+CgwAMKXgACA8GYD4YiDV+FWZblIQTAQ8I l5xkipl6WgwTAwoPSBKmNxY2AQyLFHilp0kAqja3Qh4AuwkPDgUUvg4EIAYPwbwbAMNJvgzJu8oc w9HLFwINuLUBBm4MCt3fJNnbijgSCzbJBYuQXe1o6rWQVA9J2pv2E/EA8/sAAQQRaANNu1ESfBU4 sIrIlyBoBpwyeErCQIIPKCK8aOFVgHYf/4IQIMBAJMlCDz7+AsAwkAQGOwBoUeenizYJEgPVA9Al E0KeX24CyBkQTxc4SLc8SOEl1pekkaIWlWQLYdKYVSYosLpFXTEABPRFQiRqasA8UoGmPfrUDtu0 jnh+YOW27Vq7ZoHiIaAqrdoKfOFVtEUXkaIEeXcinQsVYGPHeMFmbCcW7d3Lef/e2OJKQbvIfzOA +yxDUwHEefAEkKP1HpUGZ51meIA6tCl6sEMbVQq6gZzKFdRhjfy4ONVXmUOjDAiyQcwaqqzImSKh HQmGyXygsOCjmO/h/B5cR2YxZQrtTN8G1GHiWDLdxOP7dRoAFPXd9O23Zkqh3bHNCwA31IcrCtQi WTgWCOALbcPBMQCBBtaBoIRTbLaZXrzdQEwHrmHo4YUgrhJiPXUwEMBWwdzA1WCSmYhiH+9NwBB1 ndSoQhSbzRUcfzb2KNoFCvDo45AUjCTGT5OcQWSPCHxV5BxLRimQWAY4acFqVkq5hg864mSillHO Ip6BGAggJJhkKACMBQvQGAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0037_image035.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhGgA4AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAX ADUAggAAAAAAALvg4/9mAP///wECAwECAwECAwODGLrcviTKSauqON/M5e7cB2LiSJWmF6Tkyp7u q4boTAp4DN943lq9ns42CQSFliHBeFQunUxkUmOsPTtOouy6jVhN31EYNMZmwWdxmrw2d71t2pvb tTaopIFeD1UG9nt9FoCBUyeEA4KDgIowhT9UZ2Vyb5N4lXGXdZmQm3OWLQ+iDwkAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0037_image036.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhGgA4AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAX ADUAggAAAAAAALvg4/9mAP///wECAwECAwECAwN5GLrcriTKSSsJNluse+SeBoYbKZrZiErqerlU Oy3nFgi4/FY37stA36/EE+aIMaMAuFEybTmd7jPdwT5XVha7rYa8HnBHXMuSU1vr9Yxci2jozWA+ kMrp82cMn2+z+HV+H4B6e3R2KXCCK2w8aY0xj5Jdk2aPD5iYCQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0037_image037.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhGgA4AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAX ADYAgQAAAAAAAICAgP///wJ5jI+pN+0Po4m0zordzXhz6n1QKGpBCZ7oqK6m+5COQHftQ+eCdc96 Lek1fkAWhbgLHompyo8He0UHslJVdP1kOdtM1zalClFf6LTcPI+t60ZbzGOkWYl5TGE31ZXBvTEl xwcIphaGJghz+BeleGf4xgXpJWmzYLlQAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0037_image038.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSgAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAMAAgBG AAYAgAAAAP///wIehBGny+0PowpU2osj3bz7D4biKGbmmW3oyj5JC6MFADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0036.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
RNA isolation
RNA isolation
PCR with specific primers and measure fluorescen= ce
SYBR green
fluorescent probe
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0036_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhQAAAAAAADPM/2ZmM3Bub3h3eGdmZoCAgIKBgpKRkp+fn56dnZOSkoaGhpnMAJn/maurq7a2 trm5uaenp7GwsNLS0sDAwMrJydva2t3d3dDQ0NfW18nIycHBwcz/M8z/Zv9QUP9mAP+ZAPj4+OLi 4vHx8erq6uPj4////wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwEC AwECAwECAwECAwECAwECAwECAwECAwECAwb/wIBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+Cw eEwum8/otHrNbrvf8Lh8Tq/b7/i8fs/v+/+AgYKDhIWGh4iJiouMjY6PkJGSk5SVlpeYmZqbnJ2e n6ChoqOkpaanqKmqYgdGrauwsbJgB7W2tbO5urtRr68Bv7zCw8TAQ8HFycqqv8jLz9ChttHU1ZrO ztba24q3t9zg4Ye+4uXmhtnn6utx3uzv8G/p8fT1rK72+fpa3tP7/wChYAtIsCCSgQYTGkSosCHA YPMcSoT3baLFixgzatzIsaPHj4T8gRwJrRnJk8pMolwpzFdEljBjypxJRiTNm6VU4twp7RjP/5+f dAIdmskm0aOYXiJdmqgi06eNlEKdOkgo1atVnWLdCkgq1692rIIdG1Yr2bNzyKFde8cr27dh1J5C Qbeu3bt48+rdy7ev37t3/goeTLhwXnutEqNCEYmxHceOINPD5TaT5EaX5WRWtBnumM5UHlh5IHoL aDeno5AeXTpLanNGSb1eQrq27dVMbuu+MltNbyS6bzcJbps3YmOVMf0+Qlx4kubOqSw/M50I9OLP r+OeUt2a4uSXugvRXhs4+e1SxH8Ofb51kfZV1EdLDD48+/Pm8UsPfJ98fv/ceZaGevAxV2CAj/Wn 3X/XxScgGvIByGBzVsgXBoESGrjgfg+aYf9hg7lBZ1yCVYBIm4gVHkdEfZP8Vl5+2bn3HnozyqiE hWC4SKN1Ng7xooYxPoHjMgyJkpqJSiAZI3FLDOnFkSgmGaWUFN6IGDIsNkalkjxuOCGTSTjZBWj6 fRncllMWIWYx9Pkk25Jc+lhmjRmqyZ+GdcqZp55eGrEmMbgAE+ibeO7ZXo8BHBrmnUYoWmichyL6 J6DulELmnHTGmeiBfjKaqaaONoopEZO2hA+hZkb3KJipqtopiaueCWeVrf54RKm8FBkKlLSiuVuI rC4Ka6vDpVlrkyquaCkUO+qZ3ZLPCunpidACB2e0TeCaa6WoKvjrib0iWEeEmnYZrLiTcTv/CoZ9 itpuFNqm6O25n9IrbT26gsLuu+NxCu+0T4Rar71OxJtLvp+IJ7C5/BYMsBML89kwEwbPgrAn+4Lq LxQVo8vsxv3u6fCVZu3KWrmbTpztwxCLHDLKyHZYxofGxmqrx5pdUbO7BN+Lb2xGajksIx3HQs6g 6wo9LiRFw2JSlkpHxjIiTa+iEtSPGKb11lwD9ljXYIftVz4iYV1M1SbLPAza+qq99jlsT+ZmPHFj 7LYwdXeS992G7L2J33wPArhlO10szuDKFd6P2bzgFY7jM0GEtDlec1P5TZOrY1c5m9ME9Dp1cU4X T59Tjnh4p4sjOeNvU654P4HHToXhstd+/1DmtueuhFi6914E774HD3zwvpdO/PHII0978ncvzjrz Xz0P/VrGT8/3d9b3jn32ubfJ/fcYiy3++J2PS/75YZONe9BMT31I6p4Mn3DUc8BvWrLSE01/zlmr uH7b7RvaIuwHivwNcH9xIKBr1Pe/+QVwaf3DFyxOI4AKWvCCGMygBjfIwQ5iMGYQlJoEhWBAziAQ DgrEQgqLUjL2RTCEmElWKlY4Ihjqb4TK6pYRPMjDHvqwgyCkAw0d9DPY6fCGNjwgDmd4wjcMkUNL nEsTUfPAeGzPFBT8oRa36MEg1q+KFEHOYqbYhifijB0tdKEIk2hCHJYwEWY8IwrB+A51Hf+RCCEI QQD0uMc+9jGPftQjIPkoSEEKwZB/RGQhA7lHPgqLjXD0HxPpmEBKWnGMlnRiJsEnhDimx31946SV kjDIRjKylIk8pSFRuUhCmtKVgBwlJKlGkDcWwpP/EmAbi5hGvZGRDbj02SVJiMkXCnGTsJnbspIA AiE0EwTPdGYAoDlNaVKzmdXE5jOjmU1ubrOa2aSY+7Q5TW+Ws5vg/CY5r2nNaLITndh8ZLpsSUtk AtOe3wumML9ozDASU4r49E1AwVEpegpOCVxMqEIt6EX+rXGYqtDnyGb5PgZOsp8OjaEbi/kkukAg ACioAEhHB4ERiIGAuKrLR0M6UsaU9KT/+OPokypgUpCuVAgWQMFHcwTKCtG0kzcNQE53+gWJOkJ+ dnMNCi5AggvslC4I6GQJEOAYDFigBBfqabaW2tSnoiCqIJ1qVa+aVZJdVIUEQEEEHIMCq3YyASNw DAnoogCe6pI7aV1rJ90KUrjKla52FeWt7scYtgZgqiAVqmNMmgASBPaYCyxsJw8bVcbkVAiMdWxR ZfjPpClVLwrYnAkCMFcL5LRUKP1sXkIbutGW9rSb5WUvOWFUcd61nvM0aCgHCiHebgOpvvSth4Tr HWXecZeQfag/jQFQjFbSuWhsYMLQR12uBaa62CWMYDWRWtlNQ7cA6W7gsAfefYj3emIs/68+zts8 5kr3JOxtr3FlEl+1YWkn9ZWZ5PCrVbjMliX5dRtl+Hvb5qk3HwFGL04SvF2JMLjBDXnwgw5s3v76 V4wLtvBbrkgTCXvmvyjx8IU722ENs8WO9DUx9Uhc4gLL9yYi3jCISRLjEw+lxid+L3xVjBbg0pjH Z7laiynaIecNObmxo3CFXQxhheC4yUsmMpQN8mSyfJcnVQYLeQks5QtzeCZZjp57lUyPMIt5vjEx s5Z1/GMmC+jLKXbzhzEMYyBbWVBYtvOUy6xnLaMZJmrmCorT3Oczk5nPci4yl5E83hmPJNBbcfSj Cy1oOh+Zn961NJgpHWk8LxrT3ft0Rv8bPeiVQBorPp50ovccZUYrmLl1XnWO/XFouJUPhbd+8YJD V79cG7jW7+B1r2ubkFR/ZHTmI/ZC/gxgTqOahMB+irJxkoFqW/va2M62trfN7W57+9vgDre4x03u cmP7CuZOt7rXze52u3vd6GBxFTJwEnpTwd4jwXdVNH3ves/b3+hQDBb0/RGCQ8HgHUF4JxS+EYYz weEZgXgmJH4RiiPB4hPBeCU07hCOE8HjCgE5Har3BJEbxOQmL0jK4WBsJaw8ICgHeEiYfXCZS+Hl AMF5G1qeBJ3vI+Yk8TkbSO4EoecD6PkmhdGX8IEnNN0JT2e6GKIuB6SDZOlo4PnFv2D/gq57/QMm +IDYw+71sncd7GJHu9nLnvaxr93sbX873NMud7bTve5mN4PVt4D3vvv974APvOADr/eAl7rkZmg7 1Y+geCUofvFFeLzjG58Eyc9h7x7B+hmE/G8yPB7yQ/h85S3PeNJHXvSlp3zVO590RkBN80T4vNhT b/rQy572qo896k+/ezhgPuGuf30Zes/7to8+98VPOxJuj/vZX571V1d64o0/eeUf3/rLR37yQW97 6q++30GP9++sgPW1o33sYJf7+dVe97j33f14h3/77/73woO/C4PPv/73z/++2z8ki0N+aMB9jNcF BJgFB7gGv8cRsFcGcoFuNhcFDfgO/xO4eVlQgeuwgA1neGy2BBioDhqoER9IC10wgsA0bRIIfVmD gjVneFpggmuAbJgQgnDEgohXCHA2BTDoGzZYdCoYGT3YBDvIBTl4cy1yGWa3AFagAXhAg1STGUm4 hHcwhFvgPQN3hEYwWgGwAFq4BF3oB074PpuhhVzYBF/IB1RYhVyQhh4ChUNgAlyohF0nAQsQhwGw AW/HAWVHh1vodW8Qhn0zhm9oh3NYh3B4h3m4h0rIhV3nBmyIBUWYgpIgg2/4hidQAPZmAhOgiY0Y AKP1iZ4oBJ+4iQFwAqboez+IGYIoipeYiZu4iVoIiqAYipxYiqfoiPEmcBA4iW7Yh/9+SIsmoIei 6ImkGIu0GAAUcIh/mIpEs4qM2ImfGIzGWIvTGIrJqIS4iINEJ4RYSARJuIkS4Il7eALhuAFbKAFe pwFvlwHKmI062I3eWHYLAI7i6HUSQI53eI7puI7tyAaPeAX584+f0TQn0InuaIS8iAYFeYZqIJBV oHVH4JBFdTlhYIfLeH9A6GtgYJFtIJFTcHgtuILCZgmASAgaKQkeKQUPKICNMZIbx4yc4ZKUkJIP eYG8GIQPB5MxqQk0KRBGpJM1yJNA+YRCiYM0d4O/9AglKRE92ZERGJLRNwpNWQxL2XHSF34YWXDj sIZPiZRROXO6yJKt945YCZawJpb/WdB/armWbEl4ZVCVTNCWcjmXcvl/WdGBHtiVPliW+8aVfCmJ Y9mXZzmUFUeYFjGVTQCSXjkMA1AHcBly4sc6ODcAlBkHldmYZkCZjYmZTqCZX/CY8gALiCkQNnkF mlmZWMCZUnCaqAkGIvCarPmasjmbsykErKmaL2iYIxcADtCbvvmbwBmcwjmcDrAEtHmcyCmbdikI irmXVXCbuBkFpxkA0bkE0FmdXACdnXmbJaibaWEG2+iY4rcFK6edVGCeT4CeYHCd2FkE6lmaZMkH zkNP4UkHo8kE9elypsmd58mf6emf29maTPCeSUCguxif8vmTrBBtCMqcR+mcU3Cd/89poAVKoUcA oNY5nU0goX7ZoAmKl1aQn6holP8kfFaAodKJogPKmlBgoUbgme3pnizanVm5B81JhIFwn7sTgN6p BDH6n54ZoT96oS76ov35mT0qDys5BiB6kTPXoVmQnFKqnEQQpGhQpHsAmid3laCgooCgpUcwpVNa DTp6BmVKBjNqCGBKEGfqBCYaCkP6B2sKc+OgoAgZmID5lYLJOG06C3Oac+PJp/hHl4RaqIO3nFCZ loa6qIwqd4gaCCK6dX+ZqJnHpXqap1pJojlUo8DHqQzIgZIWkXrJjZPaFZEqqqUKoZn6pIPpqQOE k3npqjs5cYEKn0wDq/opq0FJq/+aKqgJyaseqopFqY2SCY+X8Kd6QIkkaakraAQLcAJT0IhMiAQF YI6iyJBNMK1JOgR9mh6b8azROlraagTV+obYygTjeqcAWKy/SgTXGK1LIAET4I1RcK6YSqnCagTv KgX2KgTySq9Q0K+qCqkPSqotaQQcQAGZaAJ0WIh2aJFmx4fPmAEQS4gMu4jyiIhdZ7F8OLCLCYRH kLAL27AX+7DKGLEY23UUe7Icm7Ibq7FweIiF6LF/AJFG0K3wkhnqaAIMIIrFuImtGACwOIyjOFqm eIkbELTi2AGxSIpHO7TAyLQ+K7Aft62AoKxCsLM9S4xFG7RQK4tOa4rVqrRdJ7X/XGuLJ/C1S9u0 VCsEODs3vnqwRQCtDTCtsliPnyiMxwiK1yivv1i21XiN0ki0JmC2s2iw6tqsRkC3dru3fji4e2uN cOi30Fi4gRuzehuNhhuKiHuXuXmr5aOOFJCMfReOC3CP6Ch37LgAGWAA9JixZre6p4uPeOh1clh2 NNu5zXhroku6eGe6qFt3q9u6r2u7eGsCsou6MCuzuJuTUtmSMtkFCxkF0+sFyHpd0Su9BukE1Qul nmurGWkwHOkE40ueVusHJ8kF5csE63uFvQq+qoirPXe+fZC9KFmrrYqvzTisiQuywHqXNzq/k7gJ 14sH8ivArPq5eKq/G/i8qaq7/50qCm+7CgV8dN0AvxHcvxm8rhj8BgkoBB8cek43wnlQwfYwwZ6m wFrQf4r3fvL3dnSXfjAcdug3f/QneI+auyKICGFJv1xQe9tXfc53fQmofd8XrJ/Kw3HrBsSne0YM wkDcfd5nBE0cByZcDyi8qWjJBjQcxbIHel/cfNjnxFHspBqcxMSqwmrwdWUcAFUMxU/sxm38xmZ8 rxtMsD2sq2GQd20sx1NMxX8MyGMsyIP8fHpcmEaZx0hMBjB8w+rnyI0sw+rXxWRnwx8gpsmZwxCM xvvWpJLKxdv7BSEMwiTchD5Mpw7sBm2rCxXcqK78yprcB0t6yId5yoCayPl7xv8NvMg7HHC5bMec DMy7HApZjApXTA8oPMu8HHG2/A/JzG/LjBHHHA9ZPGDuu8Afe8eeUMynMM3wUM2ejKrYrMPMjMtL vKq63Mtmec6VSstv4AGsnIvsOs6bXAceAM9+yqztHM1xcM+58Mzj18w/J9BhcM/4TAa+uaMMmqvr GsCffKkM/M4GPdET7QTFSQUJ7QrhHMvySaP07Lx2QNEifdBM0JtWYNKnYsokOs8Qnc1zMNIkTQQo fQQzbQQ17aYbPQbPrMjpXM787AYijQQXLQU33QsqrY2/HNE+3dNwENRFUNQWDYkLfbP6rM3kLNFO LQRDTdT3m8otfdVA7c8BYNBRscDN5vvAIH0HMT3WZV3VwazU0uzWwyzM6kzMo5rW6AwK77bXfN3X fv3X2UZ+gD3YhF3Yhn1urJbYir3YjN3Yjv3YkB3Zkj3ZlF3Zln3Z6xAEADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0036_image039.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh9gAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABADq ABEAgwAAAHh3eGdmZpOSkp6dnYaGhrGwsKenp7m5ucHBwdfW19DQ0MnIyd3d3ePj4+rq6gT/EMhJ q7UhucuV4mAojiToldeJrmzrUk/8OMvwOjGqvnyffq8dTyaj2SbE5OOidDACFYFmlggsmzXYEhBQ PAivHEew9Zl95HFZfcaIu1+kQSI2rCnWbcF7BBAeC1ACBzgSeYZeYHRlZA1hdxR/bZMukheWIJiU AGIAjUhIZZChEwUPjp6nFVakEp+LWo9jhZu1IgK0UbkXuKNnnZyjwCDAYghxFaJrnZ2mC5E4ga4M MQp9wxNeMlwa3BsJMzbgDQUTBNFQF3sPCOeLW9ue1A/W3gDgDuUA2jkZG/xLBMyrB9CenRjPACyI Ue4coHQWmgFi4isZI1X7HggIITFhMAkD/xwAAfkggBVUDUqa2gCLQyccS2AeADDg1ExTqGqaxLjq AQMAB5gpk5AywEoAMgHgbIVUTKeiRz9KgIkIlQQFG3WedLkl5EiLJGKAEcCnJbGuIrXEaNDnapKr qISejSW1rhiAo6ilk9tSQVx4Q81K7eS3ZbEyNRNIKDAzIxGua9uCHZFkgb66kE9JzvFHEdMKBLS1 eizYaeDBFc3yxRzaNNPDgv2IxgwspYSfsSn6Qabb4uoKtjku6TwZHMRgx7k0UGAqMGnagF8DTj7Z buAAy5urPo16Anbmrj/bGXDAcwzqxUtyjRgPMwVwktMfJ8zS7U9cRKPn/hzeun1P9Wmx1+xpYgTX 32/9GaifYGQsgNt/+PW2T4B0iYCNK/RUMIBH9AnoigMOKPKHDIohl8eAIOAQQEIHBjZiDCVWAM5P I06Qkg1BqXLeif5NVRKLW6j4zI7nNbVibNRcthuJF/AIYIg9qRcCGVJa8Ic0fnjkJC5QBiNGTQwB YAAOD/7RQBfkeOnLHwpAoUQOj5E2pk8gUNMAlaXgoJgDVex2pl/NwRkPm25uQ6ifaDZET6HAbGjB nA+qFQ+YD+ijxFnPVVAAAzg0EOOlNMmgpC2klnBhqaimquqqrFKWWquwxirrrCycSuutuOYaa6a6 9tprBAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0045.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> <= /td> = <= /td> <= /td> <= /td>
• At a certain point the increasing
fluorescence will pass a fixed
threshold value (=3D Cycle threshold or
Ct).
• There is a inverse relation between the
Ct and the gene expression level (high
Ct value =3D low expression).
Control
Experiment
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0045_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBgQAAAAAAAICAgAECAwL/jI+py+0Po5y02ouz3rz7D4biSJbmiabqyrbuC8fyTNf2jef6zvf+ DwwKh8Si8YhMKpfMpvMJjUqn1Kr1is1qt9yu9wsOi8fksvmMTqvX7LbbJFDE3/S6nSTI6/P3vv9f MTcXMAhoeIhIeFCY2OjoNsj4OElZpleJmeklKanp+em0twdKWrokaJqqqtS56vpaIwo7SzvTWoub Cyen2+vrIXr5O0xMwVmMnMxwrNyszOwcTVx4K21NO3qtvc3d7f0NHi4+jiRMfk4Zib7uqM7+bihY DU9fb3+PYo6/n+bO/29pEcCBY/wRPNhFH8KFXOYxfNgkG8SJURxSvHjEIMaN/xklcvxIxCLIkTo0 kjxZ0iPKlTdQsXy5QyTMmSVc0rzpIo5OnDxZ8JHZM6jQoT0UEj26YSdQpEwXKG0K9YLOpVGrWr2K NavWrScYUeWKExrYqnwQfB0Lc6pAtGTlnWX7MhhcsrzmNhVrlyjevEPl8mXq96/euoKF7i188zBi moEXO36MUCVkxgbUTk4s8O1lhu40b45cVtHn0aRLm8Zk8rTq1axbu37dQjFs0I1nP6QW2jbF3Loh Gu098Tfwg7g9DxcXjPdxgLKX4xPu/F/q6PymU3++9jpB6Nq7e//S/Pu55MbFRytvnhv39Oiesrfn /j09y/Lr22ey/v436/r3Z//vT448AMKD3oC+SGagNwUmeOB/DCqI4IPaLCghLuFViAx5GG5D4Yaz dOjhKvGFaM2IJJ6X34nD8KfiihG2OE1lMHI4Y4023hgOiDg2ktyOv7DoIyxABvlKikQeKd+QSJoi i2hLfvikizpG6YeSVHpi5ZWZZKklasp1CSZ1F4Y5iYZklvLimZpwqSYibLYZj4NwYvnlnHaedsmU d5LxlJ57htHnn6iJVqegbpplaDoJ+JloQ2Yx2ugWaUYaz06UpgPppY5qymmnnkqQ6adVmCiqHaSW SsekqK7x5qqAqurqGTbF2keotLIC660F6Vprobya0eqvWXQmbD89Fisrssr/LstsszTk6Syfilga LRiBVmstobZimwM13Iph5LdTnCouFuSWawV96K5bT7DsGtHku+bKKG9C9Zqb671J5KtvOdP2O+q/ AI9LyLYDv2DwwfnUpjArcjYM8YoPR9yRMAlT/MHFGNfk68ZCuOvxsxOHHISAJJ9cJL0oF3HuykVR 63LMMs+8Wrg0ywDyzfmMrHMsPPf8bMdADw3Jz0QjzPDRMRCrdExNp/R01P0QJnVOx1adk8pYi7w1 DPF2HRvYQWvcdcti4yHw2Suoq7YKZINtdtsgxC03MDbXrcHbcBuNNwdf9z3CrIB3NTjHfxfeQc6I L85446Y6LgLdkEcg+eQP1vBreQN6l8135g4c7rkxWoeeN+kYgG46BJuXLXTqTnXuenaCx77M1bRr fnvuugOxutK2747o6MAvCvvtdw+PPNXCD29m8pUd77rik0sPOfXVt+589otin7v1jXvPOPjhc6+9 9tCnLj7iTGffvPO9lw//9/EvQn7s9esOc/z3Gz9/we83XTnaBdB+BeufAdfyP9+lDX4/OaABZ8e+ BZavgQ7s3/lMN8DoSTCC+6sg+pSHvPYlL4FPg6D7Nsi+/HlwhSxsoQtfCMMYynCGNKyhDW+Iwxwu qQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0045_image040.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh9gAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABADq ABEAgwAAAHh3eGdmZpOSkp6dnYaGhrGwsKenp7m5ucHBwdfW19DQ0MnIyd3d3ePj4+rq6gT/EMhJ q7UhucuV4mAojiToldeJrmzrUk/8OMvwOjGqvnyffq8dTyaj2SbE5OOidDACFYFmlggsmzXYEhBQ PAivHEew9Zl95HFZfcaIu1+kQSI2rCnWbcF7BBAeC1ACBzgSeYZeYHRlZA1hdxR/bZMukheWIJiU AGIAjUhIZZChEwUPjp6nFVakEp+LWo9jhZu1IgK0UbkXuKNnnZyjwCDAYghxFaJrnZ2mC5E4ga4M MQp9wxNeMlwa3BsJMzbgDQUTBNFQF3sPCOeLW9ue1A/W3gDgDuUA2jkZG/xLBMyrB9CenRjPACyI Ue4coHQWmgFi4isZI1X7HggIITFhMAkD/xwAAfkggBVUDUqa2gCLQyccS2AeADDg1ExTqGqaxLjq AQMAB5gpk5AywEoAMgHgbIVUTKeiRz9KgIkIlQQFG3WedLkl5EiLJGKAEcCnJbGuIrXEaNDnapKr qISejSW1rhiAo6ilk9tSQVx4Q81K7eS3ZbEyNRNIKDAzIxGua9uCHZFkgb66kE9JzvFHEdMKBLS1 eizYaeDBFc3yxRzaNNPDgv2IxgwspYSfsSn6Qabb4uoKtjku6TwZHMRgx7k0UGAqMGnagF8DTj7Z buAAy5urPo16Anbmrj/bGXDAcwzqxUtyjRgPMwVwktMfJ8zS7U9cRKPn/hzeun1P9Wmx1+xpYgTX 32/9GaifYGQsgNt/+PW2T4B0iYCNK/RUMIBH9AnoigMOKPKHDIohl8eAIOAQQEIHBjZiDCVWAM5P I06Qkg1BqXLeif5NVRKLW6j4zI7nNbVibNRcthuJF/AIYIg9qRcCGVJa8Ic0fnjkJC5QBiNGTQwB YAAOD/7RQBfkeOnLHwpAoUQOj5E2pk8gUNMAlaXgoJgDVex2pl/NwRkPm25uQ6ifaDZET6HAbGjB nA+qFQ+YD+ijxFnPVVAAAzg0EOOlNMmgpC2klnBhqaimquqqrFKWWquwxirrrCycSuutuOYaa6a6 9tprBAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0051.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
Hybridize with labeled anti-sense RNA
<= /td>
gel analysis
       C      E
Degradation of ssRNA
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0051_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBggAAAAAAADPM/4CAgMDAwP///wECAwECAwP/GLrc/jDKSau9OOvNu/9gKI5kaZ5oqq5s675w LM90bd94ru987//AoHBILBqPyKRyyWw6n9CodEqtWq/YrHbL7Xq/4LB4TC6bz+i0es1uu9/wuFw1 cNTn+LweNej7+3uBgoMZd3cBh4SKi4yIC4mNkZJyh5CTl5hpfpmcnWKWlp6io1J/f6SoqU+Gqq2u TqGvsrM5prS3uDexuby9dHa+wcIippvDx8gYoMnMzRDLztHO0NLVyIm71tq4p9ve3+Dh4uPk5ebn TMbo65iV7O+S7vDziobZ9Pj5+vss6vz/beQBHKjpEcGDZwQiXBjGH8OHYO5BnBilG8WLVSRi3LhE /yHHjx0tghyJRCPJkz48olyZUiTLlztYwZz5wyTNmylk4twpo45PnkBhALIZtKjRo0EcIl364SdR plAfOI1KdYPPp1Wzat3KtavXrysgYQXLkxrZrIAYjD1L86pBtmjtrYU7sxhdtMDuRjWrFynfvkft AoYqeLDfvIaN/k28czFjnIUfS57M0CVlyArcXm5scO5miPI8f66c1tHo06hTq+akcrXr17Bjy54d wzFt0pFvT8RWWjfG3r4pKg1+cTjxhbxFHzdXDPhygraf8zMufWDr6gCvY5/+djtC6t7Dix8Tffy6 5srNV0uvHhz49uymwtcnfz4+zfbz64fyfv847f/+/dddgOjYQyA97B0ojGUKipNggwsOCKGDDE7o zYMW8lJehsygx+E3GH54S4givlJfidqciOJ6/a14DIAuvlhhjNdkRiOIN+ao447lkMhjJM39OAyM QtJCZJGztIjkkvYdyaQqtpj25IhTyuhjlYI4iaUoWm7ZSZdesuZcmGRit2GZl3iIZiozrukJmG4y Amec9UhIJ5dj3qnnaptcuScaU/n5ZxmBDsqaaXkaKqdairbTgKCNRqQWpJF+0Wal9fyEaTuUbiqp p6CGKqoFnY6ahYqm6oFqqnhcmkMBsMYq66y01mrrrbjmquusQOzq66/ABktrZXb2UEAWx/qQrBX/ yx4UZRDNVhGtDtNKUe0/OkGL7A/XQtGtdUN8+4S4NJDLhLkIunoDukuwC4O7SMAb3xHy0sstFvWe l+gO+RbRrwr/ChEwOXO+K4EACCes8MIMN+zwwxAzLMHAJ3wb8cUYZxxxBBQ7+FapGXR8r7L44vas sduSfIXI7tlbssrMfsYyyjBLK3PKNMe82cw88ByCz68iNxQRFmts9NEbTzxyBEg37XTCHH/niKba vpyzzc5OLWWvOPdsNbgGCtw1v1+DW6zXZVObtnX7qr02DkDDTSzVXL+9rt37rIr2yktjLXXbQTP9 9OBHK12zA4QnnnHUO4/tts5S2yg23jXEfbfQ/0pW7njgkGe9dd18Hz6F5duAvAHpNqB+OuX5nNy3 33vDDl3mmrM+g+q3z/256KO/3jtyWk8e+tVU4N4hIqavbrsMxsfQ/DThbi738M7mFrvsj2Of3dlk L++893Q9bzDvUYg/JPfZaz9957Mbk7wF5rsQ/wTzr1B/L+9zcD/Avlv7EO3lkt7l2Ge23RGveP0r H7EkVzXqdY+A20Ne9MD3gv2hwIIrwWDFErgAxXnwYYyLXP4woEETlHABJxxBCk0UvAZCcH3qwxbd OOgtGjphhR/B4c9s2AQdbsSHH1ghEDswRIoUUX88PNf/AAfDGKaOgvNiIOheOEAn0gd9nKPiE/8d WMARwk+AW9Ri3phYRSsGkIuPOaLyDtiAD7pxYSH0nAGvh0Dy1RBz6jojGpv4u8ghaopmzN0egZdE JdpxXL/xouEGGcZA6kWNGhAiFM+DGDb675A3NFkemQfG2omxdVIEZB0teUfQTJCRnnTkPFyHyUxi 8o2wFEAcoeMyVOpRlaskYyNxWcFJFqiFouwjHS/5tzk+8AGxfCP9CqkESH5Jgqf8pCCleR8jOJOE zEzCNTOhN34J65vgDKesuCXOcppTV7pTJCk9sU33AJAL7ewhV9QZzFHE80JYDMM92zXPTXZhn82c ZyjRAFBtgoWex0xFQVnESjIstJZ4GagZHmr/zThR1F8WfcVFU7PRaEa0DR0VHl5meIaQurAq3RyD SevJFH/+U6MCfcNKs1mWfIJhpq1UjEu3gNN1HsaYDoXpVxCaPlT01BcNLcNRE0oVovqUE0vFny69 ENUsotSm/xynUbV6Vck59XaxagVXmxqkksJKrGfVylepVVW4tXUwb92lfc5J17rySll2zes5e6XX vXJhrQowqSR9aT8CGPawiE2sYhfL2MY6lgAbLWtODflUeSrrsZjNrGYVe9GC9ZKw/ANtCgqw2dKa lrGdxWoqeSm/TpbrtLCFbWSnekvWtkCw3IqtbjcbV6uOcpjE7Nluh/vY1D6KpaUErgIvS9zm/3I2 I5KtLGWVm1x+Ofe6h+3sOzkp2gu69nbYxa5xgepbYTLVvNQK73XHC1gG4Hay/GSueonLXo/+9rzB te586WuF7X6vuxsEsAn3y19avFe68TUWgYc72/a697vctSV4F6zb+orUtiw4sHApHFsLn/S+RUXv qzjc4YyotrYgLm9+00vi0zaYaBD+r4SZ12IX30LD+F2ugmtc2hfbV8R8BPK6eNxjE0uUuoiEb0Dl S2TMWri9OA7xikfc5Mxq13pS1jGSXbnjKhfXyBgVcAmiTGUvO7a3QZ5ymrWsXzM3Fs1lTPGaq8ti N6P2VD9Wc5yF7LxlkdbOd+4vbSNMTRkX2v9+Yf1zYi+wYO1WVMwkEGxfz7lYRjsWA4vtKEkRvGRO G/RVia60BRqNhUEbGsP2izGiUSjqCpC61Hlmc5bpXGZAJ9bHF5bznvWcOltn2sSb3rJlPR2v3Pr6 1sAm75yTTGyItvnYhk2tqU+ta7nyuXLQRiycrc3r1VYb29mOtpEdjEJVf3bGzgu3uKmQUhXL2t20 hpu6IStoZe/63cvm8obDvW1vXxvF/54wv8cNY0ir0Nzym7e07c1tfN873kNW92wZ7u9uA9ziAs92 vy/u8IZDvNcSFzS5A4vw25Y8wwpP9sjJnO9h71vjKo/1xyvecXAPnN3QzHXACY1qgKXcwCdzT7XB RaBomOP8uDQttpI/3WWjT0FNS1d6sx/ddGiPt+DoHl/WK/jzpx9Z2NPN8cxfG3KcB1vszAZ7gl9u 9XqvPOih3XrCyz6LSdtdWHy9u95zlfe9B4tVgA+84AdP+MIb/vCIT7ziF8/4xjv+8ZCPPGASAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0051_image041.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhvAAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABACy ABEAgwAAAHh3eGdmZpOSkoaGhp6dnbGwsKenp7m5udfW19DQ0MHBwcnIyd3d3erq6uPj4wT/EMhJ q7046827r0nyjWTpnM6jDBJxPgGFntbgsGWu6+Hu/zRAIOEoTBYpASXgiFkYjsVvShE4claqdhcE WBsyh0h2ETQej+20cC2x1fBPFzB3QBVki+GgcBDiWA9tIwKCgIcZXS54E1dEUhKDFGAGDggVAQ0O lRMFggpOSwgwmpdCo2ZjAANEDThEKBKeDqATA5oJMbA0AQtptq04vWlID38WAlBiwpqcEpmbbckn CQOVJ4x9fkEDD6qRXoJGdDVSLmCTMUwSNgFM6GE3AFAGAIJFBeguBwB9OF3t3rVoEAMBui73Juzr J+8ev3MXNAVw8YugkEEW1wGQSBHAATGT/5TMcGWhDRN5kiQscHIiFB0GIGakjGTSwUEHSiZAYQeS zqBdQRL8MvCrzqCdq3p2mTMhwc1IMCvYAfGU4bgC9XwCYDMuz9YUGuOhMCUBF1mmJQcFYbq0Tdu0 cBupnUtTboYCsMqKIbtxbwW8QSACUBUEiUtyEyoZonCAn4SP8CQoM9VSw1utYexi9nm4cmbNm9fS tZApgYujJ/hOftbAdJc+BghA2ixUagUkKRvk9OJZIRIJRGAWuhDESlSmRP4d/zl1+ODmRSV1SQ61 rvUKmq4DkJ2Su97rFBns9lzowTgmh4lQWPDteVQ6MczImjE7DD8ox5i6EPHxmKAAeLCBAo8kAqYA yX+MKAJSf9ehxdlJMTThBTwSyvdgb0QwQhM3MsVCwS8MteVhEQ1YJAFRUxEXTQPjoBDZKrjg8FUu JwryHgAozsYGjTPYEuOGV/T4l01DNPAHiSbSkY+JbBDk1DFfQbnDbohsVuWVWE6QSZZaOMjll1sY 0BWYOnhJ5pklCAIimiQIyeabJAglpQQRAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0051_image042.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh4ABlAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADc AGIAhAAAAAAAADPM/3Bub4KBgpKRkp+fn6urq7a2ttLS0sDAwMrJydva2sz/M/8zAPj4+OLi4vHx 8erq6v///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/4CSOZGmeaKqubAm8 cCzPdG3fba7vPHr/wKBwOJsQj8iYMclsJkXOqBS4nFpf1avWCd16m9kvMywu48jmdFH9ZLtl3bcc La/R6984Xn3fw/p+VnqBYoB+hoRcfQKMjY6PkJGSk5SPP4h4mIlPmptInW+gnkKDo4KmSqhapapc rQCirzSsMJW2t7iUl6+xsnC9vqmtwMGwxMXHhcVgyb7NXs/Di7nU1ZW7w8tj0dnd2ke039iq3Kjh AA4O6C/q6evr7urw7Ojt9PH39vD27ji84uDKmfsHcMi5gmu8Ibwk0FRDKQ83ncO37107fRjrWdQo 7+JGfP1sRHS1kMrIRCfH/5Q0uZIKwZZ2UgaS+QmmyD4UPXbkeC/fx4w5eYa089LmL6NEFc7YGbSp T6ZAo1aUdxPprKJWjWWFg9XqOWtgwzYaN3ArFpp70BJRO4ftnK5IDyqacABWAmNGDjww4zZIxBF1 J9wdAUBvGblcEuyFFfiFArqH4V4RvBjyksd18zwTsQDCgswiCGCJQGAJAwURIitl1vnzWdGwSJtG XSjahAETECyZcBpLgQdLIIgwoGw1GNy6sfSG9Tv4cM2TYUmXTlr64xd7C0AoTi66kd0Aqhu5DiD7 dmibUxggAUACAOEKHjfs63KKivWE3cOXj97sH8k2IVYSfWRlJeBCBPrj3/+BCCUokn9aQehgUmb1 YOGFGJrAS4YcdshCHRNC+KCEIsoSIncllrVgisbFxWJ3JL7okIgnyjjdijZ6UiM0OepIY48S/Qgk ITtuUSSLR0Y3ZAwBBACAk03yJeSSTQbQgJVYShkjlQ106WWVquE4ZJNelulkmBUu+WSZZkaZx5Rm NABAl3O+ICedctaZ55132jlnn3oCKuifT1rJ5pVpJHkKl22eieZWirJR5aRuPmqgmlDK4CiKkKp5 CJyehgLqmJlW+uaWpGJ5JaKWeuWpoY226iKVsJqppZhA1sqmqauMKiOZh4LJ6aVp4OnnnoQia2yg dep57KDLrrrrpqfi2iNkpcLemiaVTxb6ArU8ohqqG5FCNC6Ivp4rq1HlWkusumy0SxK8fKRLr5H2 3quku/riK26//vIL8KICD2zuvwbbl2/CKlXIHsMQPeyVxBDPJe8nDFZ808UYcxyXx+BoTPC2IgMU AgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0052.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
tissue cryosection on microscope slide
Hybridize with labeled anti-sense RNA
autoradiography
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0052_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBgQAAAAAAAICAgP///wL/jI+py+0Po5y02ouz3rz7D4biSJbmiabqyrbuC8fyTNf2jef6zvf+ DwwKh8Si8YhMKpfMpvMJjUqn1Kr1is1qt9yu9wsOi8fksvmMTqvX7LbbJFDE3/S6nSTI6/P3vv9f MTcXMAhoeIhIeFCY2OjoNsj4OElZpleJmeklKanp+em0twdKWrokaJqqqtS56vpaIwo7SzvTWoub Cyen2+vrIXr5O0xMwVmMnMxwrNyszOwcTVx4K21NO3qtvc3d7f0NHi4+jiRMfk4Zib7uqM7+bihY DU9fb3+PYo6/n+bO/29pEcCBY/wRPNhFH8KFXOYxfNgkG8SJURxSvHjEIMaN/xklcvxIxCLIkTo0 kjxZ0iPKlTdQsXy5QyTMmSVc0rzpIo5OnDxZ8JHZM6jQoT0UEj26YSdQpEwXKG0K9YLOpVGrWr2K NavWrScYUeWKExrYqnwQfB0Lc6pAtGTlnWX7MhhcsrzmNhVrlyjevEPl8mXq96/euoKF7i188zBi moEXO36MUCVkxgbUTk4s8O1lhu40b45cVtHn0aRLm8Zk8rTq1axbu37dQjFs0I1nP6QW2jbF3Loh Gu098Tfwg7g9DxcXjPdxgLKX4xPu/F/q6PymU3++9jpB6Nq7e//S/Pu55MbFRytvnhv39Oiesrfn /j09y/Lr22ey/v436/r3Z//vT448uQwAIB4DFgiCZK8QiGAH6E3CYINJ/TdLhBJmIAsuFl4o1S8b chgIYQuCiGFytHxIogQPPoJiihGs2EiLLjoQ34kzhriThjcak58pMu5IGIyG/AikWQq6QmSRlS15 oJITCAlIkk56OKU1UlZJ4SpXFmlihVguk+WIXwbZ5JgJ9FjKlmbCouaN/KXS5owZQnlHnC7SeYid dyrH5ppmhYmkn9nh2YeeJL7po6CL8CmmolQqGh4ohoJIno2QoknKpBwimqajnGbqKaBaOkoIo4GS 6oumEl5CaKGCPtWqHaoiCGuZZuZoqiqz0vrniQP8uqt+XtUCbLDCGqnhr6//YiqpsQX+ZOulqPoS 67TWXovtKdlio0i12/bT7berHCmuJ5+W2w656FZi07qpeOuuGBnGay697+ZqbyPn5utHZ/ym0+W/ 7QhMcMEGH7wNqwgnUuvC8YQLr8MViYavxG1QY/EhzGaMRo0c2+Hxx3TQJ3LJUO1rsrzqpnxGuyzX EfHLHW0sc0I019xQuDivEfLOAVXsc0FB91Pb0GGgbHTS1YmqdEPZxNw0DlBH7cPNVEOB9NVPZK11 RJVN3XXYtbgsdhY9l20FtGivzXbbH1vtdkhMx43f3HSzYvfdScCt90nO8spkn6gGPOrga4E9xt+H Noot0Jgo3vcNkPcXaSaT/wtLOJzTkn2qtYibcbl98woeqi6hi+74J6fLd7aupLaueaipq254L6un B3vsr+pMrKd8U6Ls7o/6yfXjl/4OvPCfq3E7e8un0Xx3mXu5ZvGaRB95C9hnz8L23KfgvXPPuxH+ cLmzWX5v55M+5cq9Vzl+HenPZj2o7bv/vpKcDzO/bfG/0T/WjC4ZAVTN/+pUwNMgT1cJJE39tNTA z/jLGcFLUaWiUUFKfSOD33MCBzvYhAiO5oCFEiFipieNDx4rcBts0ANtZ8K8LNBDMQQhBlSIOxGF A4ffueA5eKgd/G0QiNF5YTOAZR4jUpCIwFFiCpnov9n9EIo2HEKxljNDccMgUTdO1OIVHUBFuHQR HcXi4BYfM0Z6lLGMkMniQL5YxSSEUYxSjGMO0mjHFEwwj0LwIR+BQMI/CnKQzggkIWlUx0O+IJGK lEGOGglIQ0LSKZOsGiMrqUfeYVJqmtykDUjmyVC+YX+ihMH6SqkCtaFylR1jIStN2clX5uSRsqwl GNxoS2PEMpf52CUvd3HJXwpTbmcaZldQaMwPSDKUpExmgnzpzATRMprUrKY1r4nNbGpzm9zspje/ Cc5winOc5ARCAQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0052_image043.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhygAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAC+ ABEAgwAAAHh3eGdmZpOSkoaGhp6dnbGwsKenp7m5ucHBwdfW19DQ0MnIyd3d3ePj4+rq6gT/EMhJ q7046827DInjAaDYKcpVTuiIte70zI+zDBIxOwFFz5bBAxcrGi0OoCf58MAqTFbqKHnGlAHFozBJ 1ASUwKNnYTwSnSbVR1V63G31USBfA9z0hu8xlV0EDQ4mdkZ1aYYbcEeKMQWIi3VwD2YLbBYGBwsP BISFV48ZjEWiHgJJnXdyOpV+WmgSj3oGDwihbko0GD+wuLuSF0BeDQGzM6yamzMBDbQfCA6AKSsT BMyOatPLD7MSAmZ8OFq50xIDWg1EK14OnEoDDn2wAKZb8hUDaDp6GpF39hVy+vnxJxDggwMAhOg5 wIdCAzAzcJgxAIBJgQJ6onxwwAAAQzUa/4eRUMMsgA4TbjQC0IFQEw4mCPWlmoHOAraI/iokIKMs FBs1oAIO/JeT6B4ZajRxAVCAYqoPD/bNAIO0yxheVe90pKBAKtBIcsyUa/gUKxBHSy0xrSHG5o9m uo4aVTu0qN2gAdXIBNDn1leDE05hHcxMQS0KBcQNXuyXcRMlXsjUnSV4wgGEEhjuA0YXlNGCRUk1 lqDJAIFXZe+gNDQ6tZtvh5cp0PE3q+vaT90oGGTUi6GHE+hc5Vz3kdDilh7rykutBgOqZcW8UuSG GScxzKl5kcBssCQ5WohMcpyKjCkHS8VIrlInQTy+423m7IfoOEF7yu/bJc/iASsJSXDBAIU0iy3G UApCROVdD4DwEgB2PSQRACtu6JACQ5zcNpMaCb5lyCDI5JULYESJsottI6bIiC8jrpVhN74lQBUN m50IgAGCBODAThvegZFIaw3TFSeOKNCDjQMUJl4uNsYAHSpQRrlMlFRWaeWVWFZgQFpZdunll2AG VkOYZJZpZie7vXgmmBEAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0052_image044.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhIgEtAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAAAc ASoAggAAAADM/4CAgP8zAP///wECAwECAwECAwP/CLrc/jDKuYi9OOvNu/9gKI5kaZ5oRq1syxJu LM90bdOWoO987//AoFBnuRlxsKNyyWy6csOodOorOq8N66xz5GC/Rih1TP5pwc7zShNhU9yODXou IZTveEGS3tyv1U+AABcOARADWYJ8V2J5jkOKi0gtkTJaioYAA5uIEYZ+kn12j6RBlaGBT4yDCwGu r5ktmaCoN42luES0tZa7dV++Cp0OiLCvDMG8lLnMO6fKE8ltwAubDJ2c1QCxENLQbaPNuM/f3d7T q5rCDcPc7HHlgeK55PGJtuep+e7XWfZ/88bl+1dhSb1ofgi1MhYr27oKA8uFC/joIEGLLzAqUPiA /yMljwQ7TqSIR2M8kxtPpVBZYlBEaLdIlkH5rR6XlHAQ5vTyx83NkyNlkqFZsycSXx75aUOmCGTG l2mEOiKq7BlViEwLZXKozhxEqCJrxZQ65arYc2CjbVTAMMYwVml/LRpLNopZVDaxRFKKzVpXZHrp 0K0rxGnIbqrSbWPgStgwa28RBwYTlLDduDBfYq4DwxCspQOUyk1DubJlU5t5oUwN75nov4BZ32Nk +rQZ2XjxKTmjhWFDrnH23L0b3HZZ4pKGr266fBeGLcizGD9++A/tnXJ6Pmeak7OKr9/11p7uDHdy 89rNjeDOfD348+QhoZ97EeN2kfYNoxkcX090Pqj/HRUGKGYF2E1/hc0nmII4iLIWEwwyhSAQBu4X 4RarKBghf/HpVx1gF1EzGYTjdVhhadUxuGEfE1J4ITAv2qAhK6QZVCJ5J1L2YVYGYbUbbhzimKN4 OwaHmVNUERfkdB4WOaR4TeLE0n3gRBnDjUIWCY6WgXgg4we0tVhFjCNyaSaJYvZgZYpknllfmjys ediTbv6zJJNtXrfSnnz26eefgAbaXZ0KJAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0052_image045.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhTAAgAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQBI ABsAgQAAAJnMAMz/MwECAwJ7hI+py30CBHS02sukxLz7qE2fB23jE55kKI6aur5qALvpGdBf+wVm 3bmNaLzcIWc0IHU7oYeJgAIzLOVROs2CTNgkYKnFGSbYcLhsTquBSfAVvY5Y4HGLU0GvU1g8/bTq F3YXyDbRRzhziDizONeoxeT2hfToWMl2yVEAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0052_image046.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhIgEtAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAAAd ASoAggAAAADM/4CAgMz/M////wECAwECAwECAwP/CLrc/jDKyYi9OOvNu+fDJ45kaZ5oalJs67bW K8/RoNh0ru8OIfzAoHBILBqPQAJvuVMyn5OBFAetWi3IrHZLjFm/EK8OcyUDplKweuzjut9F8Vot Z3EqG5imYZle5oAUWHCEhHWBUIcSfzSMPXUBDTZTeE6Il22FmlyWl1WdgqBseA9oC2hUZwqKnomZ m7BGrK05sw+iS0piAby9qKeUN2mrtGWxx3G4xY3KEc08tqbAUgG/C8/LesjbQbbZeo10OpFn0sTf tdzqAtjogmN07QvkDuYA8u5469ve+c74zr7okkfPjj9t+471O/joyUI7TmxA6sXLxcN8gxLCusjw /5pAjpWEDbtQ0CJIdK80ajp5kKXJPdfyTBum6k5MmB0XpVQJxyVGfCkmmECFgyYfFQARZeRpKGnH hY5eRD3aqeQpkcEenTSTbSnTNz4xQmRS55BVCGjkhCVVbOdXTk4ZQi1zTwnFXqWMqqpw7lMrr2+3 rP32MC6MCFat/RMXCHDgLIO7AjR8WEHBipKyCuvBeI3jx0cid5VB2SCASBQdnMVKRTRnz6DBlnbH cnZAgjXM6RLo2W3sZDlNNrHNti6Du5hz4xAj2vXR34KJE3boc+oqlhIVhXWuDzpk7p7A49Rq/VaG MOPRcyVf3qF3LeAxSYcok6qIm+fZf7heH9Dn97dJzNeWgLmsJ1R7/CVloHwAhkYgLQ8OVyAog8WH XoOyWNhYhPD4ZZwrjWGYYXDgyAWGhRxeJ2IXKW6YU4oonugbhho2RiKMMBqzohAIklhccBzGmMiO Q9Q4h5FkfeKEc6791yCSnvlonm2HVCigkwD2KOWHW045WY/50XfljE9CKWOXeozAjJpXEMlji/7B ieaWWL5n5olz5qmjm+zcSZeegEJDZpZ+loHUoYgmquiijDa6QqANJAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0017.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
RNA isolation
RNA isolation
reverse transcription
reverse transcription
hybridization
transformation
labeling
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0017_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAAHBub4CAgIKBgpKRkp+fn5n/mZn//6urq7a2ttLS0sDAwMrJydva2vj4+OLi4vHx 8erq6v///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIDJo2LkJGSQAOVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbFqZQLSo0aNIkypdyrSp 06NAXznTMiFO1agJRfh0cvVNV6yrtjL52oYsWE7EtpiVcaCtW7cv3srFsfZspkZ41dqQy7ftir58 bdS1OwmT2CWDWQDum2JxYBqJCf+LrMLx3BOWL8+gLDmRySucUWSGi3m0X8id0Zo6rCR06dGNTddw nXpQXtZJaL8GrNjyDd21AeHFnTsHYxjHfwdHSHlxXN4tHLMAvlxdYtiVM//VnoJ6dXODTR+IjX23 bxTev5csQbxIePGiZZs/fyK9+jvsqrwvbwL+fOno3bcIJiS0N8R+3P0Hnf+CjwUooGegCCVFZAmS t6CFF9b3oGcOGXigCw2CGGJ0mq1g34ZzdJPFifRll+FmKB6SHxX28cfgW4LFaMiMU6Tn340lxnCi jm7wOGEN4o3X348yDElkTFOBhqR8S1LZ5JOEqIiFj1aW0KWQWApiZBQsAridczmGKRw2UhoXJImk 0aXmmuxtadWcwkU51J14WvfUn4AGKmhSffqhEyBOZpIoUMQQiOgxi/5kkIdrRBqJpTspRGkamC7S KU4CbYrGp4mQWugYph6S6qlgrFqIqytJ2Aesg9DKql7G2HoraEYFA9Wue/wKTK9EjolHUcUg+2Q3 js5KVK66ggRPs7NCiif/tX9EC6wdn20bY7feqjetqOHCxCy55bJkbLqdgctucLK+m1q88kpGb72E uYvvvvxKtG6/PJ0LsL0Df6fvhIMmrPDCxBZcxm24euUwMqtF7Ia2E3MTIRcYu5fxsHx+LMbBZIYs 8hf3QtExESufPEPKT7QshMwui6MVuj/QDITONXdicVk9o0xyzCYHnQXOMxdt9BUwc6X00lXo2abE UB/9Bc8+YF11nVzvCYfWW5/CrJ1fh8301U+bDQXSO6etdhMQU+X220vEveLcdCdx6NQX5y1F002A vYPgYUvtNdV+S6MVx3gnrvfPbBDuON9AT35p45YjwXYOksuZORNjUx75/+d1eyR32aQ/vvjpiKd+ xNBEo+66IZ0rN7sRgI8VRFEJBDDBAr4/m8ADSd9exFSbe77zAsT73vtVDEzQe9vGV4Q25xM0AEED 0xNFgAgTREDAVQ4wEMHu1R+P7eF0CTCBAldNUD74BTxwFQREGUB9+kHkjtjgvgtgAMUXwOiJgHgF gMD++PcD/7VGB0wxQK8kEAD8MSB6nard0hxYHNkxsIHr0w/mPjgHDc6GhA1kE/sqh0IeCEx0lWph D/Y2FIbZ8IZPeRSWODjCV5jQHzz0oK/CFMTWDZGIIaxWrmTIMmstyzCvcqKOIJY8VEnxWxWrohh+ GMNimWJjiBLWLpAyxf8CCUKMukCjgFChxaspy1dv3NC0avUsYcQRRTT0Qx2TxUV+QLFWV9xhG5l4 jEES8pCITCQQFbkPuzFSHo58ZDvyKMlyFLGSyjAcJsmRlk3Ow5CeLIUmQ7kNUJIyIUk8pTUuyTIc uvKVDaMRLGeJw5FoimxGPJIQMfLCFY7Ol138iCltl8uS7fJdfQQTMNWQTJA08wWCe6YLpLkRak4H hsyU1h9Z17dljgAB4AynOMdJznKa85zoHKcKrEkKKkIumDTq4UDcyU0WxvOY/vpiKo1ZTJXJc55m rOcvRaiCdBr0oAhF5zpJAjun4TN2/ZxIJL05KmxyypYVEyg8e/RPgO7/k5/dJGgKEkrSkhp0ofxi J0pFGlKOsPJDD3VoRP1FSV3ONHAdBUgn7xZTnBbUpEANKjhXytB3ZpOiZ1BpO2sKUntytKcQYWpT B3rPm0Y1ozy16v9+KtSuJpSoG5koS51q05aGFYwaPepYqYrRtF4UqWZQ6ihCZ9GKwrUMcm2I6bJq 1rKSFZk5bSJUGfLS4mn1gYNdCPJw2deplsCrkD0oWHl5vcN20LIIaahPMXuEvJrAs5soLPp6cJQE /C54VRmeYNeKPd6dFlmqNaxL98pYAAbAtM4TYPSmN1rWck4EuJWeboXb29mitbY1MC0FBRjA8I1P BOU7n2yfmgPlgm8E/1VxLvnMN115JeoB37vudSdQv/vlr7uOpQF4scte8tpPBPibgP4WeNYvujUG xIsfcLMbAAMGAIEKLC51cZBf8Qa3v1cBsIApm5f7wgB+DUAtUUz7LApaEIMLTu8MICxh6SHLwhO4 4B5Jy9AG8/Wv/kzsrkBLgmgGFk8sZu9dyRDjg9SYuVVt7EU0qzsVI+HGN46EaHdGyyIvjFdGTnKg 2mrfQAS5rvcZsl2TpSYe0+HJM1ZlCbDs2wdJOamB/JaWdEjlHZpumMjlBZc56eQwI1GPaqwFGbU8 nVim0c5yVCEf8HznEeORrs5aM5n8jMfVnVHQCLsWIRCt4TVKlc7LQP8zpDVh5UkDQ6yWfgamMx3p 43KaGpL+NIRMLGpNY7XUzHg0qncR6lVnhLauNsYoY82LndK607eW9ZhzXWtY8/rXwA62sKPS6mGn 5NTGjsWmky3KSjNbyM9mta+j3WxnUxsRtr62VLQdFlVze0DfVvZHww2JL5P72Ksr9rkDAuh1Q9vd 8I63vAM67wHtut4ymja+d6TvfWdk3P4OOLH1LHAxtbvgeQI4wm/R74X3wdwOp5ihI25wdVP84uqw NsZj0vCNc6vjHqcDxEMuNIuT/OSgVjjKOT7xlX883S7n1sFj/gaT0/zmOC9jzuug8Z13QeU+H0aT gw4HoBO9JkcvutFwk/5zZDMdDct++sg8LfWqfyPbVg+I07Oek6Fz/eum9jrYe7L1sXthm2ZPu65t rnabkbrtXIg63KkwnLnbXRf/ursTeqn3qPX97C3/u9/fLni6E77wiE+84hfP+MY7/vGQj7zkJ0/5 ylv+8pjP/AhCAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0017_image047.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhxwAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAC8 ABEAgwAAAHh3eGdmZp6dnZOSkoaGhqenp7m5ubGwsNfW193d3cHBwdDQ0MnIyerq6uPj4wT/EMhJ Z0rUKKcG0JxXjWRpAtcJBMszsq5KwbKUVrSN1erNU45gkHEQVHwERwHQSi5bv+jIV3oER1ZHNMuj Srg6acn7CxoBg8cjYGJoJ26xVPCOXkf3cl0OyPMBdH8VfgFqZ3h7foImA3s8ihKQKpJ2jnKNixOK CA4HJX6UmRICVmKSoZ+WUqg1pKpyinQKEjQFQkG2txJpDgxsKwcPAgo7vL4rUAsOD0sJujmADUEJ BJqqV8oKAZxDEm5KfQ4BG57AwsTIMQAFG41v0LdBLgLSDtQoz1ATBM4K1elMli2BdAdMOCB1kgQI wOFLkAEDZilkOCuLgXUNDzqssyGALXWn/xxcTDLLgD0KCs4EqSYNAYAsECveKdTgQ8E8Ds4kWAOg 48dIdQzauuimmkWMswjWwZmojjNdQA89FQLUWlWrNmZp3IoQqxsRA1xepRi1a8BfTKc4EJFA6x1Q daRJSLIjbayMW+FiHdQUEVa9FAY83WtWoy2tO65qVHRzryJlFwMP5pr2ql1HnBY47gv0V+FwnhW/ 5byCWC7Cm+E4QFBAs+MYjN9U5pp5hunRhStfnqEmN2lnNUkRBg4I9lLZpDeIvnZ8wscGhzQydB1b woYlDJvvOokSueKtzv45qJlXi5ldakLPPL+Cvbsgrrm+d0Bdu5UADJYvDIKW+fBeFVjhQZEDwnyn kUkYJJHRTA8kFgh/2bFxX35b2YKBSUuUF44QDIhlzRVQQQUAAlaQt6FWEpA4HlAgUtVIAmxA1Yg2 bQ1EFUIt5tFIhhMIoAx9KgWBIlQkrvHAAjFSlUU8aHAQQI1NwrhhQhvcM+WVomSppSDjbOnll2CG KeYECIgw5plopqmmQ+qs6eabcPKxE49xuhkBADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0017_image048.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhawFEAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABo AUAAggAAAAAAAAD/ZoCAgJn//7vg48DAwP///wP/eLrc/jDKSRu4OOvNu/9VKI4k9J1o6pVs6z5q LM80cNS4fOd8r+6+YAYoLAoVxqSPqMwxm9DUM6qjWqVTauCKyXJB3/BQHPMmt2Sb2RdouwODtxu6 Tl/q9mP+hK/J4X9oVEhKbgOHiImKc0Z9ZI57TpErRm+Kl4iMTYRFbZifl21FkGGkkzOmXKkcnqCu cYKNq6xwr7awQbODp1G6vUG1t66iskLBwraxOL50vM3OYzytyK/KPZzS1MjEy9DR3qPgd9nat9zX vtPl5pLizOJd8Kvq69W5usflBMLWP/LwHgQIFCDlH4182hB+6odqlkJhBCJGZEfjnRKLXAYORIGx /9HBerAMJUOH4+EtiSgpVnEHcINGjXwMyjBZ7Y/KGthmgkQp8aY/lh94Ch1KtKhRoS83ggEag2ZN OT4b+gG1z1XRqDGZtsSQlKPMFCbpLQrk9BBDLB8x8bQqdNtKcB2tJCW4FG7ThZZAkeW3zJTCoVTb 8i3zdSuArlntqlAYSK9NtxVJPQQcuCe1sxvi6jHs8qdiFA/34hUJWepdtURfVS2H+Zs3zVRgetX6 IXTjYfVac8h5wmRqkMM8v+bM9eXsz7UdPwa+MPLpT5SZNy9Iu+VcupSqs1KeV/r0Mo6crvWulzpy DkfTq19P4Hri4b2fiiWfyTTYk967LzIPn/jc9//Q1FFWHPQFp0N4BQ5TwILz6WZDYVv9V1d/HgxI ngEYYoiJg0P0YaF0ASxYQIgjbngchYYhNmGA8SUISoYwmgjeYi4uxKCIjJ3IInEZYAegM2t8KB2M Gcr4g4feWWYjiSPm+CMvsEUh25N2EBFkfvMlQqSGoRxII3PjbchkiE6uuAcnUTahYnanLDBOcsxB 9eKWRqJ1H3DRhUJmiXV2kGZDSPyZhIRsTuJAiyDd9gmR39kJGp55ihkWRy9UOkFQ7GWqqUTugWDp pzDAmaiimBTZKKVforaaqoLlpuOZhAhqBKF+AhkronrSRJaQXc545yVhsqqkq1SmYSWPh625G5D/ rm0nKWm56rcOh3cgCWyr1wY77atQIpusUmZ2K2oipI4lZ6Je/prIb8KCyG2b3qICoQaMlUuutNv6 +mi22tZo1ruGemtcsZFcGS2+/lKrRqrrRupvHAAXjKyyy2qXQb3LPfyvvrgi4vDDusmK08QUayBy RR2blbHGEHOc8gD9gszfjh1sajN7nRZKc4Wj8Qqcwgsz7O+wp9ZqMTy0VnyesxpDWzQfCD6MbZ9G L23dwOHC+3KB5z7tqU5DT02ucDtzNmXWAe9bo7312adugez2GvGZ8b6FYgc+88P2xuk+hwzRisQ9 NtnMkgyuzoWrvU2WKq88eN9C2xKzx2LzPXce/ycHUbLJ8zKtN8KNgw50tWmdVPkhHyucud0pbh7P 0Rt8uHdICI9O+gwffkw54JZfXiWmNwdfVM5V383z512bm6XtQeNuuu5YoS0xj0ln1jneyIMevaPO S3769oiL6x/W4WvtN3dOX8YDb+cLO1G+8sIuztnlpw32MI4P5oRDZU0+0sjyQxrhxHc//DHuf/vr Qd7U140AQsN1r7Pa21x0jr6wQWbtkCDSIPggB2IvYUd4xwLtsb7rzY+DqyNMDkZooHsYg4VjWYIJ ASC8GiKlepzzYIVgeK9idAKGFcyg8QCCw2YlThpAZN7XzpC3IJZQh6fgYAc1eJAD4oUOUdJe6Fou MkNw0K94ZQOGFd8wCFntyonhgGK8UjjAF+7qC+yrGxiPKEfp0a2O3MPjHAmoR6UNsY9dYGMpuohH QfqukIZUBSHrmEg79hFUkIykm9whyUpWSh6WzGQLEgAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0057.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Experiment
Control
reverse transcription
hybridization
isolate + identify
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0057_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAADPM/3Bub4CAgIKBgpKRkp+fn5n//6urq7a2ttLS0sDAwMrJydva2v9QUP9mAPj4 +OLi4vHx8erq6v///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIEJo2LkJGSQASVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbAIdGlQn0aNTiCFdesUn 06dBlGqpQLWq1atYs2rdyrWr16tWvoodS7asVpKN0m6pEIdtFbdv4ILE5JSJ3DZ3o+RdsxfqkL5p ADcRfIawRJNXDJdRnITxGMcI1dZdAjlM5SKXv2QGKHlt27CfR6adTDk0lc1dUPulIViA69ewY8ue Tbu27dgrVAPRPXU1Et5ZgPcQntj3EeKg35r+iIr08eVSkCsXja14itvYs2u3nTt5XLTNsUg/7d3N +HmjPVr/Pp39XIfOjZyPXh7vyW7BVWzfzx979/bmoVVdfWzMZ4OBUCCoDv87ANrXYIEClhAfEa31 Z+GFrv1HHhwKLuiMeNDpFeJG+IHIIYF8RUjChH+N+ESHg6moHoojQABBADfiiOONOfLIowg/6mij jkIG6SORRx6pAowxMFkadRKa6N4UTipRZTglrhfggykah5mLTlz5232IcTnCAw8EkKaaaqa5pptu ihAnm2iySeeccNqZZ55L0hhYhOlJueWGU3JkEIstnmjmn1Ca4qifaIjJgqTykSkUfddhqOl+GlIJ ppc8UPrlopGCSuGndqEa0aWE+lBVAgFUsECsVAWQQARCiJoCgq/GOmtVtuK6m6mJ7raAsBXA6hYD yQahKwoKyoqssiIwC+v/D88eJRxVDUjQwLVUFSBCBRMU4JYDDEzgLKRNVtDtt+NWIG6s5Z6b7rDE rqtDBQNUoIBbFaA7rgERuCUBVQfg2yoO/PoLsMCxEmwwwtjKKAKi+ubA1sbjBlBurAEwKwKuBkig sKc7cAywx+KyJXIAJJvsKnhZkgoDVwdYFQAFARzMALPnZWsCcTjrzLPPQM/cKCiYaOngwhCi1HR+ ikLd5XsYj1qoiFWLNrWgT6O8NUbwfG21GkKXkHbFXtd8mllwxy23zm/NbffdX1n8qB9r29H3qmYD 8jcdgy/EKh+Fy5H4QYfvsXjX+UZ5MSGPjx35xYH/Ufmgl0eyedidTy65/+bHfO5PN5nfgVUxpveT NV50B9O666k7Dqwws/PTmSC3yx46Nmrxnnthv0dZO+LGDF98RMrrU+byHUP/6OtLNZ9P46Fbj49D 0qvd/ejfa7/987+Lfw/1T5lvD/aXq4+eUdC7Lw/61X8v+orSyz8/6vnbv3f4/rsfAP23u+Dg7YAI jB0e9Dc/U9BPaZZTXACZdjxMRVBVnWPfizAYtQB+iGoXDOHvpAJCzhFuggMCWwfrwMDsqQABMIyh DGdIwxra8IY4nGGn5tBCF0JOgig8hwqvxsMgnsJtYjMhB0c4oyGOIIdQjKIUc9inExpxep75oQil R74kgm6LV7SZGXpIAv8ybq+JTivBFNfIxihWcYnL+9AD9wVHMZjxjF4w4x3vOL45DqeOYODj+dAI qTYa8pAyfOMXr6jBMAEyNWEckB9T9kguCPJ6FeQaGAMZyTGqoEc7QlIoQTkkUgJplKcsZSqNRCRF MqqT+APf2V5JS1hiDokWVOJibOmILuZykY/hpSSziII3tUlPxzRmnZQpp2Q2c5nPxJOdXEmGS7qO kGLcJfGEOczeaJGT3DyBLzWpS82Ec5soQKQ620hNc55TQnSxZCUT9E7jOZCY5cRnPe8ZvBLuyyoJ kBWt2HKrUwXSmgMJXiY3qLSAxopaIWtWrsC5zxROko45CCjPQNYxcpn/SwToUtdE81hRccqSXS+I wLw4CrIKSEwEB6tAwjKmz5IKcKGOzAGuVmYrjr0sZiOdCkIZAr800uBfDRgoVQJaq6NV4Ge1OllY hnqYfjqxlrO0qTgvqrF55oqqJKopEfUCVl7qkUplFeZZE5TWsKYmgXDF24vaGlarCs5vdDXUPQfB x95p1QZF5RuHovrXHDTSbwXya2ENG1jkjUB5ClwsD0gYCIARVqiRlew1+BrXzoJFs0f4YGWVClpC cPUxOrtsaQExTtVldrUhwSbiVAtb1qrntJakbW39gDrcynO3g/CtHYFL3OIa97jIbccshJvccnSG uc19Bz+jSx3uUXcu/7G8Lta0W93/cZciBfzuRQIl3vKatySyPS/jGqvehApQPJ6Nb1nauwToymCt 9K0Ie8kJzPz2YL/8XaF/KbFXfwp4wD4Ir1HRhuBr4DSnm2wwYMXKYAn/gH9XFcE6N+zGXVn4wun9 5RvhQuJKtSCvFnZMiaPH0mLt8MOGDXGAPcziFRt0UjD+bz3s6wLCbOrH2WlnjmvAY9Z4dci9LPLN jozkGSm5x0xuMha9mQIOW/mGQpayODBH4RPY2MYuzrKW8zm0x5qZxWGm8ZhpQNkFe/nMLGUSY1Dc PQWzC8h4ps2L1ywOu6JUm3wGYoQDHcxvErrCVb6yomO450OvoLUMPf8xnMEc1EY7Wojv/XONJ21i HF/aBYdNFZQ3jeZKi/nT2X2ypPWT51a/xtKohqeqVz3oWHe5VLZWQaifVGtCY9jNGl70omGda4XM Glov+DKc04zsXG8Z2N4jdYtNrWZns+DBjVmBq7dN7OdY28iGboxua8ugrBZGvnIzbm8zjM65jnu1 bWYXKX+kyiKJst5JSuUq7x2kaoeJzuC9dbTjvGxqN/vbssY2rz1N8FLT9NS2Pvaoaa1srXU74gpf +Al6VAFV2qjj+543v+8d8pJDPNZ2zmY1EQ4876q80Cz3SMatFGUpS5zh6RS2oi+O6pSbu4ycdrhU /c1yn3txxEG/Mc//Pw1gek684ko/ec/dmalt53npl941zcNd7KZDmMzFzrSmYR7zk758uGW/pdc1 fmCEaz3bXE87tD0pd4oW05l3QiY0oZl3ZUqT79KUet1FPNaY3xzrlqm70QmP6LIvfsaNNzykv15G gAoUWAUdQQE2+til+mqgwVJbXmhrsnEDHHqHt7RbHNqsZUk0AA1QwNCONS6IWmvgQD847g0vY6eL QKOb9qi9REqrd4FLXuOqF0jvVUYHuNQAT42VBJjq/GQ5PwA5233R1y7qka20xC4tGEwptvmG/Wtc EAv/xGRaxn8ZIAGzitX5LXv+CMg+92mPN4B2Gj3WR3RkAVAysDd//xzFFh/jMm4BVGZGLhJgAOOy ABxDK+cnAcQndI6HT0ilVMkCLE4FVeRSNBzYM0+VNBooMz3FVDEVML2Cf47HfWw3BQ5wLeCWf1RH BRMQAQxwA6eHejMHd70WaG83JnGHcrcFX+h2hFyheL82eMWQekz4hFAYhVTghFIIB0tYhbYQhFi4 CVq4hZoweV4YhvtUbmK4CutWhrHggmiYCV24honQhm54CHAYh4YAhnR4hyhkh3jICr23h5Ewh34Y W2IXiF/Yg4R4iPmih4gYXH24iHVYhI4oCWcYiZBAhZR4iZgoYZaYiU1hiJx4B574ibfgcqJYh5tY ilOIim8YiqoYB3qP14p78IqwiF6sOIu2eBiDeIuxWGC6GBJ+1ovACCWnGIxFIIvE6Iq/eIzKOBGK uIxoYIzOuAbQGI0twTTUeI0QQYbYyAaTuI1uMIzeOFm5GI69wIvk+I3JeI7quI7s2I7u+I7wGI/y OI/0WI/2eI/4mI/6uI/8OAYhAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0057_image049.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhxQAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAC6 ABEAgwAAAHh3eGdmZoaGhp6dnZOSkrGwsLm5uaenp8HBwdfW193d3cnIydDQ0OPj4+rq6gT/EMhJ qwzJ2c27B4ryjQCmkVeGhpY5sagHx5PhOIfwfM6z08DNLDjp/VDGlaiSlAyJzmXwkQAMeiPfRge1 cLsdLU3cJXe/YMmRcAy3J+w0/C1X0z9mYp4Yrz8GE3cVewJYcoWCaXt4iSiLNIh1AD4Ig2I+Pz4B Cw8HTpg7AQcOAgsiAgw+CgUTBZwKASCgJSoGmA0SDT6ABD0NsRZaCQ8LAbY+uAC6f5MPm50Xo6Ui LhIDnGw/1c2gGqiqrAqz2wAF4gustBrDDoAZDwqAgUeXD6ypBnZFPgQECwCcAgx4cOJarAP/mu3T piChEwHlnAUgtoFSxH8I4FFYALHZvQf5/4z0+9ckgAMGADL+aDKpowIHsQIOPEGG5cBKulgZqTTQ 4TBo+oKKmZjQjI+OIIr+eCnBRtCnBahYqyRrVjB6P3QRkEAgX1CidpAqBDAMGJk94rYmFdqGTCoJ UZecbXMN6Nyn9SjsISAuEx2jbThJQPmUw10rFEHoxVp4rKGxkCUMowqnb+HDY+8ineh3Hl6abdts ilcPmOfTTe0hUNvMdEXGuUAOqIKaYOO8eNvYon3B1MDOtWHfpTwJ9OmhUiObETxWHMpCl9voaED4 0/MTllDPZCAWOe08YjgB4oyajRQJzHE/FZfuAeG7DqgO88qJlUqSD7YyICWhR4BkgAXAWaUs2RBE m38AvpGKPFyBwttiqMmSzD768RfZWBmJEFViQzkgBReaDAgAgk8NJEJG8hzmDzFeWdFDFQ4kEIsA PyWAlHmxYOIQG8U0JI8NyQGAIzdtFDBhDT1Ut5gWVgnJDAU0+mCjHYkR2dQNJslopRGg7MBjAD46 CYuVcL3SHiZkSqLmmjRswuabcMYpJ5sGsDbnnXjmmWcP2Onp55+AgvESg4ECGgEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0057_image050.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhPgACAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQA8 AAEAgAAAAP9QUAINTHCGsHqdWIpOoggXLAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0057_image051.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVgAxAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABT AC0AgQAAAP9QUP///wECAwKdlI+py+0PFZi02ouz3rxT44XiSF5giabqdK7uu7XwTMv0vdr4Tur8 3/EBhxgh8cgSIJcmJfMJMEJ50imuaq05s0Qs9+X95rZiKrl8PaO1m0AA8IbD33E6fXKXu+X6vJ3/ JxK21qNGCGZ4OKZ4M8gYlPhY4iipQVlZFIkZcrlZ0emZFJoCGlrqebqZirla2Sr5+hjLOKsYcYub q9tQAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Driver (control)
Tester (experiment)
=
reverse transcription
+ adapter A ligation
=
reverse transcription
+ adapter B ligation
reverse transcription
denature + hybridize
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAADPM/3Bub4CAgIKBgpKRkp+fn5n//6urq7a2ttLS0sDAwMrJydva2v8AAP9QUPj4 +OLi4vHx8erq6v///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIEJo2LkJGSQASVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbAIdGlQn0aNTiCFdesUn 06dBlEI9UqGq1atYs2rdyrWr169YhTUaO5WqnApiyZYtgjZO27UD376Ru8skXCB02+StRdbpXRp7 1wSO1fevkMFpEL8a69ewDMVnIDtup1iA5cuYM2vezLmz58wrJE9OJ5pM6dHkTotR3bBEY9QqWIOR vYkdbBuVP+vezbtz6GCYSLy+bYK2F+OZGHskjvsscIfDmZNAzoX6pG7Sm6vozb277t/AbGeHYV1L eUjix7s4j4U9K2zqY+T2Tr++APDhncWX7/y50f0suGeFgIikB2AKBFKRoP8hBh54woJSQEhIgw6W MJ99GO4WYFr/Vfhgfx5+IyEUIw7SYYjFrQCBCCtC0GIALsLIIowvyhjjijbWqCONMubYY2zAiaAc igiCWJdH0Y1XohNLAqJQktI1yYSUIQVnCpEfbpfhlt8BiWU2VCoR5peKjImEmbcsR2aKQViVQAAV LABnVQEkEMFha1YzYpx3wvlmWwxU8CZeeVLDXlUNSNDAoFUVIEIFExTQlgMMTNBmodG4V8EAFSjQ VgWUPmpABG1JUNUBhIYHX6Gawumqq5G6GqgIdxogQaq8dGNlnodydcBVAVAQgKkMBJogmiUJt+ua yLJlzLKsGqmLXZg2S4T/tSlBy6u0tsCjLZbY4pkfdtWCZe656Ka7VZCuYZoEsANR6+61n4a7mJrz siUXnf4IBYe9qeXFLz/+zrXTwBb+I6/BMNGJMJsADxWxNfDGZlU+FKoxsZ4Xh9bxPbpC2cXGmYaF YMX2iDwGydCgbPHD8izsBsvP0EbzIoXVcXO+LZmicnUu83zvt3oFLTRTHx9NGNFF76x0Tfhy+3Qq Bc88NWFC/nz1SFpvjdGJXuPUddhfR032Tvqd/dPYakuUcdskhQz3TWzPzVDddhOUc94z7c33S2D/ zbXZgscdeOEbSYU4THgvvpa6kEcuudGOQ+K0dpWL0fiGbmXeha5TSu05/xVVG3F5Dac7XrqznY+u hcymi+56IanPUDviq1+7AgK89+7778AHL/zwxP+O3+xV6Lf58VYjH9QUt/Nn3uTUVw9W30zH3noW 0b/Q/ZFCPqFY8eSXbz7xzA+4/Uq5D/H9elu8zzlL7Yv7b/wjPKD//g9wwT//I5Bft7LHuvuZJ3// e8D5FshA8iVQfwGs1u4aSMEK8i59VXjLA/23wUcxblXvkl2E1ocSuS1BgBhUEAL/x8EERhBwsCsg CixIQwamEHor5F8Nd1i+B/bPg4Aj3JlEGAUNunALPnwh/YRoFhKqz4BLDB+TiEiiHO6vhSwEIgyX Bz8V8PCLw7vhCEXwQP8wmvF3SdQis6goPitCEIkdfBX7MMHFFqDQS9xzIkgKU0cxomFnRsyiFtIo x5PwsY16VCEZE3jGRiKAkHdchVoI6L4JOhKMfqziIgVpmiAyUXtQbI8bfzjIOEYyIe2aYiJxuEkd XhKMkIxi1hAZyisEEoBwPGIhDRlDGZ7glZjEoyhrWUK1NOGUWcpjK6+YS0EisyB9FOYOsJKAOM0J LXbS3QGXqT8ctWhHN5qRN3k0znD6qJwvCmcs6UbLHrSlmn56VaAGdaltBiCOWVjntnRQTWHJES2Q kpQIKGUp+w2TYfvMQQQcpUWAjqpUpzKoLUeJRVzusoRjVOir3gnQAMz/KgC1ulU9lXlPRgKTh/pk n8/aeQNPNeCaVakmnYRFLGON9KD4xEJKMWrM0K0yo82jXy8r+dMiFjVxn/SlXvBHTMMN9aZBPWhU MXolVTZVkTygpjUvlk2i0iSaJ7OeWMdKOVa6UwTwFJQ81SpRXj7VDM+cJj8r4M+3ADRSk6qUV1WK h7jq4DwLVaJDSSUCU1UAVVAtJlhZOgjAbhStHf1oSNsat6RqDBHncSlMBXUxmlagWDCTqyevtFgx YfaoXwMdavfgVzVS9Q6txUFsYyux017Vc7RF3WoLl1vAkPW31ttiVenQW+ex4JA6M9SBkEtc5QJo kqU9pnOfKxzYZspD/2/943Ud5Lc5FNee1O2pd7e7XFBEl7HG+C6Z1DtR7lqWDex94nIPN9X0Vkhx dogvVrGbB/2a9bn0ra8w/JuNAAs4GAS2RneTu4wEV2PBDD6Gg6kxJD1M2KchOq9RJXxfcvVVGRem x3tva4sQe8jETSyvFPmAYqWqR3msbfFe96Ph9vLCZACu8X5LnLT41I+Nqejxi0c83hvL+BgOEcSR fbDkCoXWuDFZcSCa3DYI94HKaqMj7aDMkSdzmSFe/jJCwixmzVGSxVie2o8tXOaeSdkQZG7zFtYc YzmXIbtotnNE4qxn0hH5D3zuczM8vIg0u+tJkgi0oJMyCUUvWho6Xv/Zo8dsaCy9LRGOnnRUVMuJ TGvaB/glhac/DWpYjJrUNkjbK06NahlEWmesbrUL8KwJIcs6B1aOha1vTYNc16KsvG5BhSW861j3 +dVKNhmwW+3rBmcl2O0SL8V2fWsDExva58A2yP6sbXpYu9siHi641YHscbOC0ObWBp3Tze52u/vd yCs3vDOy0nkruN72pjCt880Xfqub2/6mhaoDroxQE5wZ8j54Sb6t8Hs13NsJf7gd1i1xUiC64rni NMa7tfGOe/zjUQa5wNEtcqoBvOSRoDjK0XPmlbscRZd+OctJLnNNMLzmiVA5zid08p3z/M0+r03L g0502Oy76E7qOdKv/aDzpfOh6U7fw9GjTnWiTL3qKVE61u9w8a0zSONeN1HYx072g0e87EkZOtrr oPa1Z/3sbodC2+NOB7jTfQlXv3sv8K33p/O972maO+AHDxCDE57tfz/8xKWt+MYjxPCOh0OzI88G LVP+8noTPOZ7kvjNV77znt+75kNPegqDsPTDADvq77x6yQO99cgAPexzIu7Z2/72uM+97nfP+977 /vfAD77wh0/84hv/+C4PAQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image052.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhJgEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAAX AREAgwAAAHh3eGdmZoaGhp6dnZOSkrGwsLm5uaenp9fW18HBwd3d3dDQ0MnIyePj4+rq6gT/EMhJ q704653S/qAVKE5oft2JjWXImmn1SrFq1arhOIfw2MDgBCdUOR6/oo0IPCZBTpinEqVNlczQQwEY HJXgsPiCvPjE5/GkbGIX3cI0+El4qu/4tn1SF/fxcB+BQIM2f3MDa3mLjBmDAl9gkHtihY6UNpYm k5UPCBVISaEAAQcOAQsPBxKlp6mrrKYCC1MERwwBrKkGSagPvKwkFAUJDwsFwSUKDw6JIruibKMA SL6wxaGts1MCDUgJyBIFqQm52GUsvEgMEgxIzrYPuGQ/ywsB6vLt79WvE9q0SAmTMCBVnV4DqYVC UqLbN2TnfswAQMxYOIHKmCUi8SCBMwlu/8qEQubNgMIHJH9JcEKAwAKKDwIEMCbhHqkkNmeudDPA EwB3yJx86vnSQs4nIaOlNKlw54OWRVMF6NmiYK4DRd1ECZCgKA0B4mLO9EoByacCNBF0pLAALJKl TqECqBLAQQMAapNUoQYWQIJTAKRSBfmkSs9PQJ0OpSlhmao1SKOxYsxmLOS+fheKuluWc9kn3sJO SUrGM+TTTW9mjYzZ72oaLXQQ/hyWS5dPNDTTQ+2OgAQCTCszVtiazbJcqZMPeUrjtXI2oWFeIT2h 4OPk0iLP3v6cEjlYgTuCx66d/IXvtLdTp16BADbuqLmnkmBaE3c2RGnEZ789knkKy+DGx/973d1X XlOYzSSZeuWxB0c1FnhzHQASgufggspRUOF+GDJjoAy09BQNcvElx0sBCPgGGYm7ceeOAQPYhl0L GWbHIQW8yEhKiDaiduFpAlLTwnpJ+MAZG0aWWMxdkFAQox1PxldMOA8ceaAFUX6Y2ky28RfedksC 0GSBEvjAgGmZMUljeslR1QBmwskYCBupJKJgfHVcUdOCDyYxJUiekeYAbsswlQoyeb1kFoUPOLOo Nx/BcRASXeYyC0iWesXTWmo5euUamU5wKF5IvHSEbw040NcRAbAzp1gQTuqAbayyoxyk7S2k443L 2boTqqqWmJpaHqBFWS8OXHFGNQrmUuuudj150CmvLhnDVBdHcDFrLkjwsoCKCnkLbihk6VDlGi7Z RE26yC00wTgdUTmKu6CwW122cylgqWMKYJangsMBUMc9XTljroz/nvREAb7ieASahIk0jcCNUiAA v3CWChkbOpyyrcJzaVbGwFwtkEjC9MILzsY/0HtCIfY1IvPMNG+ASs04d9Jizjz3XLMB4PosNAgw YzL00UifcMSaSTcd8R4uOy311BT89RHVeEQAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image053.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVgAxAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABT AC4AhAAAAADM/3Bub4KBgpKRkp+fn6urq7a2ttLS0sDAwMrJydva2vj4+OLi4vHx8erq6v///wEC AwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwXfICSOZGmeaKqubAm8 cCzPdG3feB5Deu//QBovSCwaYcOjcolLMp9QgDNKNU6r2N81y212v7otePwSk8HmczetzrLb1Tc8 Kp8/6/YlPn/c84t+f0GBglqFXISHYYpYiYw3jo81kZIzbAGYmZqbnJ2en6CbXpV3pKWmeqipqn1k IwZSCFIiAAYMcWcQCLdSsEMJELB0jRAKDQrCIgNlDgNDCwkOVJQ2EAIQB0MQ0GUEDEMNIgXDjVLm 5s3mwC+3BA3k0ykFJAAPAOEJwNRIrK39gP8ABhw0cFCLgwgTKkQRAgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image054.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVgBPAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABU AEoAhAAAAACZmYKBgpKRkp+fn5n//6urq7a2ttLS0sDAwNva2srJyf9QUPj4+Orq6vHx8eLi4v// /wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/YCSOZGmeaKqu7Am8 cCzPdG3feB6LcOD/Pp1wSHwBf7tI7+grOJ/QqHRKrVqjzEByySx6v7PsFkwue3lGsXmdU7/QgGzg Sq/brW4AXM7u2/JwfoJre3mDfoBKaUx3jY50iVxHh4eRlJdninFZj52eT5abXZh9oaSnN4WjqGWh cp+wjq6GrF6mtbh6mny5X7NMDADBwsLBw8bGL8jExMfFzMvODMnEv5O9tqu62Lmq19xD1kCx5HXi SODh2oHpmOztlO/wg/LzfvX2bPj5ZvtkJQYiINClxEADVP7+vQiox4CeFwkiOCSVcEhAB29gKInw QIAiBQkenKoopIEAjSgjhQxooAiCCAIUNa05qEgRQyURXxwcACGmnwMRFhAUEZAHRpcJIsqkt5Rf H5JOiUCNKmQq1RwtsmrdylVFr6ZXy4ANC2Ys2TNn9aUltLZfW7FvFcb9YnZuKrto8UrVu5dvVb9/ AWMVPJjwXcM26hpWTJixYMeAIes1gVgGicqWR2DOvJlzrhAAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image055.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSQAHAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image056.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSQAHAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAABD AAMAgQAAAACZmf9QUAECAwIejG+gy+0Po2QICXVFBppjvnndJYYm6JVcdUzuC0cFADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image057.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhUQAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image058.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhUQAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAABL AAYAgQAAAACZmf9QUAECAwI2jG+gy+0Po5wAIaGw0Bls73Hgh42ixpGd+lgHBcfyTNf25AYRaoJ9 yQOGfKehkCXI3ZbMRq4AADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image059.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhUQAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image060.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhUQAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAABL AAcAgQAAAACZmZn//wECAwInjG+gy+0Po5wAIYGz3rz7D26PdVDmiabqyrbuC5MBFNb2Dcrw7soF ADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image061.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSQAJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image062.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSQAJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAABE AAYAgQAAAACZmZn//wECAwIgjG+gy+0Po2wIiYuz3rz7r1XHRJbmiabqyrauA8by/BUAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image063.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhQQAJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image064.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhQQAJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAAA8 AAUAgAAAAJn//wIXjI+py+0Jopy02ouz3rz7D4bYQ5amUwAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0049_image065.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhPgACAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAAA8 AAEAgAAAAJn//wIGjI+py+1ZADt= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0050.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Mix tester populations
hybridize and fill up overhang
PCR enrichment
<= /td>
isolate +
characterize
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0050_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBgwAAAAAAAACZmYCAgJn///8AAP8zAP9QUP///wECAwECAwECAwECAwECAwECAwECAwT/MMhJ q7046827/2AojmRpnmiqrmzrvnAsz3Rt33iu73zv/8CgcEgsGo/IpHLJbDqf0Kh0Sq1ar9isdsvt er/gsHhMLpvP6LR6zW673/C4fE6v2+/4vF42sPT3gIGCMAOFhoWDiYqLIX9/AY+MkpOUkBORlZma eo+Ym5+gcYahpKVqnp6mqqtah4essLFXjrK1tlapt7q7Qa68v8A/ucHExXx+xsnKKq6jy8/QIKjR 1NUY09bZ1tja3dCRw97iwK/j5ufo6err7O3u71TO8POgnfT3mvb4+5KO4fwAAwocSEMewYN19CFc KOoSw4dvFEKcmMYgxYto/mHcmKUcx49d/zSCHDlFIsmTJT2iXAlFJMuXRkzCnBlTJc2bQ2jh3HnE Jc+fMXQCHaqjj1GiSHEg8pm0qdOnSSxCnXriKFOqWC9Yzcp1hNGrXcOKHUu2rNmzMzCBRUuUG9uw iCisfcvzq0O6cP3NxbuzGV+4yP5mdSsYKuHCT/0ixqp4seHAjp0ejjx0MmWgjS9r3kzRJmfMEux+ ruxw72iM+kyf7hzX0urXsGPLJiVztu3buHPr3p3DMm/WmX9vBNdaOMjixjlKTf5xOfOJxFU/d9cM +XSGvq8TdK59Ye3uCL+D3353PETu5tOrX5N9/bzq0t13iy8fHfr69LbiF6h/P0DR/gUoIP8W9w24 jngGHlhegvD4wyA/9D2ojGcSqhNhhRMuiKGFFG5ozoUeEtNeiNTAR+I5IJ74S4oq3tJfi+K8CON8 Bc74DII23thhjt+ExiOKPwYp5JDtsEhkJtUduQyOSvLCZJO71AjllP49SaUsvrh25Ypb6mhkl4pY CaYqYo5ZSplm0mZdmmyCN2Kbn5gIZyw7zmkKmnZSgmee/WjIJ5lr/inobKN8OSgcWxl6aBuJLkqb a4E6qqdcktZTgaKVZiQXppmeUWen/RwFaj2cjqqpqaimqqoHpa4ahoyuCgJrrIB8Susde97KqK26 ziFUr4m0CiwuvA4bkbHBRoqsHLkuW0b/as4mlGS0vlJr7bXYZgtEocAg0GWjvHi7Jbi7iHulqMqy Yu65lIZrppSmrMuuqOWOOass8lIJYL3a0pHvlM1m8i+UWfL7rY+/DMyuu2AWrIvCShYbr72WCFsH xEfeGwvGRO77cJsW+2tvcPhSjLDB/cbB8ZABV7KykFmGDMfLQcpsB801p6uuyVp+3LCfsCAg9NAH Q2LzG0MLnTLSRBd99MxKH0zvLVE3PDXVS3OQ9NZcd+3112CHLfbYZJdt9tloiy0hzt6wbZ7b2sCd mM48yG2N3Um1DAPe1PDdFtBA+A2N4KCdTAThyyD+E7w5mFvA45A/vkTkkX8gwOWYC8BE/+aZX6C4 cI5T/vgBARxAOumlp5666aqffroErrde+uusox677aJL7gHnl9cO++yy++776q8THzztxbvO++We 06X3C6GLPnnulvO++fLNvzXtENFTPr30u1u/xPKaW/A5TtByP0HuBQx/O/C2w/+7+/LTbz/71XN+ /P7vGx+//cnjH/myV5inmW991FMC/sKnv/Fh74AFNGAFzlcMCu7GgsHAIEzedAMNJsx52wuCBxnG ll8d7h4jnIkECbjAJLSQAw9UwgAhWEKJ4aB7kSOADnfIwx768IdADKIQefjCDSxviEhMohKFOMMJ Om8JOITc97zHwM45UHxOBOEKCUiFBP+CoYkUSCFKNOaDKOpOgeDrQAyTAMYJiPEkZOyBGQuwxDra EYlehCHv7sjHPu6wjRJ4I0k8pj4JFLELa7yCIAephEWWTHsVO4IjN/bEqwlhkkHT4hZpCA9MDgeK KHyi4Qo5D0+ixoYdDCUke3ZCepiyOZJUZQ0dJkJZouV5LnjlxB5kLgP48pe+FEMiraBLzfQSmL/0 ozLtaIIjLvOZSPxAMS9zTGQaQJhYxMI0z9NIW54ljnWbgDWDGYZhVmGb2ImkEaqJTGi6M4jN3OM7 59lDD6DTO4y7oTjHic0GZuGe4QGlK0XJykt60yy0NOhAV7nJLJZSMA1140HLktBaLrT/hAJ9qBaR AFBNdJQ8o1SoRjGKsIZijJ4oJUALUkpPaWoSlTP4qMsIytGJBkimlMApXyDG0payoKfztOdiTGrT Deg0RiQrY1E10DTShNSiIwXB1pxaUKh2Mm1cqwzddnBURsAtadPp6iK+KtZtQMaq7yjrPeQk0k5q sqYXvSXgfqDWQdT1PXNValy/mVc5LlUs+UzlXgd010AUtkFb1cFh97DYIvU1nIOl6GO5+teu4LIF jc1DZtkRWBts9g6fzUpoL4aXztZgtCJbZVUDV1mupK+taX2paffW2sFk1K28rO16UDsH3qYjooHU 7WASq8/IkoW4ihUuY1ZLV+UyBrgB8PCtyiAaS+MeF7mCjSpfLYlWd0jXG+CkrHUBazS4+oB87wMg /+aHvPX6D3aH1CuDdAnIIsTXrww1L24xyl3W/qC+RLgvZLXX3+b+d3nq7Z+C5ffeBM9PwOKdLxAA PAQIJze/1dUuQtVZhO8irbQFlu9+E+RhN5RYG7ONqXMTw+FWaliyIcbvi49b3gyP2EAnZkOOq8FB z65YMiH074zHAt0w/rgpJiTljTcc4wEvGaFNjvCTb3pk7exYDVf+SZbRsOWddNkMX75JmMkw5pmU WQxnfglW18zmNrv5zXAuW9bmTOc62/nOeM6znvfM5xZFAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0050_image066.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhJgEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAAX AREAgwAAAHh3eGdmZoaGhp6dnZOSkrGwsLm5uaenp9fW18HBwd3d3dDQ0MnIyePj4+rq6gT/EMhJ q704653S/qAVKE5oft2JjWXImmn1SrFq1arhOIfw2MDgBCdUOR6/oo0IPCZBTpinEqVNlczQQwEY HJXgsPiCvPjE5/GkbGIX3cI0+El4qu/4tn1SF/fxcB+BQIM2f3MDa3mLjBmDAl9gkHtihY6UNpYm k5UPCBVISaEAAQcOAQsPBxKlp6mrrKYCC1MERwwBrKkGSagPvKwkFAUJDwsFwSUKDw6JIruibKMA SL6wxaGts1MCDUgJyBIFqQm52GUsvEgMEgxIzrYPuGQ/ywsB6vLt79WvE9q0SAmTMCBVnV4DqYVC UqLbN2TnfswAQMxYOIHKmCUi8SCBMwlu/8qEQubNgMIHJH9JcEKAwAKKDwIEMCbhHqkkNmeudDPA EwB3yJx86vnSQs4nIaOlNKlw54OWRVMF6NmiYK4DRd1ECZCgKA0B4mLO9EoByacCNBF0pLAALJKl TqECqBLAQQMAapNUoQYWQIJTAKRSBfmkSs9PQJ0OpSlhmao1SKOxYsxmLOS+fheKuluWc9kn3sJO SUrGM+TTTW9mjYzZ72oaLXQQ/hyWS5dPNDTTQ+2OgAQCTCszVtiazbJcqZMPeUrjtXI2oWFeIT2h 4OPk0iLP3v6cEjlYgTuCx66d/IXvtLdTp16BADbuqLmnkmBaE3c2RGnEZ789knkKy+DGx/973d1X XlOYzSSZeuWxB0c1FnhzHQASgufggspRUOF+GDJjoAy09BQNcvElx0sBCPgGGYm7ceeOAQPYhl0L GWbHIQW8yEhKiDaiduFpAlLTwnpJ+MAZG0aWWMxdkFAQox1PxldMOA8ceaAFUX6Y2ky28RfedksC 0GSBEvjAgGmZMUljeslR1QBmwskYCBupJKJgfHVcUdOCDyYxJUiekeYAbsswlQoyeb1kFoUPOLOo Nx/BcRASXeYyC0iWesXTWmo5euUamU5wKF5IvHSEbw040NcRAbAzp1gQTuqAbayyoxyk7S2k443L 2boTqqqWmJpaHqBFWS8OXHFGNQrmUuuudj150CmvLhnDVBdHcDFrLkjwsoCKCnkLbihk6VDlGi7Z RE26yC00wTgdUTmKu6CwW122cylgqWMKYJangsMBUMc9XTljroz/nvREAb7ieASahIk0jcCNUiAA v3CWChkbOpyyrcJzaVbGwFwtkEjC9MILzsY/0HtCIfY1IvPMNG+ASs04d9Jizjz3XLMB4PosNAgw YzL00UifcMSaSTcd8R4uOy311BT89RHVeEQAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0050_image067.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhtAB4AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAACx AHUAggAAAACZmZn///8AAP9QUP///wECAwECAwP/WLrc/jDKSau9OOsHuv9gKI5kaZ5oqq5s+ylu LM90bcvwre9873u5n3BILAILxqRyiUMyn9DoyymtWonBq3Zry3K/YJU3TC6DxuZ0GK1ua9nueBQu ryvp9vwQr+/z+H6BNYCChS6EhokpiIqNJIyOkVOSlCuQlY6XmIqam4adnoKgoX6jpHpBA6qrqqQB r7ABolSsrAQABLe3uLy8ub26uh3BwLjCv7vEyTqxsMvIw8bF0L7R1MrWwtUmqbWtoc2vs6fkpuR1 5udx6ept7O1p7/Bl8vNrVPaR3d6u4eMd3lRpw+br2EBpzw5CuybtRrgAz7JNU6hQYsKJvUrsq9Wv /9m/fPrw6QnY0WMHPAxTGsR4saW1bCRvuNxGsCbClwVZPpR1UmSemJ52HgEpqR7RNz6PflT6KSnT PkafznEq1U7Uqkw2sioJ62PAAQLCih1LtqzZs2jTknUYTq3bt3DVaqQFdNPDpVhRUY1TF5PQnibi Ch7stq8MwogTi/0L4KoSw5QYO85rZDJlLHsvx8usmR7nzvdAW/0smovl0ju0ruIqLpBqVYpjD2bb TLZtuHM9fGXNsxRp1FdOA++S+bZxtEKOK1+be7ga4c5nQI8eYzr1FtavW/qtfUn27ii+g+fGfXwR 8eZH0FnOPmzy9srJp9+Cfv6Z8vZ94IEf/wf/41Xy5VdFfQI2hl+BOhAooIL5MWifg/NBmJ6E5lE4 3nr/GfdehrIFiOATFoIXYncjalfidSdSl2J0KzrX4nAvAhcjajOWVqNoN4K2wY489ujjj0D6CEAC ADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0050_image068.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhcQAmAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABs ACMAggAAAACZmf8AAP9QUP///wECAwECAwECAwPcSLrc/jDKSRu4OOudCf9gKI6k6JXhia5sS6ou Bsd0Xc4xbu+8zOu9IA3IUgWOyIBwiRE4n07fzpg8DgYAbDaL1Xa73O32Kv6Ww2YvFwqV2qhVJpP9 dNeI8jwIj4In9UF0URd8NxhVR2dkaosXaY6KYGOSj2OCAnZDh4iAPZeZOZ2iKTkqhaN5pxwMAKqo SxWxr7OEpSeutD+hoLl6uHu9qL8fw8EuxRvIxivKHcudzbzPQdG106m613LVrdrb2d5C3NzhzlPl 4uDo5+zr7Xfu6prxbz+x9/j5+go8CQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0009.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
RNA isolation
RNA isolation
reverse transcription with modified oligo dT pri= mer
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0009_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAADPM/3Bub4CAgIKBgpKRkp+fn5n/maurq7a2ttLS0sDAwMrJydva2v9QUPj4+OLi 4vHx8erq6v///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIEJo2LkJGSQASVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbFqhQLSo0aNIkypdyrSp 06NXnkqdSrVqUpLOtFCIs7VK1zdfPxLbErZN2Shn16QVS5brULcjx2rFgaCuXbsv7urFsdZJXxh6 77oIjPfGX4mNEretQTjwisZ7axxeMvkx5MIoLmOeURkiJp9NOp/QLDgz6bqS39I4vZkEawSpgaJl zNp0bRqij+Qu8Rr26Ne4T5qMSvu0bePBvRZHbqJ3cpCKQTPZ7fr2b+YyqBPRPsL6dc2xoZuSTvkG eMuXDate3pgFaRvc/yUmX56uY8D31SvPkX9w//Cy+eVee/4RVqCBK8QXxP9k6bXQ4IAIqqBggDr8 hd13kKH34AkT/nDYhby9pwKIJHRIoX4YbhgiiSIAh4KJPVjoXXUsBuAih1iVQJ9uKTiXYoY/Aonj fkES2NyNK7IIYz7sEHlkjUjSWOOSO/Tl45MsRlkiVqjsWISVM0p5XpGRvbgemaUdp6KYa45A5T3z eUTciGP22GaSRprpJJq+0Xknm/+V8KY9mIBS6JwQtpaoohqi1sKgfB3oKH5p5lWmhMJlhehqfwLK aAyQosjepX5G+BxbOmKhYI0tiniqFKuyGuYLobrTJBXxadndrC7UCmAMV+LZaYI5propsLoGC+qZ yPKqLK2Z6rQnpa4KOyz/ptNa6qyuj0Y73BS58mojqyz4+mqzdWJ5bQrmpnMruOatm252zMpQrZqB LhsXNseOam+l8NXL3r+f0ruvsQKr0a7BYHl7aMJpLKxvwyeGZtXFGGds1FAad+zxU94SIvEgIwsn QpyBlJxyxTUY5KUbKgMSs3gn1yzzMTNDd+jLbOTch88swwH0HkMHbRbORssglB9F59F00hEjDTUp UAFT9dSbXM3Lxliv8K4eRRUTdtded8OzwkQZkzbZKsDz8M9Ss83C2388Lbcg396ttw157+13C26f /XfXZgs++NRfH644CX0v7rgpNj8uuZyQTz750pYr3njmnHfud+Kes114/+iDG076iZuj9fHqrHON a+uwf5yjYnMJDTEadjMT3WIU4wqXeHTffkbuIhDPg/E4IV+h8GYob0zqUDgfabYKF1u5qr/Da/vB 1/erFvNlSB+MQ7yfIMD56Kev/vrst+/+++oTSz0J8Ndv//3vs8uS6QtmD6v/HJEW+MhgPPEFjHtc MOCvZrM9mnXvdviLoATrJz/frWCCGMwg+vQXFwHOD3cDHIMCd8G/7QAweie8COieMELOhFAMLbTF 6Lw3Ag3aUIIV1J4KbshD+3HQgQlMoYAaCMTa9U6HMJvdAz84vBeGIYa12J0RT/CABwTAile8ohWx uMUtisCLWaxiFsMIxv8ujtGMZsSWBUvVp3Ftpj+XimOlSLWlDgaPic1zIhigSAvMIfFoeAwfl6A3 RBT08JDuy+H/LojIRqbvh6gqIRD4CK1AEnB/5QPkGpN4unIJMTSf7GTxVCBGLmrxjKc0pSrLmMov tpKMsFTjH83nyFpCciWSrFIo63NEsXQDe0RcZC834kdhcnKTmkTVEpFZglraUpbGVJOY2DTNXb1x jgCrox2DGEwGDlOUgtqlEijZK+vlMkbiTAI5u2XHQp0zB39xpiMV6c0UyLORtyTmF9bpSUuKMFrc /CYL07mQYtYzmdHsmTkz2cx7HpKeKGSkQ3mYT4wYNKICLeQxdfbO4xH/lEfdBKc2N5rQ7yEwch+c aA8hOlCJqlSDFVWh2YCZUVCGVKZ3ZKZJdVo97nV0eTfVKEL1iVIafpGVq0RlKV251DAyFalQZalQ aflSmOrJgT+F50eNwE+pUoSQU93pLMUq0nAG1aY1jcgssqpVe1bVhtA8KFXfOsGYImY8yxxrT3ka NbZIMQtdjStGSapCyOW0pHvVa1+VmVfEioCuVr2qYmsI2bpK1qIIoylhBzvUrzKOraLqbEvT6hmw hjWxjgUhlxorV/pV1rKXbS01rTkpN9ZWjti8Zm1NENhVHFa2i51sE3HJULICV7Vl5e1Wv7RczvU2 tpxVqPWK64OiJCAA/xRYAHbTlgAIVFePkiHKdbO73a1016P7oh1guboA72J3vCJgAAWui16+eqi9 xYNvAORL37YCj7XR5QsFGhCBBtCXKAUongQK0BUHMEAC6PQnqAZc4ANTIMHYXXCDH6zLdgb0eAOg gAK6QgEHF88AEOhKBIhygPoKFz4hHnHxTIxdFKuYxUDFKnXVsxUSB2DB2N1vV7xrgAi4OLU8DnKQ gbwV+YqAyEb2ry9Ny8sqLeUAG5tAAFbMAPkuqYCTvHKWt0yBLq8ttEQFLZqNG2DUnhS8XgDzWRHj QfsO1855fPN651xl0eL0w35G62YLW1SOxe7QGDM0ohc9lYUCuA7P3f+n42ZYt7gdTs0wtHRy6RDp OG/6Dp3uQqg/bVdbjDocay3Eqaf4ObxiWtSa3tvuXg1YrekCKX+j3W9B7bpd2Fpv8BBEr7c2tkvv Gg/FttqZS4dXYa+61ov768rUNmlDkfra2PZpthFX520HSC7eRly4jaapcaOOyubGpHrT/e1ms7vd x373TWgtb33MtN48uSi+983vYui73xx1J8BnQu+BwyneBifqoxOO2UIzvFgIf7jEJ54IcFM8vQu/ eGkzrvGOe/zjIA85QEXecI6TnB//Pnk/Uq5ylEe85TCPORdYLnNC/bLmBFFIwXF+jJ3zXBgv/7nQ h36NzBIdH5Q+OtL/Ha70ffi86a8oN9ThNHX5BL3q2pA21uWh9a23A2VeJ1TYl77usbvL3WZ3F7rT /oyns50TNH/7MqQu929YvO7lcDveWXHzvWedcn4PvOAHT3iV673wGUE74tuu+MXPfe2Oj8XhI98R plN+fN2+PC/urnllTL7zKck86KM4+rZfvfSjiDvqGyKnz6/+DUl/veRlT/va274Mrr99S/qu+1So vvesADzwE3L64Ru/2kY/viZir/xMiL75kfg99PEm/Ok7v/rWnwTks899bue++12QPvj3IP7xlwT7 5kfE9tPP/nwXv/13KD/856Dz+RuC+fYPSf73z//Cf7//ULB+ABgTjf83gAFocgZIfwWYgEywgAx4 BAL4gGjQdRIYf41XgXYAdhi4gYTmgBxICRf4gXLwGSJYggzBeSYIeyGYggSIgCz4guQQgTCoBRQ4 gy2xgjaIDNaWgzzIdfzSg8Nwb0Cog0MYE5ZXhGFQg0iYhGW3hE74hFAYhVI4hVRYhVZ4hViYhVq4 hVzYhV74hV4XAgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0009_image069.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhmAAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABACQ ABEAgwAAAHh3eGdmZp6dnZOSkoaGhqenp7m5ubGwsNfW193d3cHBwdDQ0MnIyerq6uPj4wT/EMhJ Z0rUKKcG0JxXjWRpAtcJBMujsq6UqiRMl06eM4dQzQCCowBoCYmtm/IELD1yqqfDgllOpFZKzgcY PB4BE2M6GWfPArIVqmKfK+5sPPDljuLxN22gXuZJf2+BN3kIDgc4fYN6IwJPZ4sTkX59a5VpChI2 BTo5nJ0SXg4MYSsHDwIKVaKkK0kLDg9ECaA2EgINOQkEkpUTBRt8ZLYBG4aap8WHWmS4uryGOxJj Q39sWBJ4akIBARwSUgMDmdzemVIGAJyZAG7YABsBnDHtvisPDR/XbAph3tk5eOVC0EtCvHkSDDio YtCHNTVutFnoBNGBHVqg6hXUuBEFu4j2/2CVApktH5xh3wDK+KhmjIgBBDlOwNRRoso7inyRlMmz C8aOFB7d5LghAaKaFSkM+ClhnQygAAwtQMqsV6mqVk9utLlC1SeoQ3cCyLUsbNKuCb6WcYCgwNSh 9+jJ5EornyOgdQHcnUuG6wazJDYQ8Xa2KayNaUwS7dtnXgM7W0J9ucqRMBfLoTq95Sksx9udTwIw MNstx0h7CjEIScnGQZhUvdLlInLTNGHKtEb30sEg5u4pGTNGfaJYBzsJCIgDhAKKT4IwGfn0S6CA iHAKycE8WABdR7tx/XoZ6vC7C4cA1GlnVs+ovfv37ifRKAa/vv37bexZQSACv///8Mlnwg8TcgFo 4IFKXGdFArKMEAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0009_image070.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhIQF1AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAd AXIAhQAAABwbRQxuMAuRPQufQgfaWAnJUgnATgjSVQfiWwD/ZgXxYDwKCyEhWSkodCUlaCQjYSoq eicnbjMzmVIJCkwJCl0ICVgICXBub4AAAJaWlpKRkp+fn4KBgqurq9va2sDAwMrJydLS0v9QUOLi 4vHx8f///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwEC AwECAwECAwECAwECAwECAwECAwECAwECAwb/QI1wSCwaj8ikcslsOp/QqHRKrVqvSBN2y+16v+Cw WKwdm8/otHodLrPf8Lh83nXT7/i8/m3f+/+AgU99goWGh3mEiIuMjWSOkJGSVoqTlpeXlZibnIia naChep+ipaZspKeqq2CprK+wVK6xtLVKs0wmZbq7ukK8vW6+v0O8R8YayMnDvsLDRM3Af8rM1c/A 2NnZx9bF1cnehNHKdeXE4Oe7v73Q7UbC58Xp6vHyWveA3+jo9PzE/fj2xeuXbt3Ad+ACesGVix48 fwmX2ZOHsKA7cRTXPcyXEOBBfw73bRRZD6I7gdQeccHncRm8ZhNLfuzjEObMjNMktnwYbN5G/zs8 i9RUGFTmSnPMKBL9Fw5nRoImW0YVpG6nUqYgn56EavWm0S0MlxSFGLIr0Ktk0ZJcyzHi2q4W3dJE GzLu2LCDVibVyBdbR2TahFobx7cpNGPkRsHs5tKZtMbanh0ORtjlZMGVv+C1xZnW5s6gV30OTVrU aCYZUqvWoLq1a9auY8OOTXt17daXZN+2vTvD7N64gSNRQJy4huLGx5xe4js16+fOozeH3rw6dNjY pWennn26JevTtUfnLp48ePDmtxtJfpy9AjPLlaA/v53+ePv204//Xr97f/Hh+QfgdQP6tt57Q7B3 RnzMGTiEcw86KASEE0qIXYREUFhhhhZ+Z/+hhhpeuCGHJGJY4ohCjKCiigoqCN8ZIYL4oYQx0mhj ETJ2UuOJKIrYY448hkhEi+8hpxyMM/Lo444m9rhkh5Mw6SSQTT6J443DIdgegi5qZgZuFUII5m9h OgjmmauVqSYmY6JJoZtmpglnhGKmeYSRW3a5UGl8hsZgn4A68meghB4yaKGItpXooqAcyuijczgK 6aSoUGopJJJeqmkbm3ZaSKaehkqJqKQmUuqpkaKqKh+rtpoGqA+6uiqsdspaaqbAOUhBBRZcsGuv v16gQbDBDsurr8cWS+yxtkoCaq1IMKBBBQxIS6210mKLrQbaTlutt9x6u+0rUBYKq49JVDD/hLpC sNvuukS4K2+88LIi5BECDCBEvpxVMsG/AAcs8MAEZ0AwwNNa0K7CCa/LMK8OLyzxxBCrkusQBBRg QAIZH1CAsskyuyywIofcq7EkpyxspUcc7PLLAWOwwb8y/yvrvUUksK/OGgiwbbfUhnutuOACnS3R QwfNshEwN00wBx+A8AHUIZAwgQMPDIG1EFtznbUGXXcN9tdih03212N7rfXZa6udtttylDsEzxro nMAC79Y7Lb157623u34HDrga/jpt+L9WT4BB4hhMAEEDQzwuhOSTQ64B5ZRfbnnmmG9uueaVR+65 6KGDXjoXhx8+QAIbZ2wAAg1TzHDsFVdM//vstT88+xqFp9601SSUkPgEAUQQAeTFH5888sY3sPzy GjzffPTNS3889cpPbz3z2XfP/edY+G54zYrPbEvv4rtMtQgbkFC1AwFoIAHY8UsAv/z0y3+//fXn zz/++wtg/wQIwPrtz38EnN8BUZe+l0FNalQjwdveVja2TdBsbaMg2jDotgp2cINoc0qzwtdAlzGO caYzHedGl8LOkU6Fn3Nh6VY4wxiC7ysjpEIJTTiB4A0PetvDnvOqpz0iXm97QDTi95LoPSbe8Fyr 2uHB1te+9w3QgFfUHxYLqEUu/u+LCMwiGBN4wAWKMIdVkOLhzofGNlYBimuQmxudtQmczf9xEnD8 knBQhiyV8RFkKgNkH1d2xynkUY9yFNq3knY0RirSaI8EVyENyQk7/k1vguNbJi85SUq2TI2GW0Pu Jna7iJVSdp30JNNAGbAOdAADrnxlByaQSkChj5UkEIEHhKfLElwNhHDjoAcvaMFhGhOYGsxgLZtw S1CCYGYkeGYPHWfD08mQhi1kITa3WU0YvpCBrHwZGz8ZzglIM5rQdKI6lSjEdXqvnUV8JxLjeUMr lPNfroRlPjswzlWWswMkIAEHACrQMm5RgQc1aBcRulCFjvGgYVyo/7pwzx72Mpe7JGYwi4nMY270 oxpNJtzOuMwkVPSc58ymNbXZTW6u9KX/KvXm6UhaUnKGE6XppKc7h3hEds6zp/L0qRKdKJSaOqGi BB1oQDng0KZCNIERfahEoUpGMRbVqEyo6MH6idVCQjGRXe0MrSwZVrGG4WJCGNkg/1iyQLZ1rWVN 1VnJGklHQtJoP0OatOIqh3PRdZOBAyxgATcvvvYVCeU0mMtOabtRojJ2pzQsHJoJyprtk5aSfQVl pfjAD2DUlx79IEhDG1qRinSYmd3sDhl3Tmq+0KUxhS1sZSpTbIJTqwHjKhEqCrwSnHOn8AyqcHlK 3OAuUadCvd5tw2lZWep2CBVdX1Kd6kWqPjWhYqQuQ7/oUIres7OfDSlpO0re0ZZXtCE9/0lmNcBb mqV0ttdsqXxhCl+WfpOmZW1v8H6LXKASF7g/Pa5/d0pU9WY2uh8IgQimm90GT/W6DXXwdrXbXQNL FrcDe+56W3XIK4B1w4HoMBX+CmJFAQKtbPWjWlO81hWX+BaGgNYR8Fo0vdZ1kY3E8YthfIi/Fjaw Px6sJge346v6E8MI44JjG6s7Uzo2skWuyJGZO7Py2SzKjULsdxMsUC7/MoPjBfN5S8vB0yIztVou 5wlp5toavpaFtI3vm+fs5pguF8MaZu89h/fD/gq4uEEMsHEHLWjo3bmyVa5ZnitaRRI0WrtRhXRV qyvhSFv10JzlcgTFO+bzmlnMoEYvamHxG9b2Kg6F9a1znO2rahnWtpsWNqypp/kvAA/Vz8a19XAD Pb1Y85XR7nN0sCWNXUobW6rIpvClSd1VJOe2FiLGsmikjarIWPva2M62trfN7W57+9vgDre4x03u cmsbAEEAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0018.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Reverse transcription
Reverse transcription
amplification with random primer
PAA gel electrophoresis
C
E
<= /td> =
Isolate and characterize differentially
expressed DNA fragments
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0018_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAAAAAmTPM/3Bub4CAgIKBgpKRkp+fn5n/maurq7a2ttLS0sDAwNva2srJyf9QUPj4 +Orq6vHx8eLi4v///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIFJo2LkJGSQAWVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbFqpQLSo0aNIkypdyrSp 06NXnkqdSrVqUpLOtFSIs7VK1zdfPxLbErZN2Shn16QVS5brULcjx2o1MaCu3bt48+rdy7ev37on 1jpZ+7ew4cN9Uwie2KhxWziLmUQ2MzkiJp9NKpPRjISzGM9AhYAGM5pIaS+nAZqMagIChACvYcN+ HZs27dmyZbvObZs37t61f4vobSJ1EMKIkyv/qxirKcyS4VIx/hikY+hLqM/1Kp0tpuputPsQj4V8 6Btnl6tfvzfw2xPs4y9vfv6J+ffTu9fPrh9tf4yoYNfZf/YRSBE73JUg3/+C8xWHn4IMRpgYCvfd 851HrEH2oFnOXSigERXmlyBYWDn0IYgGZpYiY1llSIKEMPrl3oglJGCjjSTciOMIOiaQY48/3hjk jiIAyUKI8yAoIok0chgXNi6GtyEbSMqj5BRVBnCUAhUwoCVRASgQwXFT6rBll19uJaZoJ3WTRZZa isCllgrEGUADFdT5A5w0ZNnVnHnaiaeee7bZYpk2cCmBCF9tVcEEBnTlQAMTAMHnDPcpyugIjkIq KaVkPlnCiab1EIEBnKZawQERdEUBUQgU2uQNp6a66aqtivBqBbHKah2UiNYwZld/OnpnV2MeQIGv WPIw7KZhGounCMkuyyz/R1dKUeECFTyQJlFcgrnoqw3gGeKlMtzHrbdnihsAueZei+2hs6oVLBro 7qeYVfz2669RQ/0r8MBPGXphIPkOknCbIjRGqpPFLOxcw6YgfIzEolb8sL3GYByXhxZ3rC8tHvtR 8sgrkozyDEKZfPHKr5zMh8wwqwEVMDfXzEnOvACsMwvZ5lFUxGD+DHQ3G6cxNNFGA03CanvQrIfU Gx0MCNVNZ2R11lzzAHXXYMsAz9Zhl320m2an3ULQard9Ctlux41hxXLXPXfSdqPcct5tf83334Cn zXbgTSONN+E7HY54fX5PR/DjkPvseOSUD/ykY+BB3KyGv2K+HZNLStm5/+Jsch665ovTx4N8O2BN Qpbq8eA6M41vDrrtorNFceZU3nvG7MrsfXrv9dosKtzD0xDc8rcNWHwNEuIA/DOkW5qyEtO3XiLy uKPun+nYknZ9EugyJ+9Fwnffg/mhJm+8dTqVN77zt1ctPvjazn/Q4AXi//0OiCkV/NAWJRtEz0y+ o0zGupA9BD4PXxNjoP5Q5D/0PYcLDczB9LJkpCJ1sEc+Qs/l6PYmH8RuPAksQwaDET/5VbB/9QPQ 3Er4wsFMcCD0KuC+isIlLw1tTSXQFPZS+Dqj9DBNYRpTECuwqCOsEBhy+dyR5OSoOnVlUCUIlPTS RcTXUZFOgtIipwhlA//OPLEY1TsfhVTlKRFMqlKcQoqewtUtCjygTlyyYrcUIIGluWCDXLxVGwPw xiJuiYpEeYAd8RioRPLRjyo4I0vMqCpWuQpWXtQSt5AVgVYRoALcCsCp4AhKR0VgATAAZAwapSVL 6gqTtiolJz1ZSlEagJShrMApWyDJlVAyWnGaligDoKxMEsUBV8yTsRxVtGVSAI5/7KKtAHUsahHT WtA6ZjKpyUxixakCz+QlTNKoPV4acWjjqkC5mnmUB0TAWxNQyrq+xUQxivOBgTmnu+DFTqO4E57y 7BY9+0hG1YmFgNJ0VtGsh08itApJvbRF+rRVuYr6K2AWzShVMkZOFb7/LG61m9pHUxe1kaptokIz qeDq0dEwRPR9fUPES5Umt5CmVGRuQykeZgrBnM7waio1m07rwDNdFLVsNrWD5HAGSZISdaFM5Sk+ +PfUi0nVQkgLWcRAmlSn6u2nXi0cWMP6s6GSdT9dPata12qZy7C1rBds6VsZE9e5wgxz3LPrT+Ch 168+ra8jGxtgA5vWwc7ErYbVm1wTC5HFMvaxkI2sZMXgWIRp9LIF0+t1dlEBw3n2s6ANrWhHe4mr do6EJCOtalfLWtKa9qB5TUVnW0vb2tL2teHbnS1ma9ve+vazuK1aC2HB298a97jBlSFqU3vc5v42 ubrbrXOna1voHmi4/zGjrnZZa126BqO42w0vcAFb2E2AV7zo9UV3JWLWnaX3vd1Yr2UwVNmpwfe+ lZBvY7PKXPy+V78Mqe/V/AtfAE82MwT+74GJm+D0GnjB2Gsweh9MkFkIeGYSFi+FVVNXo2Y4vBv+ x2YvbN8PazfE/sAriYVm4hNr9q8ebvF0Uaya2JbivDL2LY1TfMEYe1YAQA6ykIdM5CCLtshITrJn d9yPzfo4x8ZlMj8c1jMoN1fKELaUlZHb1/aa97NJDjORjyzmMi+5y9hdBY633Fos6yOKtVgzm1fr 5iZzds46RnN5MyFnPI+2zlPdM5/9XN0u91i6hL6toT0X50Qr+sU2Jv9Fn8tM6dBS2syGA/Q+VrxT R7dZz2m+sae5a2jdNnrUqtV0ls2E6lSXerkMbvWfQQ1nNct61i/+7q1Fq2p7CHoSfX7vkC8xbEsU O9Ov5vQdgr3rAvTaSmPNbrPHm+tIi4LZu372qss4bWrbtdaxwPattd0OJyO620gjd7kZ3V90q3fb ogbzpZVs6Xkj+czwHoW4Za3ufKfS3ekmr7WvDVp7FznD/V5HtG0tb4MLebQOHzOycw1raf844kaW cMLL4WVN7LvVG+f4wN0L8Hf7m+Qlt0TIw9HxQadc5bQeObBfDnOKg1u2NM8vZJU9h4+jeuXqkHkk fD5qoJ+cQjl39qv/Fy7ppBt9G4Y7Nc2fro2bW/zlVH/HnXOedWvkMNZTX/rWL45xIJO57ALAt2aF 7vKwQ7riDHf7tw8tdawvmudKLXgl0p72AvCd73v3O+ARTl5dc33RcFcF0T3d9WqYu+4pbzw1Qq14 p4v9yZFP9tjtvvZfD93y1a7y4Q2Ldzos3tGSjwZ/2515m2++9UdvO+djP/PR014Sp0906lVveLKX /ey/n/jc2R1u0A8/8Ti3/Vwpn3y5L18YuSf07qnH9ObPfvnMb7ry32r1uF//8mD/Plu/fnXYP7/3 4h8/22vvfO5XP97tHz9944zZ+i8l5uu/fT5Kr//++///asV/ACgM/6s3gAvRcgZoIe+XgCmWfwz4 gBA4BVQVgb5WgBTYZJ53gRy3gBqYJBzYge6AgCBIDhk4giZ4gjhQgijoeB+4gizXgi74DSIYg47n gDR4gzj4NgKYg5owgzwYPPP3g+tggUIog0V4hEiYhDymhCK3g0xoCDb4hFCEfFJID05YhVCIhVAX hVr4Co/XhfRAd2BIfVw4hmY4Wd13hiwkhmp4DIjVhnCof2kYhxLFhnQIRcR3h3qYZSq4h5LwhX5Y h3kYiLFAZYR4iJI1gYjYg0S4iKVwhY74BnMYiZwAiJSYCm94iZq4iZzYiZ74iaAYiqI4iqRYiqZ4 iqiYiqq4iqzYigWu2DQhAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0018_image071.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhmAAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABACQ ABEAgwAAAHh3eGdmZp6dnZOSkoaGhqenp7m5ubGwsNfW193d3cHBwdDQ0MnIyerq6uPj4wT/EMhJ Z0rUKKcG0JxXjWRpAtcJBMujsq6UqiRMl06eM4dQzQCCowBoCYmtm/IELD1yqqfDgllOpFZKzgcY PB4BE2M6GWfPArIVqmKfK+5sPPDljuLxN22gXuZJf2+BN3kIDgc4fYN6IwJPZ4sTkX59a5VpChI2 BTo5nJ0SXg4MYSsHDwIKVaKkK0kLDg9ECaA2EgINOQkEkpUTBRt8ZLYBG4aap8WHWmS4uryGOxJj Q39sWBJ4akIBARwSUgMDmdzemVIGAJyZAG7YABsBnDHtvisPDR/XbAph3tk5eOVC0EtCvHkSDDio YtCHNTVutFnoBNGBHVqg6hXUuBEFu4j2/2CVApktH5xh3wDK+KhmjIgBBDlOwNRRoso7inyRlMmz C8aOFB7d5LghAaKaFSkM+ClhnQygAAwtQMqsV6mqVk9utLlC1SeoQ3cCyLUsbNKuCb6WcYCgwNSh 9+jJ5EornyOgdQHcnUuG6wazJDYQ8Xa2KayNaUwS7dtnXgM7W0J9ucqRMBfLoTq95Sksx9udTwIw MNstx0h7CjEIScnGQZhUvdLlInLTNGHKtEb30sEg5u4pGTNGfaJYBzsJCIgDhAKKT4IwGfn0S6CA iHAKycE8WABdR7tx/XoZ6vC7C4cA1GlnVs+ovfv37ifRKAa/vv37bexZQSACv///8Mlnwg8TcgFo 4IFKXGdFArKMEAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0018_image072.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCgASAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAMAAQAF AA8AgAAAAP///wIRBGKGuZr3GmRRhYvxubbJVQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0010.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
RNA isolation
RNA isolation
reverse transcription biotin labeled oligo dT pr= imer
digest with anchoring enzyme and bind to strepta= vidin beads
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0010_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAAAD/ZjPM/3Bub2b/mYCAgIKBgpKRkp+fn5n/maurq7a2tswAANLS0sDAwMrJydva 2v9QUPj4+OLi4vHx8erq6v///wECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIGJo2LkJGSQAaVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbFq5QLSo0aNIkypdyrSp 06NXnkqdSrVqUpLOtFyIs7VK1zdfPxLbErZN2Shn16QVS5brULcjx2rFoaCuXbsv7urFsdZJXxh6 77oIjPfGX4mNEretQTjwisZ7axxeMvkx5MIoLmOeURkiJp9NOp/QLDgz6bqS39I4vZkEawWpgaJl zNp0bRqij+Qu8Rr26Ne4T5qMSvu0bePBvRZHbqJ3cpCKQTPZ7fr2b+YyqBPRPsL6dc2xoZuSTvkG eMuXDate3pgFaRvc/yUmX56uY8D31SvPkX9w//Cy+eVee/4RVqCBK8QXxP9k6bXQ4IAIqqBggDr8 hd13kKH34AkT/nDYhby9pwKIJHRIoX4YbhgiiSIAh4KJPVjoXXUsBuAih1iVQJ9uKTiXYoY/Aonj fkES2NyNK7IIYz7sEHlkjUjSWOOSO/Tl45MsRlkiVqjsWISVM0p5XpGRvbgemaUdp6KYa45A5T3z eUTciGP22GaSRprpJJq+0Xknm/+V8KY9mIBS6JwQtpaoohqi1sKgfB3oKH5p5lWmhMJlhehqfwLK aAyQosjepX5G+BxbOmKhYI0tiniqFKuyGuYLobrTJBXxadndrC7UCmAMV+LZaYI5propsLoGC+qZ yPKqLK2Z6rQnpa4KOyz/ptNa6qyuj0Y73BS58mojqyz4+mqzdWJ5bQrmpnMruOatm252zMpQrZqB LhsXNseOam+l8NXL3r+f0ruvsQKr0a7BYHl7aMJpLKxvwyeGZtXFGGds1FAad+zxU94SIvEgIwsn QpyBlJxyxTUY5KUbKgMSs3gn1yzzMTNDd+jLbOTch88swwH0HkMHbRbORssglB9F59F00hEjDTUp UAFT9dSbXM3Lxliv8K4eRRUTdtded8OzwkQZkzbZKsDz8M9Ss83C2388Lbcg396ttw157+13C26f /XfXZgs++NRfH644CX0v7rgpNj8uuZyQTz750pYr3njmnHfud+Kes114/+iDG076iZuj9fHqrHON a+uwf5yjYnMJDTEadjMT3WIU4wqXeHTffkbuIhDPg/E4IV+h8GYob0zqUDgfabYKF1u5qr/Da/vB 1/erFvNlSB+MQ7yfMMD56Kev/vrst+/+++oTSz0J8Ndv//3vs8uS6QtmD6v/HJEW+MhgPPEFjHtc MOCvZrM9mnXvdviLoATrJz/frWCCGMwg+vQXFwHOD3cDHIMCd8G/7QAweie8COieMELOhFAMLbTF 6Lw3Ag3aUIIV1J4KbshD+3HQgQlMoYAaCMTa9U6HMJvdAz84vBeGIYa12J0RTyABCQTAile8ohWx uMUtisCLWaxiFsMIxv8ujtGMZsSWBUvVp3Ftpj+XimOlSLWlDgaPic1zIhigSAvMIfFoeAwfl6A3 RBT08JDuy+H/LojIRqbvh6gqIRD4CK1AEnB/5QPkGpN4unIJMTSf7GTxVCBGLmrxjKc0pSrLmMov tpKMsFTjH83nyFpCciWSrFIo63NEsXQDe0RcZC834kdhcnKTmkTVEpFZglraUpbGVJOY2DTNXb1x jgCrox2DGEwGDlOUgtqlEijZK+vlMkbiTAI5u2XHQp0zB39xpiMV6c0UyLORtyTmF9bpSUuKMFrc /CYL07mQYtYzmdHsmTkz2cx7HpKeKGSkQ3mYT4wYNKICLeQxdfbO4xH/lEfdBKc2N5rQ7yEwch+c aA8hOlCJqlSDFVWh2YCZUVCGVKZ3ZKZJdVo97nV0eTfVKEL1iVIafpGVq0RlKV251DAyFalQZalQ aflSmOrJgT+F50eNwE+pUoSQU93pLMUq0nAG1aY1jcgssqpVe1bVhtA8KFXfOsGYImY8yxxrT3ka NbZIMQtdjStGSapCyOW0pHvVa1+VmVfEioCuVr2qYmsI2bpK1qIIoylhBzvUrzKOraLqbEvT6hmw hjWxjgUhlxorV/pV1rKXbS01rTkpN9ZWjti8Zm1NENhVHFa2i51sE3HJULICV7Vl5e1Wv7RczvU2 tpxVqPWK64OiLCAA/xdwAHbTtoAJVFePkiHKdbO73a1016P7oh1gueoA72J3vCJ4wAWui16+eqi9 xYNvAORL37YCj7XR5csFIEABCNCXKAcoXgUO0JUIPKAC6PQnqAZc4ANfIMHYXXCDH6zLdgb0eAS4 AAO6cgEHFw8BE+gKBYiSgPoKFz4hHnHxTIxdFKuYxUDFKnXVsxUSB2DB2N1vV7yLAAq4OLU8DnKQ gbwV+YqAyEb2ry9Ny8sqLSUBG7NAAFb8APkuqYCTvHKWt3yBLq8ttEQFLZqNG2DUnhS8XgDzWRHj QfsO1855fPN651xl0eL0w35G62YLW1SOxe7QGDM0ohc9lYUCuA7P3f+n42ZYt7gdTs0wtHRy6RDp OG/6Dp3uQqg/bVdbjDocay3Eqaf4ObxiWtSa3tvuXg1YrekCKX+j3W9B7bpd2Fpv8BBEr7c2tkvv Gg/FttqZS4dXYa+61ov768rUNmlDkfra2PZpthFX520HSC7eRly4jaapcaOOyubGpHrT/e1ms7vd x373TWgtb33MtN48uSi+983vYui73xx1J8BnQu/wMvrgTlEVwheulEECWroSfqK2WQ1xPAtSxxRn c5uDm+aCw+DZyAN5tkUOZ1jTzOMfb64J+cwQaRtVBA2AeQAaEPOa11zmNJ85zm+u85zHvOc717nQ yym8Ahj96CQ4utH/k650piN9BEpfugiiXgCG+fLRp2WWALYugFILmtVc3zp0yzrCsIRd7EOKuKDP jnazXrvsI2B720f6YmPKPe2kPszPbd5znvN97zP3e+CFLnifbyXmcu+6cosedadLfepNh3rkIT95 qpMg8Yvn6MO5gPnMW7wKiVc83TFbaLVDofNu/zzo2Y53omL9615gfeuRjIW7e971KCf6GLju9emU gfdj/2q8s45c1Wca2yQ3vacxvmfS9rnixPylZuf62vZVkonVpyA7lQlu4ws17L1//vTPHnzGen/t 5J/9cTdp+9RnavrNR/3o14/E9s+f7C7N/vrk76aXB0D/7hN6t0d6/4wDf5xnf/1Xck7Af0o2ZeV2 fqeHgKOkgE0ggQ0YQKWnfE8gewNIf7WXfu5XRBS4gHMXgh74gaLXgcKHbuqkckOQfBi4fM43Ti7o DyzYgixHgzmIEC4nPACYSNtnZz9ofUGoT+vmf38Egia4cXuihPc3ESiTcZvCgUs4WuVjgc8mCbmX ciYXAAx4gXVXT1iYXq/nezs0hOrzhSGXf2gogFV4V0c4gkqghkh4BQyYhZDQbWG4ek44gRpIgn0I hgS4eQJmXeQVNudFA1T4hMRncOJ1iNzlXooIfurnWRm4h/qyAD2mX/xlA8BXiVaIXpr4XkrWiTXw iSoIhXp4gi2gif9a1oA9pmEi4GAQ1j9yKAKu6IexyGCzyGG2GEmEOAMTgGGwWGMpJgIrdgEtNkl1 6ALDmIBBdgE2how4xowosYX9lAPe5WMBMIpNNmQBUGS/CIEosI0T6I1C9mThGGUe0k43yFw6MGIQ UF6PmDZaxmVeZo1/WALySI/zFTb3WGb52I7MZ4BuxorH93Y1OI6BJkowiIkSh3wLqY+DZonBWHwQ uUdkiI1edWfk2IUYGIdewXAk+WuqU5Io6WEiE2utdgh4eItA8W928JL7SCEPWGnUFm2XyAc0+ZFJ w5EZWQo9WQ03yTQsKWuIMJS0JzrDN5NHCWyUUzfDZmpTCZU2A5T/FrNsxKaVsnZvTMOVtwaWVqlq Sqk6AxeRYnOWammDa7k/0teWjgaX1xiVculwdXmXeDkJK5SXBeWVfLmCWPmXyiCTggkQhFmYbLmT iMmDTbmYjvmYyNCYkMkkdDmZB3GYlkkolZmZA/GOnPmZoMkEnhma5ICZpLkOchKYpzkKlLaalOma sBmbsik3qjmbnDCatkkPtZmbt1mGvPkNkvmb2rCbwrkIuFmcx9CDyFma7racqBmczhmdrtl90vkO zVmd7yCS2Lmdskmd3GkNyvmd1PAZ4lme3Qmd5ilD15mezxCe7Jmc1vae8pmZezmfMuSX9ikMxJmf KaGY/KkL7vmfKSSknQJaoAZ6oAiaoAq6oAzaoA76oBAaoRI6oRRaoRZ6oRiaoRpaDSEAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0010_image073.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBwEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAD6 ABEAgwAAAHh3eGdmZp6dnZOSkoaGhqenp7GwsLm5udfW18HBwd3d3dDQ0MnIyePj4+rq6gT/EMhJ q50p3RmU22BodZ+4ZWZqLM8ypHAsz/Sp1YfjIMJDoyDHw1dLCYm/W1FCeBQAnqV0ShUBaQ8FoCCs WobeDThMYyDJ6LT6Oxmcw+N1fC2a0+/4pXPy9trRf3kUgYKFhl8PBhcDQgwBAAEIDgILGiQSAg1D CQR8fQCZm52QUQoPDk+gmg+cngAHQw8MEmZOASywEpeQuJ8ABAktowWxhKYOLxSMso+Xx6kAy44b 0o8AxcRgkQ63Dwi6kpQ31Ry9uuakJb/BC6PpUKdPHqzQvw8BAS0ARwMDC/tjWAQgpi6QQIISjigi 9g/AwVOuABhgRWGBAIfNiByRsCDjBWKK/8y4IwSvwMQxTfDpU7hFnz2VDSukzBfTFAED064N6aTp AMAH/f7NdIkREpGORhOOAQlApNJELWsO+SYhWDEJQy5GTNAwTiCuWJF4JQJWZ8SmQCUM8HmtASKs bkFoYkIxLIgxsjAUIzK26t4LVmNRYFdhDM2wWgEE9hqXT+OIc+0p6TuhAAuqf+zMGWD1rAXOlM22 6WxXAsOqE1gkoFpaNevCYmMH4VuwD2WSpS0QmzVItujcFVmxdt07d2gwifPxvQe7Oa8E2TyXg36c yC3qvmk9OFBAy4RVrOOAv1AdhBsDxNjqtHYWL/O77ytkq3f7zBD2FMZL0C+6vF1FfJQQjP9bApSg 2VHLScdRgsYh2CAFBDWQmGmmeNbdJ8GM8kBjuDWgg3qKbQiKgb4NOCJgIhZIAQMEOBCTaD3ENYeJ KkJYYWU3ipYhXO354ACAprDlRizeHRiWSu8Fcp9y1tSHZJNvBMMbVo9QEhFzVupG0USpKDchBTws UkyRvg05hHcVmJkFJtC1hOYQimiSymZj8lFlV3dGRIwGXPaoFgsLgJiDiDoRdZUbHXH1xFVpthCA ooX6cKijkDKq1h58+INUpNdoih8FBKg2kmDkFYPMBIPKKBijqYLQaliSkjoELC7A+uKrWHma6wKb MhoqK6OCYekhgtxCrHlHDKHOsc4x6+wssxUckAy0ak2bJbW/YautIEIsS60D6iEwLbW4bWuuFwmg Yi4womw77LkSRAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0011.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
divide in half and ligate to linkers (A+B)
digestion with tagging enzyme
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0011_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAAAD/ZjPMMzPM/2b/mYCAgIKBgp+fn5KRkqurq7a2trvg48wAAMDAwNLS0srJydva 2v8zAP9QUOLi4vj4+PHx8erq6v///wECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIGJo2LkJGSQAaVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbFrBQLSo0aNIkypdyrSp 06NXnkqdSrWq0pHOtGCIs3UoV6wifDrp+oYsFbNt0IIUy0TtGrdQ4KaRK5HYFrpn8Lb9utYU2yV6 ywRWMnhMYYSY/hLmW+VwGMdAgRSWICGAhK2VKVsWkZmz5c6bNVfe3ANykcMFUqcmoXr1iNYFyMIu IJmkyagvyOoesVtE7wC/RQgYLgCHaSKFfyvnzdx38xHEh9847q+R9bsnljvfDvy59u7Qoxe3QV1I cu/ouX8PIF56jfL8ritO4va7/fTg87d3TwN+bRP3qYffd/uN1x9YmGBXgv9owGXWGQYObgbhZxJm hhmFl21WIHm4mQAbbx92N5tsrYFYInv7cRjZE/7tUCABMMYIowst+lBjDi/KKGMLN654oBQFMiDk kELOuEKPOyB5w35ENmlkCkr6CEOUNbTX5JUEHNkhkOJdiaUKVLaDynxGhEkDcV56mSWUW06BZppO stlXCWQiVwUBcKa5ZnZtSoFnnl/yudaYWJg5w5+AxiloY3cmGiiACAolhaExIOookXuSQOkMm75g 6aVFLspRgrdNgZeOqKaq6qqZBvApqK1ydxYLrNZqa4wkvHpprPl91E0WeAWYn7DCvQnqkLx2GkOw AzYrIAldHosspHOSUOf/EMJm62yx0Uk7bQnKTrmtts/2yqS3sYb7DTuMlkvusPgFiS617cLr7rjx nittumDRWegItwYsMIzy7kvvrCIMrHCtBR/LL1ZZ9alEw7AebOoTFO9q8Ua//uuEvgaDK/HEVs4r crUejZxEySGfXO/H3XrLwMO+YqMyyTKHunEU6qZgrMma9mutx37mnOzNS+jq6NEQ6/QyFEoDymuv F1MRdZ5MSwlYoy27jPAUV+uJQs/bOO2VCwsvDLAAFY+NdAlpq+0q2xq7Pad112KbG773ysoynFPL WjWPfNtrOLfDJRo41RgZlLd5i777LshYLs44z5EX/i6K0appOdnZKPT4/38uS154jpg+CebIpvdt eI6I4soC6KEnaIrEcecu44auyk7jyLrrznvvlnutNRK0mzA8p28jsbwMyfsYPbTtTde889W/J/To kLspnvVne08c+MfTZwV/Kj4NZA7TR9Y+6V+78f5P89t4PRn1K8Nu/Gndb1jTEVPfW/wnhvwdAx62 E6AaDHi5BaIkgVGxigQnSEGjFKqCGMygU5pmCKiUrxyl6oNZLPjB0FkLgnyACwlL+IxuoHAPeikK C5exvxTmRoYzFEYIRcgpHOawFpLiIXl8+MOEpEwQNyJiERtyxEAoaYVL1MQObRgEKEbxeJ2y4hUJ UUMYEkaJWwyJC504Fv+ihBEQ3JMfz8B4RjtM0YtnYWMb3yAfQjDQN3KcY0v8Yscu5FGPAQFFGh34 BS0C8h93nN0fD6mFN+ohkTc0IyO7EEQqLnCRk3RCJeEoP0lmcgoO6aMcDPnJJgwSDZAsDSZLSQlH 4iGVklklK3NwygH6QZazpMEmH4lEXObSBQEUoQd76clf1qCWqCQlIJRpTBV08ZW+FGYxn9PMU4zx D9FcZjGn2UxkJnMUSanm0DqoCmZ+so5LRIo40VlEWPrKleJk4S7jKU+z0bOIdrnnHL2pz376858A XVdAZ3jNgZZvngb9CUITyhN4MvShEJ3HQquowYpa9KIVBAxGN8rRjqr/MyH14KeWaEE2dxZwFSJd Vi1KutJXOLSKLV0MSVHaxK3NVKaxMCkXJgo/Gm2FAuDCiwIqUAeW8uinQQVTURRwB51ugac/MI0K V+AADDB1DkYlnKj49ACnki8VL+2pT4sCgatigAIKsCpwMBCBEUTAARagQ1YVSVazolWtRGkrHjEA gaYSRKrdwcACuoKBClTgAHHtFQWIggCs2pRwWxEsYQ2LWFltJa1+NaK/HiuuyIIHAxfAoVmImgCg jpKzWvIsYUMryREGALN28GoWXJhSOU2pKVXtK1FMu1gHVFW24sLpkXDL17Wa1ihXLSpKwxrVmJrv pmCtqXBfMVfq0jQs/yxyLvK0Swqo2g+62wVvKZj73ZyitgUDSK9618te9uYBuIsozwTmS9/62re+ sT0vC9rLX/42oAEBAPB/AywCAQu4wAE+MIEHbGACL1jBDQZw98abmLjU4L4YzrByp4ve/no4vXpz HVq+pwP4UqGOtR3pDDLMYvw+2MERRjCDZSzjBmBAwga+cY0dHB726XcFH/5wiA0XnBT5eLx8TPHq aNDiJg9ZOyOOjnF+rIIge/jJ2+KclI/c3du9MLwrbjKLsfwssxhZexzer5X9C+EEw/jNM34xjhOs 4wUDJ8Za3vJXRwGP7DJZzBqeMJSpl70fPRcGa+bvFQokAFWpFMnjbP8CdQAdaDxbGs5t/m+dczzn OG9IR8E99AsS3V4sUC51e4N0WJRsWxlQGsOL3s+nfLdkUevh1IqaHUi/bOsYvNrF5/tb5VQM5j78 DGtaBentJC2IziG71Ucw8Q7C9ihog5XVdmu2w6x9mj9QW03Evi6zBfFtTHHbTh0mtXq7UO5cZxuR rv71fNkts6lVNwXqXm/w9r2qXOVsZrWGt6/lPe8ttNvd1Iy2C/K9buaVWXMlcPa2z80JbAfcBQSf L783viPO/Ttrxa4ywwdg6N60DnHHrtu7u3ydscSb4A4nMsTDE7OuJbxMCx95yZtz8jwnDmhb5TMf XT5wmENv5jJH+c//gS44nKc73xyPOgEytjSKS5G8pXm5vGN+8t9QXXE7Q7eaGW5qXEvt4kLHLtFh kPEJSH3jXz+78Zw+9nyXXeIqD3pD7BnyFrQ91sLOe9O7fWu8V93qmcjnuPdgdrDPnfCFp1u90R7d tfOh5jYfvNj3cHBzU77ifFc4IDrv+cdvnvOT//wmLK76PJAe4Ho//dPVrYXOF0/aVcA6D+Rr9Cu8 3t5UxvfI377xhGU+7KuXbq9f0PYJZMH2iBdr3WnPdRFnOc+KK14Dk6921mve720n/r4Rd/iV033U Oq9+0tdv5sCXXte7Dqbow5xx9bM/PVHGPLhbD9MYjJzk6td1hBYd/zDiJKoDf5WHOX9Wf0dnfX3T foZXJLcXfCjwf+IXPIxGPAf4OwMxaX/XgPf3gANIgKnCgcsHZOkXbCQ2ZR14YeF3gbnzPI92giJH doCnZ3vWDx7IgFUgg6HWd3mwgixIYS1neX5QaIYGhEGIPkModOy0F802PjkIebCAe82QZH5mXmmW ClYIBS3Ha+dnXVtYCl3ohZEGhVpIg6RQhs0AhmG4CvdWTjRVhGhYhRQoCmwoDUO3eHY4hms4h95n emR4h5yQh0jEXW8oh5q1bHUohmoITsuleyWGiFToiJAWiEEjXonIhbSAid/3h34IifHnidsHio+I h5FIimvlUazYiv8apFGuGIuySBVzyIiHYIiH2Dh7iAi4SEa66Ia+2IIoc4sH0YuLUFDk9Fcco3yi JIwRVYn+YIyswIyDII23xDEhxYvFuIyQ8FH8MEwTIYlFlU3kAI4R4V3QxE36QI76gI7Q1IHqyBCh pAjxuI7PWELWiAiKd4+R8YT8CBQV9o8COZAEWZAGeZBBII4IKY/UuJDb45Az4Y4QKY/AOJEWeZGR IJEYCRDIuJHc6GUeyRKqGJLVUJEkeZIoOQfPlJIHQVssGY5q95KjIpMUIX80iRg3iY0jmZPA4I88 SRA++ZMcKUhCCRE7WZS6EJRIGR+7uJTVoZBOKVBR2ZINOZUSFXr/VilRMZmV/3CUXMlnHfOV96CR YlmWZnmWaJmWQ+CVajkJStmW79CUcEkOWDmX2sCWdnmMVZmX1GCTfGlCtviXdCmY4eCXhBkNeHmY GWGSimkMZNmYsSA6kNmXHTmZ+mOZmJmZmhmZm0lDYdmZOrSXoAlEojmanciYppmaArmSqulSldma kQmVsJmRpTmbXbaVtkmauJmbp5mYvPmbUiKbwGkIjzmcxFmbxikJxZmcXISazPmcrCSc0BkSyDmd +pgyvmmdO/Wa2gkJ2dmd4Bmeafmd4tlIzlmeaESe6NlIgbme3qme7hkU8amc5zmfe/CW9ikI+Jmf aESU/PmfJbSPaAA6CPs5oHwQkAaaoAq1mwrqBwXaoHeAoBA6oS8hnRQaSHR4obcglxoaof7ZoSA6 k5sVonXgkiRaoid6oAyaonHwoCzaCxn6ojI6ozRaozZ6oziaozq6ozzaoz76o0AapEI6pETqDyEA ADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0011_image074.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBwEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAD6 ABEAgwAAAHh3eGdmZp6dnZOSkoaGhqenp7GwsLm5udfW18HBwd3d3dDQ0MnIyePj4+rq6gT/EMhJ q50p3RmU22BodZ+4ZWZqLM8ypHAsz/Sp1YfjIMJDoyDHw1dLCYm/W1FCeBQAnqV0ShUBaQ8FoCCs WobeDThMYyDJ6LT6Oxmcw+N1fC2a0+/4pXPy9trRf3kUgYKFhl8PBhcDQgwBAAEIDgILGiQSAg1D CQR8fQCZm52QUQoPDk+gmg+cngAHQw8MEmZOASywEpeQuJ8ABAktowWxhKYOLxSMso+Xx6kAy44b 0o8AxcRgkQ63Dwi6kpQ31Ry9uuakJb/BC6PpUKdPHqzQvw8BAS0ARwMDC/tjWAQgpi6QQIISjigi 9g/AwVOuABhgRWGBAIfNiByRsCDjBWKK/8y4IwSvwMQxTfDpU7hFnz2VDSukzBfTFAED064N6aTp AMAH/f7NdIkREpGORhOOAQlApNJELWsO+SYhWDEJQy5GTNAwTiCuWJF4JQJWZ8SmQCUM8HmtASKs bkFoYkIxLIgxsjAUIzK26t4LVmNRYFdhDM2wWgEE9hqXT+OIc+0p6TuhAAuqf+zMGWD1rAXOlM22 6WxXAsOqE1gkoFpaNevCYmMH4VuwD2WSpS0QmzVItujcFVmxdt07d2gwifPxvQe7Oa8E2TyXg36c yC3qvmk9OFBAy4RVrOOAv1AdhBsDxNjqtHYWL/O77ytkq3f7zBD2FMZL0C+6vF1FfJQQjP9bApSg 2VHLScdRgsYh2CAFBDWQmGmmeNbdJ8GM8kBjuDWgg3qKbQiKgb4NOCJgIhZIAQMEOBCTaD3ENYeJ KkJYYWU3ipYhXO354ACAprDlRizeHRiWSu8Fcp9y1tSHZJNvBMMbVo9QEhFzVupG0USpKDchBTws UkyRvg05hHcVmJkFJtC1hOYQimiSymZj8lFlV3dGRIwGXPaoFgsLgJiDiDoRdZUbHXH1xFVpthCA ooX6cKijkDKq1h58+INUpNdoih8FBKg2kmDkFYPMBIPKKBijqYLQaliSkjoELC7A+uKrWHma6wKb MhoqK6OCYekhgtxCrHlHDKHOsc4x6+wssxUckAy0ak2bJbW/YautIEIsS60D6iEwLbW4bWuuFwmg Yi4womw77LkSRAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0011_image075.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhgwA9AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAACA ADoAgwAAAAD/ZjPM/3Bub4CAgIKBgpKRkp+fn7vg48wAAMDAwNLS0srJyf9QUP///wECAwT/0MlJ q7046827/xggjmRpnmiqrmzrvqcDz3Rt37CM73zvm7qfcEhcBYvIpPCobDppzKd0iopSr1QrdtvU cr9EL3jcE5PPNnFjzW673eix+k1/JwCJ+x3P5+fxMnp6IoJ9hXsme4eEeIhLKnWRbCMElJYilSJB myOcmi+eUQGjATxzknWXAJmrqp4Ar68soSWkpDunqG+qrL2dv5/BsMAttCO2tji5um2LBM56gY2A ftHVfYmEDn+K08i3N8vMa3Ew36MC6erpLuLj5S/nAQj09fTsRkoFBgb7BfC1kNkbeC+fEgUDFAD4 B5CELYIQBagww0OhAgMHGpIQALFjwSpN/xYAYIBR4wiOHjtKjGFi3C6TLlCmjAiyhEs363Lq3Mmz 5zoUPnWWkDmT4MoSUW624cVUWCxiFKESE/GwKE0gLZWSw9S0lVOpX9uBpSrQ6kB8JJJqbdDVV9hh b6PGPXbO7FmsNtcG3ct3J9C+AuTZtXd0qoi1bGGiEDz4Y9qsWhUv/ta4XmFhP/b16weTcuXLmH8g VMjQJKnKllkSsVhSMgCig0HDHSKSZEbXsM3Knn0CsWugn2uiQAy4+N6/fU/GTuGOWY2nc8UGY1J2 5u7QeSNDGQu9GLAjdT2iFd577XPuY2d9d3gOtjrvKYgbn+/3RHF5r3+CgmT+N/tvyvCnnUUImvlj GoABHpTQQp0hg0sTrN3WYBkhjdSafwli6JpcGpLBYYdgfAgiFyKOiEWJJmaRokkorvhEiy52AcKM NNZo440zRgAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0011_image076.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhgwA9AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAACA ADoAggAAAAD/ZjPMMzPM/4CAgLvg48wAAP///wP/eLrc/jDKSau9EOjNu/9gKI5kaZ7fga5s676o Cs90bXvyre/8mPfAoO4nLBpZxKNyCUoyn0wndFqUUq88K3Zb03K/Li9AQC6bz2fwVYxutw0AAxwe j88187u8Xt8bVHl8Hn54dnx0N2xui2QbBI6QGo8aOZUblpQnmABEAZ4BMIqMbpEAk6alm6qXmqyZ G5+fL6KjaKWnuK6cuqsmvQCxsWEktW50dwSBcMmGcAfKnHOAzSB+09Z1wbIttMVlaijangPk5eQk 3d4C4CfiAQXw8fDnIWLsRdry+vP191ux+wIOaOJvy4CACAsMxFHwysGECBdykKIuTUMTDyFG7ECx /2IZcyBDihxJ0hyIkiE7ZNS4T+IrDx6/SbqVipfNl+huwvrEcuNEEDEbzRyKiuivXzlf/sjXs+XP D0HXEc1lVCdSHzrdNdXnsmNMlGDDjvwglpzWrfG6Ag16McRZtAqfwmTb9sNbtGrr4gsGN61cvUEA 9o37F7CQlXg5Gj6CuKlLnGtjLlbZ9/GuEVHLag5LtuyGxhAtXxYRlZtVnUlHd+LJUvToEKWRnFba ivZOwRFdv47s0bRt1bpSb3KHuBxWzEE3KyfZWaw7ACB9EaM7+blv0tQXi5s1ea+wYd33zrAXfgv5 8mvQXzyvHgr79lHg+3sv/wj9+lXwg7uvHwiG/wgABijggAAmAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0012.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
ligation of fragments
amplification with primers A + B
ligate of fragments into concatemers
Experiment
analyse sequence and compare tag frequency
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0012_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAAACZmQDM/zMzmTPMM3Bub4CAgIKBgp+fn5KRkqurq7a2ttva2sDAwMrJydLS0v9Q UP9mmeLi4urq6vj4+PHx8f///wECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIHJo2LkJGSQAeVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbFq5QLSo0aNIkypdyrSp 06dQo0qdSvUpUBvOtFyYuPUqDWJbukYU67WGzyZkGaYt6wKsVq5sZTSaGxZu3E5030pce1cN34N/ +57DVHev4LamzjIJPJDxYY95szj+N/nxXMWL7T72qyKC58+gQ4cmVaC06dOoUbuovNkLY9GwYQ+Y Tbu27du4c+veXVuC79/AgwsfTry4ceCpkydf3ZpzitjQP3sUoRGzCLHYR2S/bmJ7AO/exU03RX2E 8vOmmTc/V8L6kdfRoY+vPgO89vvcS9jP/x1/p/njoYeeeuth0x4W8MUHG/+A5TUYw3798bcWhBCK 56BGAp5HYIEkEDZeFQkqGBpvJJbI2wW0oTibigP8doGLMB4n44zGZajchhyW56F7RYQoonSj2Lhc C6wJRphJVPj4YwSkCakakTk6ktUVRe5T5V3sgKhZlOR1iOCWUWaZJJhhTjkUmTl2IxmaBYo5xZX5 wMmWm1LIeY+dXtEZBZ7z8HmVml8axqUYSv5YipOn4Vimh4GisORoQSKaHpSDknfZms89+hkBnHbq 6aeghirqqJxe0KmppXYqwKoXrCpAq6y6KuustNZq662zSjopC37+ZBCPPXamqWcMdukehf5NmOyy /FlobIC6FqAohwoBO0T/oSIWKxSlEXZboYTMejsDfQ5GKy23gwpkrRDYKqjthy8gC65+4X6L2IXQ 6jptpXo5OmwEpAYs8MAEoFrwqariqvDCDC9s7r78NnrCv0xGqi+6YcJ7ZqaaphItxBFvPFbIrrFJ spaCpmugyBD1ylM3jI6ZMpfwxPymydSuS4TL6vCME5JU4rwe0DKPXGnNOgPhszlL0wRz0j80TY7U MOkJBdXgYP0S0UW3zO+2Xav1tcYoG62ygxKL/bXNLKt9cr9eH71y2XGnC3PaC2m9EtTBZlaH3vFG zPXNftMBOMit0cW30mjZcTjGaSZW2BKP72m53GzTnUTlV1/+NqaUO+65/92LM174HJyrkDpHYBNO eVWwxy77UaNnjHbQM9ueuet1Cz75G0SJMMEfq7M+eO1ukFU877Z3sbwRCzSwFVEUFLWACAsUZfjY t7fNxgUMdDV9+F1VYP7fax/fORzgi//dBdKL4MAF128vd8lwRH8UBQs8UMEDAbAA/dB3Py/hjg8V CJ79SGemsOUhe/VDHfecJ7TE4c9sNJOcVmbHwQ568IMgDOHzKKI4bYzwc0vQSTVOiMIktG4ZLGzh ERoYjRjKsAhusYYNbzhDE/JQhjv8IUaCKEQc1pB2RYwJoKBhlCTGhGzMaKIT1/BCZShwilTcXRSJ iMUuevFA3fviZrJSOv8xgqSMZmSJ+tJYliqyMU9QfOOc4ijHNmqxjnjMIz+spsebPK2PdrwjIP1I x0E6rZCGrBoiE7k1QV5NhJCMpCQnOTtG7oCLusAkQGaBxh5oshaf7EcJO3nJChpJcqTUQShjscp9 5MWRTmjlK2SpD3j8zm2RMyDoeketDqUyB7TMw/BWY70VBBMfKsRbnwIAAcccMx+Y+CUOnpm8C0wg e/AzygMm8IDrQZAoDdAOUQCoOpLBsnFMDF+EpmeA9gWgAgiwQLf6kz1jnkyaN6Bm8tTpvun1R3vK C0A9y2lJYNbwmkSZwFLC94CEXqeYBC1oPmtoBH3aA58GfYb2TFAaHlj/VB4lhJs1LsarsUVGmdD4 GORydk50bmNXGT1bl1BajXOpMn0tDV3ucolRG3x0Ez+96C3zhtOe0iComUBqOow60Z2mKac6xWDz mCoDpUrCquWoFk0bU9Q1RpWXuaSgUyX6IFOSNaJgPSsMsAoJtr4jmlxwqyLkqo1RDlUGFWOaykK6 yxoQqxx0zcYrqVpWjj1qagWc6QFtAKRwBHYbXo0lDIZpgrySYAEVyMZjBavBvvJgfhGkKOYU672H IjSbRdlmNwVKP3COoAEOkOcKTUrYwp7Anf1spzrhKU+yKPQCCZitWo+aAtxyx5/TCx5ZMqsAyjJx grUNHAqsic3fJoWh/w4NgEIdML/NfsG71aCh5kgA3jyUNxo5ROlG+3Fea0QXcZQp6l0Bg9O4mvWU J10sLjOW39LSF3Pvha8/2hvesFDywAhOMIK7GuBBEBiFbjTGg4fLiQm/LcLFsPA969HgQGjYnI6l sBQiK4wPhwzDJRYxFFAcDBP7DhQd9rAUVcySGdPYhWHU6BVvjOP+MtHFayPtc3lM5CIb+chILgOJ k7zJRTJ5ICx+siidLOV/LLnKWM5yGKKs5XvcrcsI0SqYJRLjMe8CqmZOs5p7CMY17/HLbnZljuO8 Rzr7Q7x2FmqeN4nmPUO2s372MqADPY9LERqah67loBOtDr4yuh3JfP/0OiQt6DlTGrKRvvQ30qvp pXZ6HUv89J8tLepSm/rUqO5ymVNNCEezGr2LfjUzMi1rZay61oDgMq5jgeddC4PTvp51sGdN62Gf 2djo7TOySaHrZY9CzM4+M5yjbYtbU/va2M42Dqyt7ZaEutsJoTK4M9HscS/iyuZO9w35qG5J/LHd zEY3vA9R7nkPot727gip8w0JefP7321SNsBDIu6BCwLfBr9FwROea4Ez/OF/cjjE74DwidMB2hbP yLQzfm+Oe/zjweY2yJsh8ZEr0eSJ8DHKQyLylTuh5S53YcljjgZX01zhKr+5HQyt856fcd8+h4PN g14HuBL96GQGOtJPqRjrpQs9506Pei1nLnUtDL3qTIc61ocB4617HdFt/nrNNy52JZdd6Eo/uxeu rvacCLntcI+73OdO97rb/e54z7ve9873vvv974AP/LhDAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0012_image077.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBwEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAD6 ABEAgwAAAHh3eGdmZp6dnZOSkoaGhqenp7GwsLm5udfW18HBwd3d3dDQ0MnIyePj4+rq6gT/EMhJ q50p3RmU22BodZ+4ZWZqLM8ypHAsz/Sp1YfjIMJDoyDHw1dLCYm/W1FCeBQAnqV0ShUBaQ8FoCCs WobeDThMYyDJ6LT6Oxmcw+N1fC2a0+/4pXPy9trRf3kUgYKFhl8PBhcDQgwBAAEIDgILGiQSAg1D CQR8fQCZm52QUQoPDk+gmg+cngAHQw8MEmZOASywEpeQuJ8ABAktowWxhKYOLxSMso+Xx6kAy44b 0o8AxcRgkQ63Dwi6kpQ31Ry9uuakJb/BC6PpUKdPHqzQvw8BAS0ARwMDC/tjWAQgpi6QQIISjigi 9g/AwVOuABhgRWGBAIfNiByRsCDjBWKK/8y4IwSvwMQxTfDpU7hFnz2VDSukzBfTFAED064N6aTp AMAH/f7NdIkREpGORhOOAQlApNJELWsO+SYhWDEJQy5GTNAwTiCuWJF4JQJWZ8SmQCUM8HmtASKs bkFoYkIxLIgxsjAUIzK26t4LVmNRYFdhDM2wWgEE9hqXT+OIc+0p6TuhAAuqf+zMGWD1rAXOlM22 6WxXAsOqE1gkoFpaNevCYmMH4VuwD2WSpS0QmzVItujcFVmxdt07d2gwifPxvQe7Oa8E2TyXg36c yC3qvmk9OFBAy4RVrOOAv1AdhBsDxNjqtHYWL/O77ytkq3f7zBD2FMZL0C+6vF1FfJQQjP9bApSg 2VHLScdRgsYh2CAFBDWQmGmmeNbdJ8GM8kBjuDWgg3qKbQiKgb4NOCJgIhZIAQMEOBCTaD3ENYeJ KkJYYWU3ipYhXO354ACAprDlRizeHRiWSu8Fcp9y1tSHZJNvBMMbVo9QEhFzVupG0USpKDchBTws UkyRvg05hHcVmJkFJtC1hOYQimiSymZj8lFlV3dGRIwGXPaoFgsLgJiDiDoRdZUbHXH1xFVpthCA ooX6cKijkDKq1h58+INUpNdoih8FBKg2kmDkFYPMBIPKKBijqYLQaliSkjoELC7A+uKrWHma6wKb MhoqK6OCYekhgtxCrHlHDKHOsc4x6+wssxUckAy0ak2bJbW/YautIEIsS60D6iEwLbW4bWuuFwmg Yi4womw77LkSRAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0012_image078.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhuwBHAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAC4 AEMAhAAAAADM/zPMMzPM/6urq7a2tswAANva2sDAwMrJyf8AAP9mmf//meLi4urq6vj4+PHx8f// /wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/YCSOZGmeaKqubOu+ cCy7QG3feK7vfO//wKBwSCz6RMakcslsOp81JHRKrVqvNyl2y+16cdqveExWhsvotDobWbvf5DN8 TofK6/g88a7v+3V8f4J+gYOGdYWHim6Ji45lhQOSk5QDBgYAmJmbm5eZbQYRl6I1oZcCqBGoqaut rq+uAbKztAoKALe4urq2uG0KEbbBNcC2C8cRx8jKzM3OzZXRQY0ADDXW1tVAbQDc3t1RYODj3+NH 4eXc5+To4Vns8G3p7t/lQtTZ2Nfb9O3m/vP+8ajX70dAee5sIIwHsCBBgQbV9cinreK6efYaMjSo UeJAh/AUbnx4UOM9jzii/0XD5Ikly1KZWIKKyckArJs4YdHaKatXrls+ieEC+mvoLgXPkip9prLS NJSPoqKhJrXqFapWs9qBqrVrFaxewxYBK7bsNq5m0+5Bq7ZtRLdw18ade5au3YFs78Yla0VKg6h+ CeX9InFwnsJ6+FIhcEBeBAcjCNQgMEIPY8eQRUgGQFnxVkYF6oXm9qB0nwih0Y2uUfoBI8NbUNeL 0LgGggibD6cGJ6I2gNu503iGcpmEAwIJHiQAAAF3n+Ijjidf3jz4VNhrHgx/ox37Ve9pKFtfJP61 3vMKwaP3un291PbuH8GPv2g+/UP27w/Kr/8P//6nqQegI/8NiEeBBtIxwzuCDDbo4IMQRliCXgIm iJ+FalWIoSAabkiIh2V1COJhI4YlYokKopiVCSq+V1mLgL0Io3wnzihcjVKFAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0012_image079.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhuwBHAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAC4 AEMAhAAAAADM/zPM/2b/M6urq7a2tswAANva2sDAwMrJyf9mmf//meLi4urq6vj4+PHx8f///wEC AwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/ICSOZGmeaKqubOu+ cCy7QG3feK7vfO//wKBwSCz6RMakcslsOp81JHRKrVqvNyl2y+16cdqveExWhsvotDoLWbvf5DN8 TofK6/g88a7v+3V8f4J+gYOGdYWHim6Ji45lhQKSk5QCBgYAmJmbm5eZbQYQl6I1oZ4DqDeoqwBt AKusNbCoAbW2t7Oqua2vsLy9AwrCEMLDxcfIyciVzEGNAAs10dHQQK7XUb+uNm3Y2tk/2N7bPePZ 3tzf6t3g5uZDz9TT0tbt5+Dp7+jl9+pH9rz2BQQojmA/geHI8ZBXreG/df4OGgwn8ddDd/gqYoQ4 MKI1hTiYVfKkCRPJUplM/4JKyQlTLFm+XO2CueqWzVqzBuiK1WYmMGVAgyoTWckZyEdI0TxLyvTK 0qZQ7RyNSrXK06pYi1zNyvVj169OtoIdy4as2T1Tz6oFk3atW7FuucKlIoUB0rqE2oohp1cPX799 uRA40A1CgxEEahAYoWdw4cMiEgNYPPdJZcsFxGV25aBzHwiZz22u0dkBo8BbQIuDQLgGAgiS86g+ x9rV69hpLjtxTKIBgQQOEgB4ALsP7xG+gQsnjlsp6jUOdEOXzoS64OJJFzeH9DzuV+ve73YPnxU8 +UXmzx9Kr34Q+/Z/3sP/PH4+U/n28eDPT2c/fzgzBCjggAQWaOCBJXhX3zB/jizIoCIOPmhIhBIK QmGFhGDY1YUaytZheR9SZUKITZFAYomMnXgfhyqu4d8fIQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0069.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Control
Experiment
RNA isolation
RNA isolation
reverse transcription
reverse transcription
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0069_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAADPM/3Bub4CAgIKBgpKRkp+fn5n/maurq7a2ttLS0sDAwMrJydva2v9QUPj4+OLi 4vHx8erq6v///wECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIEJo2LkJGSQASVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbFqhQLSo0aNIkypdyrSp 06NXnkqdSrVqUpLOtFCIs7VK1zdfPxLbErZN2Shn16QVS5brULcjx2rFgaCuXbsv7urFsdZJXxh6 77oIjPfGX4mNEretQTjwisZ7axxeMvkx5MIoLmOeURkiJp9NOp/QLDgz6bqS39I4vZkEawSpgaJl zNp0bRqij+Qu8Rr26Ne4T5qMSvu0bePBvRZHbqJ3cpCKQTPZ7fr2b+YyqBPRPsL6dc2xoZuSTvkG eMuXDate3pgFaRvc/yUmX56uY8D31SvPkX9w//Cy+eVee/4RVqCBK8QXxP9k6bXQ4IAIqqBggDr8 hd13kKH34AkT/nDYhby9pwKIJHRIoX4YbhgiiSIAh4KJPVjoXXUsBuAih1iVQJ9uKTiXYoY/Aonj fkES2NyNK7IIYz7sEHlkjUjSWOOSO/Tl45MsRlkiVqjsWISVM0p5XpGRvbgemaUdp6KYa45A5T3z eUTciGP22GaSRprpJJq+0Xknm/+V8KY9mIBS6JwQtpaoohqi1sKgfB3oKH5p5lWmhMJlhehqfwLK aAyQosjepX5G+BxbOmKhYI0tiniqFKuyGuYLobrTJBXxadndrC7UCmAMV+LZaYI5propsLoGC+qZ yPKqLK2Z6rQnpa4KOyz/ptNa6qyuj0Y73BS58mojqyz4+mqzdWJ5bQrmpnMruOatm252zMpQrZqB LhsXNseOam+l8NXL3r+f0ruvsQKr0a7BYHl7aMJpLKxvwyeGZtXFGGds1FAad+zxU94SIvEgIwsn QpyBlJxyxTUY5KUbKgMSs3gn1yzzMTNDd+jLbOTch88swwH0HkMHbRbORssglB9F59F00hEjDTUp UAFT9dSbXM3Lxliv8K4eRRUTdtded8OzwkQZkzbZKsDz8M9Ss83C2388Lbcg396ttw157+13C26f /XfXZgs++NRfH644CX0v7rgpNj8uuZyQTz750pYr3njmnHfud+Kes114/+iDG076iZuj9fHqrHON a+uwf5yjYnMJDTEadjMT3WIU4wqXeHTffkbuIhDPg/E4IV+h8GYob0zqUDgfabYKF1u5qr/Da/vB 1/erFvNlSB+MQ7yfIMD56Kev/vrst+/+++oTSz0J8Ndv//3vs8uS6QtmD6v/HJEW+MhgPPEFjHtc MOCvZrM9mnXvdviLoATrJz/frWCCGMwg+vQXFwHOD3cDHIMCd8G/7QAweie8COieMELOhFAMLbTF 6Lw3Ag3aUIIV1J4KbshD+3HQgQlMoYAaCMTa9U6HMJvdAz84vBeGIYa12J0RT/CABwTAile8ohWx uMUtisCLWaxiFsMIxv8ujtGMZsSWBUvVp3Ftpj+XimOlSLWlDgaPic1zIhigSAvMIfFoeAwfl6A3 RBT08JDuy+H/LojIRqbvh6gqIRD4CK1AEnB/5QPkGpN4unIJMTSf7GTxVCBGLmrxjKc0pSrLmMov tpKMsFTjH83nyFpCciWSrFIo63NEsXQDe0RcZC834kdhcnKTmkTVEpFZglraUpbGVJOY2DTNXb1x jgCrox2DGEwGDlOUgtqlEijZK+vlMkbiTAI5u2XHQp0zB39xpiMV6c0UyLORtyTmF9bpSUuKMFrc /CYL07mQYtYzmdHsmTkz2cx7HpKeKGSkQ3mYT4wYNKICLeQxdfbO4xH/lEfdBKc2N5rQ7yEwch+c aA8hOlCJqlSDFVWh2YCZUVCGVKZ3ZKZJdVo97nV0eTfVKEL1iVIafpGVq0RlKV251DAyFalQZalQ aflSmOrJgT+F50eNwE+pUoSQU93pLMUq0nAG1aY1jcgssqpVe1bVhtA8KFXfOsGYImY8yxxrT3ka NbZIMQtdjStGSapCyOW0pHvVa1+VmVfEioCuVr2qYmsI2bpK1qIIoylhBzvUrzKOraLqbEvT6hmw hjWxjgUhlxorV/pV1rKXbS01rTkpN9ZWjti8Zm1NENhVHFa2i51sE3HJULICV7Vl5e1Wv7RczvU2 tpxVqPWK64OiJCAA/xRYAHbTlgAIVFePkiHKdbO73a1016P7oh1guboA72J3vCJgAAWui16+eqi9 xYNvAORL37YCj7XR5QsFGhCBBtCXKAUongQK0BUHMEAC6PQnqAZc4ANTIMHYXXCDH6zLdgb0eAOg gAK6QgEHF88AEOhKBIhygPoKFz4hHnHxTIxdFKuYxUDFKnXVsxUSB2DB2N1vV7xrgAi4OLU8DnKQ gbwV+YqAyEb2ry9Ny8sqLeUAG5tAAFbMAPkuqYCTvHKWt0yBLq8ttEQFLZqNG2DUnhS8XgDzWRHj QfsO1855fPN651xl0eL0w35G62YLW1SOxe7QGDM0ohc9lYUCuA7P3f+n42ZYt7gdTs0wtHRy6RDp OG/6Dp3uQqg/bVdbjDocay3Eqaf4ObxiWtSa3tvuXg1YrekCKX+j3W9B7bpd2Fpv8BBEr7c2tkvv Gg/FttqZS4dXYa+61ov768rUNmlDkfra2PZpthFX520HSC7eRly4jaapcaOOyubGpHrT/e1ms7vd x373TWgtb33MtN48uSi+983vYui73xx1J8BnQu+BwyneBifqoxOO2UIzvFgIf7jEJ54IcFM8vQu/ eGkzrvGOe/zjIA85QEXecI6TnB//Pnk/Uq5ylEe85TCPORdYLnNC/bLmBFFIwXF+jJ3zXBgv/7nQ h36NzBIdH5Q+OtL/Ha70ffi86a8oN9ThNHX5BL3q2pA21uWh9a23A2VeJ1TYl77usbvL3WZ3F7rT /oyns50TNH/7MqQu929YvO7lcDveWXHzvWedcn4PvOAHT3iV673wGUE74tuu+MXPfe2Oj8XhI98R plN+fN2+PC/urnllTL7zKck86KM4+rZfvfSjiDvqGyKnz6/+DUl/veRlT/va274Mrr99S/qu+1So vvesADzwE3L64Ru/2kY/viZir/xMiL75kfg99PEm/Ok7v/rWnwTks899bue++12QPvj3IP7xlwT7 5kfE9tPP/nwXv/13KD/856Dz+RuC+fYPSf73z//Cf7//ULB+ABgTjf83gAFocgZIfwWYgEywgAx4 BAL4gGjQdRIYf41XgXYAdhi4gYTmgBxICRf4gXLwGSJYggzBeSYIeyGYggSIgCz4guQQgTCoBRQ4 gy2xgjaIDNaWgzzIdfzSg8Nwb0Cog0MYE5ZXhGFQg0iYhGW3hE74hFAYhVI4hVRYhVZ4hViYhVq4 hVzYhV74hV4XAgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0069_image080.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhfgEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABABs AREAgwAAAHh3eGdmZp6dnYaGhpOSkqenp7m5ubGwsMHBwdfW193d3dDQ0MnIyePj4+rq6gT/EMhJ q7046813SM6kKJSxPMswdtIXYiY6sHRtT+5N57oH9sAMz7IKWh7I5INxEEgISEeAonxYCo8CDas1 WhxIEUmCJQBAxQ7YeimfX944ay2/0OuTO76nr6TrAUhOAAMOUhMJDw6DLQ9TFg0PCTSRk3sAYRcM bD2ZFpuXewKcR6RxoxOee6qhNaytVKQBhoxIY6kXAguGLLq8l68SwTTDw7A3A6axe8mpynLGxxjR oawIDwepkQxUFwgGm2Yc3+HAz5jnLMXp0r1gGdQ2Au/C7EHx7c6wBScKj+gVRi3ApUASLgsDr2Xj kBAbhRyJHGhJtEBcgANSTiys1+IHFCUA/4YQAsPgH4ETyZR9THJx0YIxAiI9UNBFQsQZIX9EnIhC nIRCS6ZAVGSmYJILnoCW7Bgios8zinAaDROG4j8cGANorFCg4IKa6K4h4QZg0wMCQyVCrTihK4ou LXWRaKnVIUerTFucuJZ351ozC6YcGMixgicr8x7gVFZgEhTCGhoDeExhjQEAWBZcpowuC4BICPQB 0ONJD5YAgQjPagDAwDoJawYMIHwiABQ4iQi4joLkcubNKNo6Sj269+TgAKexOU28+APgkHPvtnL4 OWbksTyDngDl8qaaYVzfArDAieXrmo8T7l7Wc+zZnbVsR+cb+Z3AIdmc/61+tAQEcLxyWP8jnimT wCNIXHXBgfVcVZ1oyTWX3ISqeDJVJokgeE51jACgAGEPYkJWiBNeyEaIxmRiIoQojsgJicvkB1kk ZMwk2iY4DRBaYRSyQeN1YwgSY3MkZvIAa4a9qCQmSJki0EGEKBJIKUpspFyVMfbI4pI8VshlZV8m CSEFKpR24gtFhtnliekY+QyJ9Ciy5phb1lkYZ+OlOeeEe+pJ3kxW6ulIKRVcYwmTE1xDzwQGXCaB a5Bd0Cij2Gm5p6WY9qggjFmyopUCH02QjAFQ7OgnOptymSKbCmI6aqmXBnMqiZsgQMChmLopGiso fumJTBsJyto8Y84CB6KIsFLeBKMMisH/shI0+8+sbIyCZK6kWHgkAMT+aUYgG6p5Ao+fGbIjtiJs 222v8LBR0LABhtmAuXbyKZq16XaxLY+3NdChnwXpiySv1e576mSJ1EtVFJY0O0ghhzSiYEEUJDCe h/taYLEfGVN7WSQ+hQhuLZykhIQl4l1XKYGDEIxaghI0QeilJhtkKRgBkCVmlEkcSqLMFeDMDaf1 fHwWdza6FjIpBekcKxtQkKA0ufR9djS6mEyhi8JWAGhzFWOd2xkrcJil5FEVmO3MUSC1jTYS16Sw NnVsv402AF5fi7chszCY5MKVJhPYh+JU4UDNYyf+HxgD1x1GMv5QyUbecytu+AyQgwu4iTJwy8BV P+DdncxTbm9OBuj1VNq53ImXTt1s+LXuuD03dJhPu7dLA1QSx+buxe5RdEI7B1q1g4/vyONxfPI2 yMZspMz34Hy00BMz/AYI4HTM8tF3fwP33m/gwLkHaB++DeNPUL7wQYDReyvgny9/k/HPT4FbM4Fl fwf40yR8/RJQgAOeAr+73S4CADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0069_image081.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVgBuAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABT AGoAhAAAADPM/4KBgpKRkp+fn6urq7a2tswAANLS0sDAwNva2srJyfj4+Orq6vHx8eLi4v///wEC AwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/IASMZGmeaKqubEtC oivPdG3Cdq7vb8z/wBUuSCyOhsYkD5k6jJwHKCA6fU6lVaozi+1eq2AZU2n0zcZkolm8TgfbLbRp C5VqrXcuuL7va0VbbG6DgoSGKnKHh4mKhIyNbo+QZJKTSZIBmZqbnJ2en6ChZ3CWLqRCp6VCNpWq J6kpmKGztLWgha6DrblLsLw/u785wcI1xMWjyEqyts3Ot6a+xdI31L/WPSzP29yZuMpq2OBx4uOo 5uHob+Xqr+ztJczd87Tf7eXHrvjvlvva9ABFRYPXi+COfAaP8COIMGFDg/ICStRkT50/iKwWQrqo YqJHigMTZhQ5kiSNhww1wd5TaRHWx48V0XGEN/NeyY4vJ8Y0iYilTJ/mUNIEOk7oyn85A+4sepNm U5vGXCZVGpJn1TIwYBSAgABAVgAFGDgiGm/EVq8FvI5IACFtJKBbGxx54dWBgBgKEjjQBZSBALpz IQxgEOMBDAJvi4iNEeOsCLYjxA54kJiIAQgLvGZt+1Wu4QRsyWazepV0T9PkULPYzLq169ewY8ue PXuSaGW3keWepnp171W/YwUXPvxV8Xibj2v+qpx5c6MWlZeFFAIAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0069_image082.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVgBuAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABT AGkAhAAAADPM/2b/mYKBgpKRkp+fn6urq7a2ttLS0sDAwNva2srJyfj4+Orq6vHx8eLi4v///wEC AwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwX/IASMZGmeaKqubEtC oivPdG3Cdq7vb8z/wBUuSCyOhsYkD5kSOJ+lpzMqpUJJ0ukoK7AxlUbf7AsmimXkMvDsSluv2ypW HocDuHP6mK3up9x+gUd8goKAhX2HiGWKi0qNjmGEJAGVlpeYmZqbnJ0BaJORLKF/pKJ/XqanJ6o3 pJ6wsbKZoKuBkLZLrbk6uLw5vr81wcJ7xY+ms8rLmrXHKrs9z9CpLczXzM7TQcTb0NHe3+Fr4OOs 5eYl3enSK9jvs9rm5eui9Oj21e7w/J3y7DTqsROYjuA8fAMRFnzVryGtNgoX3QM4caA+FQ4zWvoH kGPHFgbHhQw30lvJbYo0u2r0iPLiPJciYZ5QmZHlR3E3jeW0udNVT4g/QTKk2Y/nsYoFZZpUWoJo Q6NBT6qDAcMABAQAqAIwwMCPVHUjrGY1kHVEAghk1Zy02uDIi6wOBsRQkMBBooglGAx46xYCAQYx HsAooBYvia4xYogVcXZEVwIPChc5AGFBVqpotbYVnOCsYRRfj35GOXpa6GKYU6tezbq169ewYTsq bTooSNujcAvRTY03aN+/gd8Q3kMrcczEL9MWndztohAAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0070.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Digest (TaqI/MseI) and ligate with PCR linker
PCR amplify and separate on PAAgel
C
E
<= /td> =
Isolate and characterize differentially
expressed DNA fragments
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0070_background.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh3AFlAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADb AWQBhAAAAAAAAAAAmTPM/2b/mYCAgIKBgpKRkp+fn5nMAKurq7a2tswAANLS0sDAwNva2srJyf8A AP8zAPj4+Orq6vHx8eLi4v///wECAwECAwECAwECAwECAwECAwECAwECAwX/YCCOZGmeaKqubOu+ cCzPdG3feK7vfO//wKBwSCwaj8ikcslsOp/QqHRKrVqv2Kx2y+16v+CweEwum8/otHrNbrvf8Lh8 Tq/b7/i8fs/v+/+AgYKDhIWGh4iJikIFJo2LkJGSQAWVlpWTmZqbMY+PAZ+coqOkoCOhpamqip+o q6+wgZaxtLV6rq62urtql5e8wMFnnsLFxma5x8rLUb7Mz9BPydHU1YyO1tnaOr6z29/gMLjh5OUo 4+bp5ujq7eCh0+7y0L/z9vf4+fr7/P3+/2S8ARwIqxXBg6oMIlwoylM8hhAjSpxIRCDFi4UUYtwo 6xTHj380ghyZxyLJk3ge/6JcmaYey5dtVMKcOUYkzZs1XeLcCUYmz59WbAIdGlQn0aNTiCFdesUn 06dBlGq5QLWq1atYs2rdyrWr169gw8oIS7as2bNoxYZrxHbLBVJvY8QdNfcbJqdM6m7S24KvJr9I AUsSnIJwJMP0MLmFO7YUYmZt8S55nIgyCcuIMBuLvJhuY8bvQEmeLCOC6dOoU6uOMIJBAAauXb+e PRs27dixReAWkaC379/Agyf4DENzidXIV5MgwLz58uYEnjvnLbw68BvGZxpPzv20EevgfRN/kX1E 9+4/woPHDnXE9vPJv6u3Pt5FeRHw4/uYX519+wDv5aeafPwFV19fNgio3P9+BV5nw328oDLaEQEq 6J1ur+VWG4a2ybabhxlS1+BvB7IAoYWpSTedCNBFN0KLI4xI4oNrYXNFhSgSKONwcpX4Aoqopbcj jzVAuItiHt0IWnGO1YjkhEUY+YdxUk65lkNQRrkkeU1eaRQVVfZBZZd22WhFmHyMuaU27FRxIpCs CTFkbz6q8CacQu7oX5klZDnEnTkOIOighBZq6KGFBjAnkVz2WAOcpgWA6KSUCqrokHva5QwWgFo4 xKJ1FpYgpHnKmOk23WTRqYKfzhkqCquyyqCeNIbWJ6ejAtkqpo4y+Sips5paK58k+ClErPlJWumy k17Kq6/QzgApa8xWa6n/q8OiaqabueYoJ7bR2tettz2ASwOatKSKq2e9ioLuR+/moSa775h05poI 4msNMUjeS2+j/7LpkbHHjiurDuZmK+6veCKccJE1iiAVtwwHai2lzgqLw7zSknpxs6DmEO8rAhEM BLLw8fDwua+uMO0OIW/8H4AGC6jys6fmS8PLDuOsMFMon3czrTK3WxqwOcSc875Jqlpzsh+D7DPE RsfwctSHZqzx0oE9nTLMU7NcNQw8J73yDCNjlLYdHHOyNkVv09H2XhEXu667LS8S9yjd9Aumvivs PYfgnMDj9xSEwzH3X+uYXHDFnuo4IsCUH90wEUoH7mUXQaOHddRaN1i5/84d4/n5xaGLvvDmU3kt tA9+nX3C4j8ivSvRJtYtmuOwu45e7ybIbgLtLpR9+9aa16uuvztfzkPsYcOadwrGfxv97LpLfHfp Fp9eber8jZ475J56/33mdpJjL/NuT19Z3byfDHj680cjFMV4j51J4prc/3fApGsf61oHwPEVsGs1 EF65rjc8950AfUCAoPRsZTclJRBT5jsfA0tAvBaAKoPLAl/4Voeqvm1PBgoUWwlS6B4HBo+FZtvg ZbJHQBrAcGw37CALJBgs5NFPeVyA0A3DJSIfikp/LuDhAmXYwtC0pYYzcBUIQ5hDF65QilOUGhNF wL9McAaKdEPiYJwUP//5HfCH+WtPF9ugQ0iscRLrw58Aicg4CpYReGkU3/7g15nm6eoHEgikIAdJ yEL+zIDcIxcPCsnIRnKNGg7pYyJZlcVBBaCRmBzkIZPnR11VcgCXzGQmH5mNO/agc9wBgihHSTU6 1s55O1glJklpvzgiznepBKQsGblJNFrujz3YJS97ab8voFI/PhCmIVupx+LZLpbK1CQx6dG0E1rN dJ8MZTQDOU3skY+SldTmNmn5jE057YxHRCc1vfDGNbRRbzQ0pcjqN0F1ltOEFswjCeeoLXbS05v2 LKc/v5msUk2Omfu8Jix7ZkRXQtIU8tzBMZGjrHAOsYFiZMHVLLrFdGr/64nn7GTknidC9XQToyId 6dAa2sxaHo59v1SpDt5yUQ5akQTVY+hBEVrKauZzkjY7ZUnDc1KbErSgS2RpQvf1JTnGFJwcxZ0V J0rRbNbUqHyKqERxicyk7hRtNzXPM3WqOp4Sy5qTsYoCLtAAAFElAAqYAEwD2ISrrLWtVYGrXJ0K NCnEZa0AUgDNAuCACwj2fw5Vwl/fYtjBFvawt/xPO+F6AQpwsYUXqIAB4vIAB1SAr4hUwlotO1ia ZXazIujsZxGbDq0W9QcTMEATTXuACcTFAlRBAGuXeoTYzpaxtb1tbnfLpuWB1ghyjctiaVZYEcj1 ABYgbmiTkNzLUpa5/3F5bnQjSyzXFm0JC7gABNxalbW+1bK4dUBhJ4vSlhIhvOO1a17RewH1vtWv fARjHRN7GPW99LhjzKgbZ8ZeNLxTEQVOhP+4y0/eTiLBiNiUd8+Vlgpb+MIYtspYMszhDnuYKzTU ZzAgjA9bBtgYJLbHgvt7jBTPY8UDbvFTYAxPGc/4vw9WhotnNoYd18HH7WhTjm28lL5N2A1AHtyN cezGq4xYw0ihcWXy+uT7Rtmn+6NylZki5SmjOMnqMPFhwKw4Hpv5zJS4C5rXzAPOHJnNR3EznOdc gycymc54PkcF88znFUioz4BOgeECTWhHiLnQfVYzohd9ijczmiaOfv+0pCdN6UpvJNKWHskXM03n TXMazk399Jm7LIwPm9rCkjZnNS5g5Fa7+tWwjrWsfUHmiExs1bPOta53netaUwTTCOa1sIdNbF8z RNXUYDWxl81sWBt7IcA+TLOnTe1LPBvad9Zxtbfd7GsfhNQj5ra4h+1tgigk2oVQ9rjXPetyD8TI uGa3vJ2d6nCoe9747oa7Rf2Ge+f73wXYN7/ZCPCCB3zg0fC3weUt8H7MAt2ZWfi/G74POWdD4RIf N8X1YXFrYDzj3N54PuwMcUN8HOTVFvnI9xxvlK9b5RzPdotdzm6Ylxii2jg5zZlt83t4Otk713i9 ZY7ioIu75wgXg87/jS5spL8Yy9BYugCmTvWqW/3qVYc11rfOdQG02unyQHbCmb5tsLvj1kAnO7XN ruKcq33tqQ41M6Te9bpTXet2t/vX4070Yiz97e0euikuDvhuC77k6S48z4eO+MQrvtiWbvwg/v54 V7M9zMaNeuXJXW/tEX7zvL580t3y6rzrvfSm7/reHw1uYFAe9Prmu2IkPyXY71r067C37XWN+8bR XhCv330leq8+qD8j+MInfn49LvzAs15ivwcE8nev/NG7yeVXv4TVtZ/11TMa7WNvfqyrXyaQtlz8 lp+0opmP/leT3/p+bb/74a9t+Xuf/qW2//0XfeiZ679v7ycwnsd+/62WeqqHegaIdftXaK3HC9Nn ewHYUwN4fv9nCRFYSn2XfwiYgHcHcheIf5OBdxzYgRs4glO3gITWgLvwdyZIghL3gZCUecdXgbH3 fJ4XfWlCg7T2aTi4Bw8IezBYQhRIg0EIghSig9bWeSw3g0g4fLInd0XXhAdngz3oBz8IekVYDVWY g1KYhZABhfXXhF64DFtYe12ofhkYbmf4fOaXdmJ4eG63ht8nGttAdy3odbF2h3hoZGNIhuBwhZvX h8fwc+H3hmw4eASIhIK4Gf0Xha7mdScIiZBYCZJYAHi4hxm3iMVQhlYohVM4hxPohor4hHFoiKDI iXoAiJWnicqAiv94oIqPx4rBgE+iqIOyCAwqqAuwqHi3aIQS5Ym96IsiA4zC6HrEqISlOIqH6Ip3 sIuFF4y6QIiaJ4f814h+d4yg+A0seIeypofpZ4OIOIQVCI22IHbTaIr8F4q1SISRl4y2SIqfh46I xoypiI3fl4bGSI3zaHxzZ4/7eIPi+H/kWAvwto7jiIzuyI7FuIL+uJCxcGoQeRY86JBDQYsUiRO5 eJGXxo8aCRPW2JEgGZJHsi0iuRIFWZIsAYYoCRIZuZLQxpEuyREtGZPfho80eZM4mRE2mZPmBpM8 aWs++ZPHFpRCiW30WJRImZRD8JFK6Q8z2ZRPB5BQCREnOZUIcZSoVpmVWrmVO4GVXLkJTPmVZ+eV YgmW4ViW70aWaCkJarmWCraTbukkZxmXK9eGdHlzcHmXermX4qCOfBl2OPeXJWaXglmYhvkC4HeY 5SCNiulfc9mYkBmZuyOZrRWYlCmXbXmZdMAWmamZnql+JPmZiSGDogkZpRkxnXmaMWGZqhmDj9ma sBmbsjmbtFmbtnmbuJmburmbvNmbvvmbwBmcwjmcxFmctBkCADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0070_image083.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhfgEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABABs AREAgwAAAHh3eGdmZp6dnYaGhpOSkqenp7m5ubGwsMHBwdfW193d3dDQ0MnIyePj4+rq6gT/EMhJ q7046813SM6kKJSxPMswdtIXYiY6sHRtT+5N57oH9sAMz7IKWh7I5INxEEgISEeAonxYCo8CDas1 WhxIEUmCJQBAxQ7YeimfX944ay2/0OuTO76nr6TrAUhOAAMOUhMJDw6DLQ9TFg0PCTSRk3sAYRcM bD2ZFpuXewKcR6RxoxOee6qhNaytVKQBhoxIY6kXAguGLLq8l68SwTTDw7A3A6axe8mpynLGxxjR oawIDwepkQxUFwgGm2Yc3+HAz5jnLMXp0r1gGdQ2Au/C7EHx7c6wBScKj+gVRi3ApUASLgsDr2Xj kBAbhRyJHGhJtEBcgANSTiys1+IHFCUA/4YQAsPgH4ETyZR9THJx0YIxAiI9UNBFQsQZIX9EnIhC nIRCS6ZAVGSmYJILnoCW7Bgios8zinAaDROG4j8cGANorFCg4IKa6K4h4QZg0wMCQyVCrTihK4ou LXWRaKnVIUerTFucuJZ351ozC6YcGMixgicr8x7gVFZgEhTCGhoDeExhjQEAWBZcpowuC4BICPQB 0ONJD5YAgQjPagDAwDoJawYMIHwiABQ4iQi4joLkcubNKNo6Sj269+TgAKexOU28+APgkHPvtnL4 OWbksTyDngDl8qaaYVzfArDAieXrmo8T7l7Wc+zZnbVsR+cb+Z3AIdmc/61+tAQEcLxyWP8jnimT wCNIXHXBgfVcVZ1oyTWX3ISqeDJVJokgeE51jACgAGEPYkJWiBNeyEaIxmRiIoQojsgJicvkB1kk ZMwk2iY4DRBaYRSyQeN1YwgSY3MkZvIAa4a9qCQmSJki0EGEKBJIKUpspFyVMfbI4pI8VshlZV8m CSEFKpR24gtFhtnliekY+QyJ9Ciy5phb1lkYZ+OlOeeEe+pJ3kxW6ulIKRVcYwmTE1xDzwQGXCaB a5Bd0Cij2Gm5p6WY9qggjFmyopUCH02QjAFQ7OgnOptymSKbCmI6aqmXBnMqiZsgQMChmLopGiso fumJTBsJyto8Y84CB6KIsFLeBKMMisH/shI0+8+sbIyCZK6kWHgkAMT+aUYgG6p5Ao+fGbIjtiJs 222v8LBR0LABhtmAuXbyKZq16XaxLY+3NdChnwXpiySv1e576mSJ1EtVFJY0O0ghhzSiYEEUJDCe h/taYLEfGVN7WSQ+hQhuLZykhIQl4l1XKYGDEIxaghI0QeilJhtkKRgBkCVmlEkcSqLMFeDMDaf1 fHwWdza6FjIpBekcKxtQkKA0ufR9djS6mEyhi8JWAGhzFWOd2xkrcJil5FEVmO3MUSC1jTYS16Sw NnVsv402AF5fi7chszCY5MKVJhPYh+JU4UDNYyf+HxgD1x1GMv5QyUbecytu+AyQgwu4iTJwy8BV P+DdncxTbm9OBuj1VNq53ImXTt1s+LXuuD03dJhPu7dLA1QSx+buxe5RdEI7B1q1g4/vyONxfPI2 yMZspMz34Hy00BMz/AYI4HTM8tF3fwP33m/gwLkHaB++DeNPUL7wQYDReyvgny9/k/HPT4FbM4Fl fwf40yR8/RJQgAOeAr+73S4CADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0070_image084.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCgASAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAQAG AA4AgAAAAP///wIRhGOJCavcoDuytoDx1dvxsxQAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0020.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> <= /td> <= /td> <= /td> <= /td>
RNA level:
• Northern Blotting
• Dot blotting
• Real time quantitative RT-PCR
• RNA in situ hybridisation
• RNAse protection assay
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0020_image085.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh+QAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABADs ABEAgwAAAHBub4KBgp+fn5KRkra2tqurq9LS0tva2srJycDAwPj4+Orq6vHx8eLi4v///wT/EMhJ axVnTcYs1la4dWJplkXzNMYJfG5cwnJt3wCHS8/TSo7ew7TojSzFoU23sw0eBEDGlWzKqtZsk4l7 FCqOg8t4IpcCSu0teCOj1SEzfE6vLEgTREwu4lsMaXVlgXtKgII8hIiLTQcPARR6Y4oWfhQBRYwm lptDmJRqnJqjJwIPCRMGPxIBCD0MAxNkNAAEKoBpra+xDEJDtAO9DbESMI4LUSHBK8QEvoq6D7Cy Q71CEgZFDgLFCQsBDSTZD9sTtj6c4+UAvs5GAt4CKqgv3uDi2tzFKgpK8g/9ik2RsGzYBGMPkElg AEJKBRUCnDUkg0XAAj0FzECUmGgCFmdf/4IQS/LFWYMQIAGIpCZiY0KW7NI8EWDqJIAkqk7OrFns IoCMlHaumOBoQIF1QmK5UnCzR04AQm0CaMDNlASqL5R89KLywcgeJYcCAPRDDAUGNs2oVeJInxm0 HWNSIOOKoDSYci3UhXo3rgW4efO+fUYN0kLCUh64pWTt2gaxMHnGNJwDMTtJsjDD3PsEz1qPHQYk q2DAGl6KafiUJiOHderXeOfC9hti9ezPlQL5yUQ7NgVnDirgbk1JBQN6U6UhFzw7sJkEipleCOfu tBLcL6i7lu0XuyXvoOQxqB53rT7u1M53p9RDvbkeo51f1604hKtTeXog374eL5oD0lGgQv9/cakQ hSlpDMgcer0Q8wBm2J3lFQ8QgqLgcGn0ogcmsWkIAIfJHWiJhyACMcACUuWFhiRykNiQBAQ48puM cTVIoXUUOPBNbjS1R2BGofWQlmIIclOEAMHl5UwHGY0W4XtMQlFMD5TJ1mN9eR0ZHC49mBXYWL6Y BSRfkKUSJivj1fKAl2AB4EoycnC5pizcgMNDnVKRseRPUhKIDR4UAEIVWlH4EhMZCiywgEUH6COo AISCyQA3hkJlHDGHDlFpBQNcSo0lj0ZqKCCTSpDog5+meKpmiS66QKMhrPpppon0w0IiZcoqiwEN YMUOr75W2qk0mBq6KSnIJqvsHKIs6+wZs9BG20Sz0lZr7bXQUovtttx2G4pj3lIQAQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0068.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0068_image086.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh8wAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABADl ABEAgwAAAHh3eGdmZpOSkp6dnYaGhrm5ubGwsKenp8HBwdfW19DQ0MnIyd3d3ePj4+rq6gT/EMhJ KwjJ2c27/2AoUpg2VopyWuXqvuLwDJ7zPHCu75yNV7fgbWEQoFQ5H2/5YjwSnxtzIvhNrxsp62YE EByOAFY7rWI3ggY4amUS2uczGdgOgLvM+fIdrxwQCw8FHno7AjZ9fYUSegcPBleLOYdwcQ0AjpAV BQ0Pbw+OQxKBggJODwo0FxkACQ8OgwpCAKY3qRSuDgQVXw8LYhedjminty25gxOcnpJ6VZerJgHC P7WoqhLLn6G+ozcF1rfRra+xs+HY5Lq8Nr8S06A4A1AFD9ATdgwACFIBCvcAFBjpFKCeCR8IANSD RoagwQmuCvDTIiNAAHsSGoi5uMHhKwAI/xVifOdA30QOhbQoAaDxwg+PJi6U3NfvHwWBLB8U/Ajy RsKFjKzAhCho4o+KF6G15JgA2A1gElw5PfokW8KADK2Q0ULGJoCtP7pJkDXrq74OXrlqtSI1KMpK YBmdnZCWrU63AGRAUXi17oS4X634xSs2oJCwcwML0SQBEd6cEhJ7Ift37WMJBCg3DhuzUCcFjDdk Vlt5guPAbys8K50TNC/NPVlDBiB5tGXCcGyb5ix7wmdICK7SBAgY0wwEu941UFDvNurnwZiTeYOg 3oHKUCecCo1vefPHxZvBcbQX+nbl0p2TcTQAeff0skmj/475QXVQ2C1sb4Cnyt2MgrhkRf8VCyTW CXi3NRRWGwyAcR1dD+hzCAUFuNLRggj6FuBFlVxmR0zQKWThbAoOwiEVvhiIYYbQHRhigw48OFaE tIBYISoo0CgBPyrIMBJtAWLH4VSs2RDAAoFZ9JQERYgmxF53pZGFTkNCRwaPed0AkAT+dfFFGBRw JYaUSVZJU49aahdkUEr+B5iRSD5lZpMWfHIDlGN2slYQExwAhh1NTTAAkhS8odE/g8wyyxsKiGGo Pw0kc9g6Evip41cENNBSnfZA2pwUZWHypwOBhnmYLzIGBWqmmz6KaJ+jlpoXoRO4GqliOCyKiqOd vorrK8mJeqknmmaXyB4+3ADiscw2i0UzL0Es6yyzBARL5rTYZvtCtVRsqW0cMU5gQLDflmuuBeEy Se65VwxAljjsxmuuu7akA0IEADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0068_image087.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOwGEAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0016.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td>
Aim:
Identification of protein-protein interactions.
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0016_image088.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhdAAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABABr ABEAgwAAAHh3eGdmZp6dnZOSkoaGhqenp7m5ubGwsNfW193d3cHBwcnIydDQ0Orq6uPj4wT/EMhJ q714ppSzUY4ybFmwPBjZYU7rPEywzplKS4RTAKdtPa0U50YJAgoJB4EoETiYxMaTaIR2qoUQdDC1 rqo0sNcCFq8EwPG3G2ari12nAhA4PASKISGpWAKSLhIDQA0ydCeHKAsvOxVZLk8mKHQgCF1BiwqG kok8jBSLDwMSLaMCSUtADgMDc1kGAFJ+VTkBAVoAqrktsFlzF1W6AJp0lw6wOb+6qr24ngUGkJAN jQAtAhMMUzlDVYCBpFNVZuFwpAxkxhNG4+rWDdZu62zt5fYV7O7p5sMOCQf84s27F0wcCnICB9pr Z4hfPWD0umhzAJCgu4cAuBjIgiDhPg1Kf87dS4IOjUKM+CJSKLBIocd8LgEwePCgY7yGAY/4AxCt EZAA8Li4WHBSH4VbDrAtlIHH5UOMB5TOE+PiFw4QCfxk9GcIARB04aQ9gZQuX5BVCojFOxtILFuz LUS9mUu3roVBkCbZ3ct3y6gmVvsKHnyh5oQDfwkrVrynRVYAEQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0016_image089.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhfwBvAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAwB6 AGkAgAAAAP///wL/hI95wa0Po4wu0ImzXnx7bxkMNE6h+V3pqp2Am8CPPM9lK3J3w1vO+/IhbiZX 6Rgcjn7CZPC0JGJ4IiTwCq36miEaKZtzdsNd65j8w13Xxk4bq/WqwPT3+hl+yiUycB74dkaT9ncG V2hIl7c3NcTmlhNI9vcFiacI13QoZLjRswWq53e5VZURuqCZtoqq5/TKssIYS1trK3ubq7t7KsX7 CxwsPExcbHyMnKwc07Nc1Jc76+kYfSvN4htrZ3uNIuxF2NgBy6pTBrVzR1ITV2aupJ7aLWopGGk2 3sqnYLWJtwku2xwl/eIsOjjpFDs/TCzF05YvhkRqhx6pC/cQHSZM/w+xRfQHcJIqSioSQqsoSOCH cuSMZNmhUcuUZqRYNWOiyRk7Zdt0cnPW06c2lcOoCD2KNKnSpUybOn26iyatedWSjiqJlGiyq+uW Um2hdWImmf9ejclm09GSd4vQmX3ZJyzBcwgVRekEEksUQPhQWkQFA+M+sRyNJhKLEORGQi77feUH ue64hBcJ15OckWJiiJZFKp6cd6Opz5Euk1xJJO1oMTelpIZZau2R2aneyhQM9Wfu3Yh558btO7jw 4cSLEzcKT64p5cYjZ+7Y9fGsx9/EzcOrZnAw4M7hUZa3uhPO2IY1cq91XrNJSmfXe74HSGL7b8zj 4qswl7IdLmzWgv/OqsNEgal3WETzzXfaUWl9wgxdDJJVwV3nhMJccxZeiGGGGm7IYYc2SEeddxmG GKJ6FpJojVPpURQhLJewlgQVtKGh1IoOxUYNju/gaE+Jv9ioX0V3pMTfWQ25eNxB4uU4VnxOfmTc eKTMtReDMlKInIdabslll15+SY9wFRrDVQo+TsNUmSsdcyYfFcLlSpUO3hUjQf/IiZw7e6H3pmp8 1fnSI6mFtxwn7xnKzZuQBdpkfIxCaY+TRhbE06IBNorok4lFyp+mOSHDGJ0t0hkjqeBJKeGcLYLJ GatFjelqrLLOKiaQIvLTpm9lTqfdfyOK02t3CXpVH65hllOKWYVdYonkMkAOUpqgl/ZYGpy5TlWs pX6VKs+kmXZaHEORSotXTiNhV+t+dHE7G5bkMbvhtbTWNm+99t6Lr5lS5YvLd/ziEBStti4Iq4YD DylkrAfL9y+ajTbsZnl3DlcAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0021.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> <= /td> <= /td> <= /td> <= /td> <= /td>
Targeted
• ELISA
• Immunoprecipitation
• mobility shift assay
• Co-immunohistochemistry
• Surface plasmon resonance
• FLIM
Random
• Protein affinity chromatography
• Yeast-two hybrid screening
• Phage display
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0021_image090.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVAEVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABABD AREAgwAAAHBub4KBgp+fn5KRkra2tqurq9LS0tva2srJycDAwOrq6vHx8fj4+OLi4v///wT/EMhJ q714rpVzYQ9jbJlwNBjZrVXAsHBsqTJA1zh+5/xqornd5EF8NBCCHku4GjwIgBNT0iCmOMpKIZHt TjvfrjIszlUfvKkVQFg8BrgAulxxzGNremaR1E/kfnF3gRKAhH55dHkEITgGg3qJLJKHAECEj5Uw mZqcmmKUXYmhGQFVh6QYqXoGB4emkJ8tp5WwsqCxondyLwIJDS5YA24McDZFcwZVDn0/AM4HRlAV jMjPJxICIAp3VtEMzdjQ0hTRDQYZCMYUvg3aD1zZv8ESwyHGbkUSyg/M2djXUJibVsHZuAZQ8q3h 5+/ZPAYcAiAgsmDdABB8julzNsFesX8C/8lZkEgRzkGCJB9UHPKAGxEHEuw8eccN5ASaRdAFcAPn jAEDLxgVAGDHWB4nAgQ0smTlzFBGLy7kOSMB3LNuD4Y6iUrVKZulUZ4U0GchVxE4ExVQIfIzaFai byQcfZB0KVWmb6Fe6Erk6dK5dbmyBQoAhABGQAhYTRB16qK3RfH6jVrBMGLJXylbNsISwFgsVQMU TqJ07R2rSpH1I0hEtISJ9VTKvaMwzxrbuQCMmvMAQVmsnXUDny0XpoUBvqXOUUq59QTYAJxgyVN7 uPDgv4lrp66apWsbjecsAKIAyO7nc6Rrvz4j/Hrq7rXbQQfAgFrdySnYzk/q/Hbr+vEGYP+ABBam UjzvDYfbBLRwZsEB9GWXYIHsLSghexhWcJuCA643wQgbXoihhRZeAKKA2O2TT3B62TABRghiCGOG FI6I4mx9FFiihoPkMRE82JFo3SMFMHJfBZdcuKOQLOUYZIf/BYebkx5qs0A1OPJIIZMe3gQRljZ6 eWWJdihAgCvoAenhjwn0NwhPciXHnW+mSAllhomcaR2XGSLQQANHsgOahHLw92Zc+K3nBp2XlOjm hBguCkCdHoKgnaSU+neMUXJCaWmF1n1aImIIfCeBnimyEQ0RVHrICAdjTVOFADA9UgSaoKaajXPb JeHCk3Zil4CpFiQwlCpvTcTaIK969sTkWrTWhwyujuYWonaz1jptiqymloStRqCZ7XrNxgopj3XR FSaO3nrohnFy+dpcN/JaEyBY0WG0ziMZAVBebyyphoa99wpoxQNAWSXcwfoIzPCGRZxTGbEBciNC wJTlq9K+KvXxr6EPD0xWwdZYw6/HVYBM2SPgLMDANP/ievLCd1y08WwhW8CyAC5Xk7O0Lb9M84fP DpFwH/YizIDCtzRdBj8R17CK01RXbXVBGV+t9Sc//ZH1ClNvLfbYeigQIdlolwHoBAmcPUluacct NwxVJDn33TLYc7MMBOPt998SjEeQEhEAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
ELISA (Enzyme-Li= nked Immunosorbent Assay)
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image091.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVwAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQBS ABUAgAAAAP///wKqhI+pyxsXXpu0WnelvDRyp4VAFJIjaT7bp6CssWmwisj2PHbozptfDMvRIKec MGh81Xws4HFI9ARlSSXOWsyeXMjSTosNi8fksvmMTqvX7La7vGLElUzQ2xyfJ/QVV48r1bOF46FS aBjjhZgxFXWUNURFSAN0A2nEh/H4xLkJRdkYGkkF1RfKCbpZlfqJiSmZCbL4h/iFBOEnKAXpE9uC 5+a7txtGnGZMUQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image092.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOAAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQA0 ABUAgAAAAP///wKGRICpGrcPlzMzWhPrTa62hiHfAX4iCYaUOZYqik1d2JHie9s1zcv5b1txKDFe 73crFoOa2GzmZMFWU2rJk2tdN9yu9wsOi8fksvmMFqcgTXKbvXlnzCai9XTNp2ouC3SvckTzlBUo AYSUxHSChyRHSDiktBO46CejtZfHKHW3RpeWVhcKUAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image093.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSwAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQBI ABUAgAAAAP///wKVhI+poQ3BnFwHzmbvjFSjyHkPxJFMSY4jeGZoalqnEdc2qM70jud89dPJRKbP 47c74maskk+Y1GSMS6AyeMxSq9mQqFKUNVvgcGhpU33X7Lb7DY/L5/S6/Y7P68Hq/v7eE3Xh9UcT GLiSKJaotefUc9bVOHT195IUeeVSmWJphWkkyCRZaGhaYwj5AkNY6tHqajcFVwAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image094.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhFgALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAV AAcAgAAAAP///wIehI8Zke3GzppgxsqyhBVq33WLKEbmNpLcyYUYBQcFADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image095.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhWwAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQBX ABUAgAAAAP///wK+hI+pGLvRnFQRwIlfzXgz/lkgCF1UKRplc6EWa1YrvIqeM+f6DdtHxFKpgj6h j9gaTWqPFNEJDQJNNik0pdQsoz8h8Gj8xK63LGf2Sr9aMjQ1qlOb5/S6/Y7P6/f8vv8PGCg4yFBG 54JjSKg4ssGIlTU2NJk01OMWc7nG1mbJedb19JX0lVbqqDnqNVn0+DMlutoKJkuLxKWm2oglI3tr 1RWK+8vaS9KZS2OKghbmSVNDlSlJmOFqd1391kdVAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image096.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhFQAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAwAT AAUAgAAAAP///wIPhI8Xkd27DJy0MpcmdvEUADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image097.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image098.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAgALwAG AAUAgAAAALvg4wIFjI+pawUAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image099.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAwADwAc ACkAggAAAAAAAGb/M8z/M/8AAP+ZAAECAwECAwOaCLoQHuyxSVUYA1rBdQVFuFzZyAneEhaKODoR TLkg+92qje9871eyV6oXIBA8xeOvYVRamsNdMehw3nQjII+2rHB/XyxYDJQIc7xAZ4RJkSlqFLu0 NJ+7+Lx+b4nmk347XIBjLToPgTMuXzhcNG9XjpCCfBYtHysxQQ10mINrFhidXlhxSKKJIHBDdpWu fJmusa9ijHmzsKo7CQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image100.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAwAEQAd ACcAgAAAAP+ZAAJMBBKmy224oJsxKWSplk/7D4biSJbmiaYolrJHZnJcDKv2jef6zpfuKauRgq2M 0HfsrZIijLN4YYJmyiqO6vhNnhtsFkaVMmpamxhQAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image101.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA1 AEUAggAAAGb/M7vg4/8AAP+ZAP//AP///wECAwP/aLDc/jDKZ9S8OLequ6fWJ2rcaF7lqYJru4Xu msbqTJv2Lea6x/ckGNAnHAYlglHhkozwBoIoI6BZOqiLqGAAyUG1AoIYyyhYG+dFQEwAb0GDuNwd FQMCeLPeui/gqWJ0W3JxNlxuAGMOZouMU2x0XC8Ph1pqjY4LS2l3DGCSk5QAUhF8ZacRUaChRkSt Hz+vTkWvTV20WUpMtg5PlpcZnJ2epL1FX1p2ZJrCnIAEo2BwhMhgynl9AH1meInQgtSGdN7Le2iZ d3aRxpTSicPM6HpX0NGqLO28y9qaEMLEqxjEkoWPIIaBBgXiSsiK4SwIX3RNuFeQQTVvHYSt+aYl 6yAAcYjsTNhmZWMbNwErUKuWrN6FPX8wRgp3LGQVdM/sFVMI8VdGfg2W+WzogNcNozwnsFmxdALC pidEOv34gI5LExyHCqRaFJELlL0WFBn39VNYsV3dtQC7YWsDsgykSpCr841btBYJPYAagc1VANTa CgaMpI4DuYF2OgAlxILHukb9vvUqipaBx5APh0mrtcEAyxJYpuWseDCFCCxtUa6LeSHeqmoFTaZ4 FgVEObPVLtB7OsOxVGYh9ibhOXTH0LU7OL7ABbNFsa6nOn/OnOsJhIRRRFfuejr2HU9ubZdRoQTj 8g6hlx8/IQEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image102.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCgAhAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAgAG AB4AgAAAAP///wIdBGIZaMvnYHTxpHbtfXhnrinf6JWi2Z1qyoYt+BQAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0042_image103.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSgAZAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAgBE ABQAgAAAAP///wKeRA6Wy73Igps0VoVvlTr3xoGfFl7lmJzPwx2uBEEeQr8pjN8G7u7t+tvNUiIh jKigDTFBZCsU7HmYxKeSGa06tZbktvtrUr9Q6+3FM6LLNpuv54aLJ1CU/b4iyfB8k6oPGCg4SIiy 1/dXuEHXkahIwgj5aBhjJnQm56M2GUbGdaRTBaoYJmU1miED6ojIlVcCywKE9NhWeYZbY5vzUQAA Ow== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Co-immunoprecipitation
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image104.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVwAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAgBS ABQAgAAAAP///wK6hI+pyxDdngkSzmoXztzKHVGg85UkRpGnKE7r+rBtLNc2Un2Xg+Ox6/qhgkTV yNfqAYW8Q46nC6aG02I0NHpCt87ur8kFS71LDySlurCcpdoJpmzL0p26/Y7P6/f8vv8PGCg4yHCk xJdUaEjot7EoVrdms5Z2kzQlhPm2A5doFsZkwnVlxGTVhPb1GAcWOtoKqxprFfXFofPkinu4tXsq qtUxg2l5A8Q27IZWeWm3GsnIm+G5t8xonVEAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image105.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOAAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAgA0 ABQAgAAAAP///wKERICpGrcPlzMzWhPrTa62hiHfAX4iCYaUOZYqik1d2JHie9s1zcv5b1txKDFe 73crFoOa2GzmZMFWU2rJk2tdN9yu9wsOi8fksrmbgjTJa/WmnTGbiNbT9Z6quSzQvOpI85T1JwGE lMR0YocEJyg4pLTzl8gno5V3pyhVlyZ39snRCVoAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image106.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhSwAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgBI ABQAgAAAAP///wKThI8Xacsf3JtQSZrixbVz5FzaSJaNBkhjuqAsG1qo2X6y+NbNHue88tNFUrZX hkhU/ZJMGHBY622KzyVSyOyBjFgkFbSChmYqknGXLLWm37b7DY/L5/S6/Y7Pn85Dvf+6ZcP2xwXk 5USW0zd4pyS1FSOCxVhX9mgYFXnV96eEGXgYBUhosWfitHkjRgpHyVrpalMAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image107.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhTAAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAQBE ABUAgAAAAP///wKrhBGnF7kPYYvM0bsswk/r+IHOhFhJgxreeZZsqpopLG3qbcNtVW7yPcoFKSxc sOhi+IC8pbOFzCiH0OE0Z+s9sVkJ6pf8Rl0TsbhJ7nLW7DbIDY+3SfK6/Y7P6/d8fchOx/fXp4bx lVZGMlKWsUj2YgJU9HNkhLW4k1UJZcnpqbT1pGgVucR5qRiDVHmJE+Na+kml6TSV6BgGqaV1a4aJ OGlFaHhHMywyuFYAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image108.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhFQAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgAT AAYAgAAAAP///wIRhI8Xkd27DJy0MpcofnG/VAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image109.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA1 AEUAgQAAAAAAAP///wECAwL/FI6py+0rzJu0pmizhnJ7i33iFI4md6ZXp55luwUrHAdCIAMvg9OH HYnIdgmcMKcCBm86lkIpbEGjxN8ymrwyq4ApU3odOhFeZNLWazqgZpiZ+8OlfQo4nWK/P/L6Br+P AqjxJ4hAWKiGWHFYyCjoCAjZJ6m3I6dYx6I0p1gy1dbYUYaZ2KUFGikaliGHmuHJZhErYnlZMfpB SXZKOxbDm+urgeuhuxvkuihcw1m8THpg7CNNQw1j8IwpkU3XehFNOqtjeGv7McqSbYQsgv798NnO 6/RMPHw6Vg8cl1y0ZONnzapjN/rxG+Jg2SYk8V6RWNPM3h48vwZSvChr30QQIKzEbXzFjYy5hCEZ lHRowpq1Us7oOFrpJ8gemMq06CoAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image110.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA2 AEUAgQAAAAAAAP///wECAwL/FI6py+0szJu0qmizhnL7jH0iFY4md6ZXp6Zle76wFdQBIs9TEEU3 kNM1eD3BLyhcEIs3ZDKx7DVZT0bUB6RWlcyDc3uw/bLgzbe8OKMT6rVXSxEL29DujB4uYmF4wNVY IffQ9zfmEGWYBneoB/hQ6NDnZ/fYmMi2+GgTaBmZ2XJ1iVm2VDP4CbM5IQnGuuVaVaLqtuJXSlsb iotj1LmbBfnr47uLoSssISa6Bpskger2/AvhNZ1Wbc2GnY0Njcbi3arNDc4NdAEmiL5ulYLIDm/L YzpyvF1J6RF8Homwv7EPVTJiAH15M/CvoJR7q+yJEBQORw0fy0RE1EaPz4iLFhY4KmrRrNZGjyT6 9Gj15SStRkVMFAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image111.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA1 AEUAggAAAAAAAGb/M8z/M/8AAP///wECAwECAwP/WLDc/jDKV9S8OLequ6fWJ2rcaF7lqYJru4Xu msbqTJtloNO7xwWVQsAVIBCGnQowKGwVjciMkqlc6Y4+IbXp6TWiJC0VjAkIBGTRlJm+mNEfXe8n b7vlXTbAdpss2Xx9EX9BS4IZhGt+biaJYhJFXl8BA3ZleoELT22UlSd1C5kAmxCdlmowD5IOqzKp h0mvsFKys6ygorajmLW6joa6pVvAwazDubaJxMWTSkMpeMyjUXSF0g4/Y9cMa3qTrTfd1gyEp66/ 5MOq5rdk2eOa6l8Dnhqk3BageOjz9WVGWPCVOqNDHrlK7MgdARPozZBypUSkaegF1Cxk16BZ3KZA bIEyjqF2eZNmQYw2kulOMoNhsgpKbgWdZcQ2LWE4CL3K2MTJc4TDEb1yQiLYboJQoaWixXPZU4JQ pYNUbpACid4pflPDlLLqx2DID7KgChuJMQLSqN7OGlXbbkdZWjUEvcXHFlWuIBm34DWRAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image112.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhGgALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAI AAgAgQAAAAAAAP///wECAwISRCIHJt3B3EMyUXkqNEittS0FADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image113.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhGgALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALA8AAAAI AAgAgQAAAAAAAP///wECAwIRBIJmIR3b4mPR0Yqq1e+gBBQAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image114.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhGwAeAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAACQAL ABEAgQAAAAAAAP///wECAwImhBGZZyH/AnNQyFbhpfXiPnELg4zkeSioSHIRk1lw5iH0dIcqUwAA Ow== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image115.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhGgALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALA8AAAAI AAgAgQAAAAAAAP///wECAwIRRCISYOmLXDpyRkkxo4f55RUAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image116.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCwAaAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAADwAI AAgAgQAAAAAAAP///wECAwIRBIJhJy0anEOy0XqniVZttBQAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image117.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCgAZAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAQAG ABYAgAAAAP///wIXhGOJCavcoDuyshmvfbqHDzYgMiqfaRUAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image118.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhNQALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAgAx AAcAgAAAAP///wI+TGCYwO0P2ZInIolttXDntlXiZ5RYmXjmeazt2F5ZLMYzdYIXnY6K7ZPVcrqb A1gM+hY2EuhFeaF6zyjTUAAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image119.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLQBeAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAq AFsAgQAAAAAAAMDAwAECAwLEjI+pyysPo5y0RmOzznj7332iFY7mFZwqVK5j64JpbMK0Zt/krHt5 P/kBUcONsCg4FpVDJtDZg+qkNyrNGrM1to4gD8UNf1njh1hMwbrUK7bKfYLXykhivSJ/0e/J/T0v AijDl+ZXJ+hjiIRopLjk2AT5JBlFOWVZRXcWVli42dDp9ckQSmhnSoZ6qsqIg3n1mhW7NttW+3Yb lzunmtrb9wv828qxqxdMvINsHMg8OOycuDwNTd2bjBfdaM2q7bo8Gq5QAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image120.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhMQALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAgAt AAkAgAAAAP///wJAjAOpgOsPV5NpOhtv1oy1Y1SHF5ZhR6KpWKko8rXxV7YdLFO3ja9nvJvpekLX jzQBKUWwH62J9GwimKn1ysROCwA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image121.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhIwAMAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAf AAkAgQAAALvg4////wECAwIjhI+pKLKxomoNhXuo3nyDC0qiB5ZiRB1leE7K2sYGK8dQfRYAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image122.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOwALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAgA2 AAkAgAAAAP///wJNjA2py+3fQgRSwnlxVhadelAUMnqh2SWfyHGVCr8WLKZ1HL+07KrekrLpQjcW i2c0zo7M4nLo66xWo1LUWrWiTJvZkvHdiC9ER3kMKQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0043_image123.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhIQAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAc AAYAgQAAAP+ZAP///wECAwIXhI+pJr3copw0hIfRBDbneHTeIyTXiBYAOx== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
mobility shift assay
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image124.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhKgALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgAm AAkAgAAAAP///wI5hG8RoNcIo4yLoTovrZjfpiyg+IiOhWKWp4IJ05XvucKvCT/o3NJ3vSuRQhyZ LUEkZZY7pnOpewIKADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image125.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhMAALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgAt AAkAgAAAAP///wJATICZp+2/HoNgUmVvNYhvw4CH+HkhWEXp1q1tGnbxPL71jLUx++m2j1PVVqbc jlcSnUYmJjOJ1OSk1OrCY4UUAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image126.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhTwAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgBK ABQAgAAAAP///wKmhI8Xicsf3IMy1TnjxXgbD4CfpZWm2Wihokah+75OpcYox5S5vY7r/FkAJUCe qAMSjojLplKmcP5wVEvvec0qi9HptgpGuWA6mKxFI56O4Lb7DY/L5/S6/Y7P69XhWkwPx0WF1YPD Zlh2MpXisxgkNAZZcwjRtDjWdVmomTYUyKNZqObZtTU0Y+YGGVXE1Ogo2vAj+Na69vcXOUkWxIIG uAcsjOhWAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image127.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA1 AEUAggAAAAAAAGb/M8z/M/8AAP+ZAP///wECAwP/aLDc/jDKZ9S8OLequ6fWJ2rcaF7lqYJru4Xu msbqTJv2LeZu4H+8VYBACABhmEJmSDR2gpGCEjN0PpHJKePnsF490q5A4MVhP4FxeQTV+Li1s0v7 1QHCR/udTpIv1g2AC3geQT5FED4DgmYRTIABA4s3hk2JkoxsfnAPnC5tehOgoRGjpCwrfCemE1KE e6oofmB0r31zDrYZrKcMvL0Av73Cp8SkxqHIelCejpmJa4ZqF5GTbpYvjtMT1c9bRIjZ0M1dbx1V ZcpZjhDqra51dkq6EgqzwL4L9/jB+vwo+WjFwrFBBD2CAcFQcrDvGIV/DEuZIEeinog0ZNgA9IDR L9vDjefMAdklQaSMJ5cwnSyEpJo1TQi3UCQZ51PDXTfb5Yx361iFWT8h/hwa1EQCADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image128.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA2 AEUAgQAAALvg4////wECAwLjFI6py+0szJu0qmizhnL7jH0iFY4md6ZXp6Zle77wKM9fbW94DrK8 7ksEhsTfykEsfpLDyQ7ADIiiziCCumRWkdpscmuEdh3PMNlqfpTTizU74X4f4nL6287Gp/Vm/gHr gkYBGCM4QWjiJ/bF1QTmgSg01qCYEXkUdgknOLmhifD0edjZxknKE3qak8rYZ6q0Zyg3RztLVmsL gZt7scsL6gvZehPsqUoCZyKqljyyfAvsfOzUOzWsESQbhqb9013HbCv73dJNHgOyp7NNjEqzWlh+ rjaPORNxju9qqD+L/99PRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image129.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOQBJAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAA1 AEUAggAAAAAAAGb/M7vg48z/M/8AAP+ZAP///wP/eLDc/jDKd9S8OLequ6fWJ2rcaF7lqYJru4Xu msbqTJs2HQRf/gxAT6BQ4HV8DaASYMgMicYMcqGsGpqXXdEzBVSV18dO3INBvkwsIyAQRHHmczDC dsvi58luLJfiY01VGF0qV18DKH8tgYcTXW8QkA1haI6KQ3wOOwSSP3OWEk+dAQScGYiTYS+hUJGl nRiqq6GwAJkfsgyEN4OKvH4TlSaoiRKNEmpyxBJTxxBXyWkGwhHN1F5fuYzXDtaCSdkOlN/MvtfO yr3D3A7L5evkNb4X8fK/J7v31fP6+3q1DW5h2NPtUhuAtl51EDVLTBs7ehQ6IbJF1yWCEQXqKfIm dt/ACNECstAH7Qi/E9NCvtO3hASAkyLYUVgA08MhdxAs1Oxwc5CuXzI3CAX6ydFQoC6P9mOW84TG I01H1EFYjamIqSLm7Vywh+pMo/+8+hzrypQMk2XFWuUS6SmJrT9vwC3owqM6fL/s0tRbpkuFufj+ CubbIAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image130.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhKgAYAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAm ABMAgAAAAP///wIzTICpy+1gzpuUxYrrzbztDiJf2I1kZp6aoZZsi77wKs1Ualty7ow1L3r9gEEc MDI8IgMFADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image131.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCgAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAAAF ABMAgAAAAP///wITBGKGuZr3GmSx0utO2Jzr5WVZAQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image132.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhKgAYAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAQAAAAl ABMAgAAAAP///wI0hI+py8EPV5ixvumsRnh70H1aKFZkCZ1oQ61p67JZLMF0ot5Gvs6gfcvwYhig rqhTFI23AgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image133.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhdQALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAQAAQBr AAkAgAAAAP///wKKTAB2yN3mopRrwgunjjlVznwbImrl6CyqiT7u65HkcaUZTc8epvT5/lslQj2Z b0gUHoe5VU3GZAabxlDsZnRGodSrNaqqhJHBL65M1Val1jSG+xSStx3xXK6+g9f0kh3ZBjUHqDW2 A3dYpwCEw+jDA/lH2Jj4SHn1ttiyyTki2QkaKjq6oUl62lAAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image134.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhkwBeAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAACN AFoAggAAAAAAALvg48DAwP+ZAP///wECAwECAwP/GLrc/jDKSau9IOvNexhgKI5kaZ5oqq7sGnRw /LV0bd+4+sa8NufAoHA42PV4P6JyyRwZj7Bkc0rNPaEcaXXLTV2xvq54XPqCAVqyumoGp9fwZRv7 jtuDc2j9zrflj3t9gi48BYCDiDdmBYyHiWwXEyRXjJWOj1ORkk4blZ49gZhWmhGTHZ+goqonbY2p q7Aif6+xsbNItbW3Mrm2Zx69sLtRwavDwMWirYa0a6QQgl+oHAJhcc8PLR/bRd3c397dZZ2elhoC 6BmhVNgO0afm5+jp68nOMa4Z8/v19mS7++b18ydm2D51BBMdS4cw4aBjWRw+/BVRYh+IGwZazEQx 4+NGPhitfbzWUeTIeyXRnCSZUuPKISEbvvyXUuVMmi1v4izpUieOmDZ9cgHaU2gNokaH1iyalAXS pux+ESBgEqocfMw4TJUpqx2DptLiaZhKNWhXrwoegVsbju04DeXEbuUaAu0CsBzKXbJ6FV5WXnyZ zPlLLHBfnoYPd2Sa2FTOxjCXQo78eDKQp5atSM6suTJnP5s/g/YsWlvo0qZJo9ZxejVr1a5ZtY4t GzZtx4hve5mtm5Pt3iAwA8e9eLgJ4cbr8jaOPHmR5cObJ5fOHDpw6tGt98Z+XbtuonbDix+vqWYC ADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image135.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhQQALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQA8 AAkAgAAAAP///wJQjIGpi8EP34EOVBmzZpc3c4STA5LLSJYWmFTp6lrNHJthi8u2zO/2ONN9drxi TRjTAYs9WlBozPmaUKQLNYSpJquPtoRKiTbk8smMTmdY5AIAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image136.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhLwALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAs AAkAgAAAAP///wJAjIEJoe3fmJLSVWjxROws44Ed+H0Ud1BeubTJebYw2rn1/HK2rqr8j9PJhDUX ircSKWmmYzNFWmk206o1Ir0+CgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0044_image137.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhOAALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgA1 AAkAgAAAAP///wJMjAOpy+3fUAQSsmoXztLQ83kIGI6g6FHJMSqVuMYt1ql2q9bwnbvdHuvxfrpg seZKKnFFm0yWYpmi0xXqZzWVtJYXZJMJc1IOkjhTAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0047.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Immunohistochemistry
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0047_image138.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVwALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAMAAQBQ AAkAgAAAAP///wJohI8XCbEMzYu0uXormpHrD3EeM16mtWRKNjku6zZbmqKmCsc6Lbeb1JMBaz2i hEdMClnL4MOIHN6GzaciiFU+rc6s8FtN1LRAqvVMtZS/L1pbtFO/3+rjrl2vv9agvv8POAI4SDio UgAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0047_image139.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhbgALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAMAAQBl AAkAgAAAAP///wKChI8XCbEMo3rGISqjw8zm+3FfxokjqITdYrFt6VHbPDVbGlc09jb2fdPpgL2W r4Ji+WTLo9IpDLqOUeXjib1CrUimcdlrYqHiptm77aptyPKYmTLjymc1t25fv4VU2W4WNDT1Asj2 M/RDSDRBVOhhSEN1MjlpQnl5aYm5ecjpOfJYAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0047_image140.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhHwALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAd AAkAgAAAAP///wIrhI9pEervGgSMskptbZvflUlcBlrkGJ7bMYpp6pYwy0rkcnm6PPW0DzQZCgA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0047_image141.jpg Content-Transfer-Encoding: base64 Content-Type: image/jpeg /9j/4AAQSkZJRgABAQEAMAAvAAD//gAcU29mdHdhcmU6IE1pY3Jvc29mdCBPZmZpY2X/2wBDAAoH BwgHBgoICAgLCgoLDhgQDg0NDh0VFhEYIx8lJCIfIiEmKzcvJik0KSEiMEExNDk7Pj4+JS5ESUM8 SDc9Pjv/2wBDAQoLCw4NDhwQEBw7KCIoOzs7Ozs7Ozs7Ozs7Ozs7Ozs7Ozs7Ozs7Ozs7Ozs7Ozs7 Ozs7Ozs7Ozs7Ozs7Ozs7Ozv/wAARCAB9AZgDASIAAhEBAxEB/8QAGwAAAgMBAQEAAAAAAAAAAAAA AwQBAgUABgf/xAA6EAACAQMDAwIEBAQGAgIDAAABAgMABBESITEFE0FRYRQiMnEGI0KBFZHR4TNS cqGxwSRiNJJUovH/xAAaAQADAQEBAQAAAAAAAAAAAAACAwQBBQAG/8QALxEAAgICAQIFAQgDAQEA AAAAAQIAEQMhEjFBBBMiUWFxI4GRobHB0fAFFDJC8f/dAAQAKP/aAAwDAQACEQMRAD8A+M11dVo4 nlcIgyTXuk8TUhFLuFUZJOAK9D0vo6zwSZhVnXksM4pWztvgVN0UErJ5/SKbiuisXxSTaGY/R6+9 Q58jMKSc7xGVnFY5RbC3MbRFIw4GS2kbe1dFZWseC8QbbyvNXVhOO7cFUXVsEHNNERknUSqLsueT SS7DRMmbIw0TAra2aoWeCIuw+RQgxQzZwCUkwxaBz8owKief5iIBpA8nxST3R1aFzI3vwKJVc94a I53cPMluW7cdvHt6IMmqRWCzHV2lVf8ATT3SpYohIZ1yzct6e1af8RhhjHZTUF9RxQPldDxURb5n Q8VFzMsbC3lZy1mCAuw0bn3peWyR7jtLbIpz4UDFPSdVmlkUxZTB2IHFFj7hLSM3zuclmoObqbMD zMink36xT+HwInb7ERcedAqhsbdAS1vHuNsqKvHPd/F5WABRy70a5lkzrGXPjUNhREuDVzeWQGif zibw2iL/APGTA2zoGaGlpE41tFGiD/1FcDLO7IilieTiizRLAVW4fuuo3HAWnAHpe4+yNXuJpbC8 nKQxIqjk6RWXKuiV19GIr0trJlSyroU7agP+K81N/jP/AKj/AM0/CxLEe0q8O5ZiPaVrq6rKpxq0 kgVTLQLhoLVpWZcYIXNU0AEg4GOTVkmZcuHw3gVVnLsWOM+1L9Vyo+UFFDcrgDxU4X0Fd5rvtWxd TtIzwK4gcYH8qjVTNnNHC7GVNWRyPFYSQLjMaq7BSa+YuF34/wBqssZYkKhJ9MU3JPCMFF1ex8UG S6kZgy/KRxihDMe0c2LEnVr+kEy4OCuD9qjSB4FXeR5GLucsfNUzv60xQTENxB1IwPSuwPQVJqKy CZIUN4AHrVeTgAVYDYajt6VyrqbbYevpRlSByMzRNCCPNdUt9R+9cqlmCqCSeAK9EHUivU/hH8Nt 1a87DwhnZdQ1jYDFZ/TukSdwyvF3jGNWldwuN961rPqZn73UFufhJoSAiLw3tXP8VlZkK4j9/wCk hzZuXpXp3Mo3RobS8ltbmKISFymSoIQetBh6TZxHMgEgB3bTt9hTEl/J1eSSW7aFEVfmkUYZvv6m mlEDLGkZZINOdT7FqlL5FFMd95z8mR1JFmBitOmgGae3h7e4RVjG596DL0+B3UrawoCMsoQbCpvL lI3CWqgEeW3/AHrKuL90bS7GWQ/pB2/ejxo7bBm4seRzYJjt4tlGBHDaw/MR8wjBP2FKxdNNzLhY AoUgHCcH3rW/CV9DZdYF11CATJ22AUHGjPnevXP+IOkRwZs4DKzPqaN0xv4OaRm8RlwNwVSfmPP2 eP0tueQ6X0iG4v1tzYdwKDn8vdz7VTqnTY4roQL05YdX0hosNitfqf4vub35IUEOlsjtqAQfvUfG X3UZ1urudppgoVJHHAoRkzg83FfFyV3KEuSZnRdHt7eIpLbws7DIygJFV/hVvHhpLWMqcj6AMn2r rq7vx1BY4bYSkH62G33+1PXdxK6ozkzEcqi/KT7e1EWyCrPX5ii+UUSevzMs29lGuDaRZ5JKD/ag RWUNzIdMESRLuWKAbVMs8810yLHrkYcKNhRZbOS0tUF7KWDHUsS7Y+9Uixq9mVAlep2ZmzxJPd/D WkETHjUEGK6tfp8od2a3t10pycbCurHzuh4qJ5/EZVPFB+M8nFE80qxRjLOcAVpvZ/w8IGJEhOH/ ALUSxt47NVlk3mZcqBuRQb2K4eZWcEeinwKsZ+bUDqXtk5vxB1+sdurgu6WyIyoBlwRsaXudEEOZ MF2+lB4FXyY4GcAuw/Vz+1LRW7Sv3pd996WgA+kSigD4H5xyFHSNTJpYg5Aq11eBmUHJz9Rzx7VS R0GlRkn78VRYiZDrYYH+WgoE2YFAnk0HI7SHC7BuKJb2aqMscUURDWAFyp80RZNBLMONhmvFjVCe ZzVLOKJAm4z5FLS3LSHtodvNUurl5X06s/ambKzIVWI3Nbx4LyaeoIvJ+sLbqoQDtnWfWmjOqLpc HPB2zUoio5AzgDOfSgu/5h3GPU1MfUZKSHMsziQgHYeP70NpGOqJWyDy1MQ2q31prgGl0chwTz9q JGspU21tCPiAMFm4FYGUGjB5qNe35TMPUPgfybUfmvyxFEht3ibVeHU77hOf51ZLS0sZAWJubn2+ lT70xFDKJDMzKWY5JPGaryeIHDy06e/cxrOoHp/HuZMwDAKqhUG+BtXkpv8AGfH+Y16meZYsyaCA B/P7V5ZzqlZscsTit8IKuV+AB3K4IGaM8pCKi5GRvtzQm1cHP2qckgZOcVYRc66sVsCQeK4A1wGT k1bIr0wC9zs4qCc8VJGDuQftXVkLcgDFW4qK7c1oBJoT2hOzUgbVAFX8VhhAXP/Q+PVNdRVSFrZ2 LESg7DwRSyaliqW6QXnFSGEZ3GT71XJB25riSTltzVGJ1x+rvFNZku2o1OsBdKjGefeq4rsjk0vI 5yNyaavpGoMglsDkmtdukP0+zNxOSky4IA3AB9/Wq9KtYVxe3JGkN+WvqR7UXqy31wgkdXWInUqs MavepHyEuEBod/4nLy5S2UIpod/4mhcdXktel29rZrIkswOqQAAOp2I96Rmjjt7RpZiAMYSNdi3v U2aMkOGzIyrwNwgpEW8t9M0smQgPB8Ck40UE1r3+YkAE1eh+ccsY5ZIS76VjYZVPanLzqX5CqV1O NtIOyCl3eKCAIAx3wADvQ2gZ5hl1CbMNJz/OsIDNyaKKh25N0lJbjWB2s6cYzjzVrLp6s/clbcnO eaYMCjBjUuDsTTClYpfmGI0rDkoUsxstLSSUt4oIzIyllPqaSn6ic9uLZjyR4qOpdRkcaA2MnYDw KnpfTXlj7jjfOQf614JxXnkmKgVfMyxqxSMR6pIiX5DE1qLcdhdEoK4HB32NTFbJGyrux8r6e9Cu XzMulgR6nxUbMMjSF2GRpL3KyDQMhDyTyaE9w8DmOJtZIxkcLT3Srfp3VLa9tSzJfooeF9XyMM7j 71NvG1jILb4TuXP6Mn5Sfel81BK1sdv3mPxxj3Pt+5mJJ1NOjMZYhrvH21HxUxW99O46h1SRjHL9 C5BLn0x4FMz9KtLS7e66s/xF4z5+Hh4X7+1MLDcXU4uH0KQAqoPpUeK6DeLC4imPoepI2fgfH9Ea cqBbTv1P7D4+Yd2T4QR20SQh1w/jNdQrhxrB0BSv1P8Apz9q6oFsDUSrPWpitC0Uqzg4AAK5+1TE Hv7kl8kjc7c0GHXdFDJ9Ax/KtCaSKBAiYGPI81axI13lzkrrvA3jRwLhEwRwBxWXJcO7EAfvmrzz lywXg1SOLLDUNh6U5FCjcoxoEX1Q8MS6DrY8ZJxRogkjAAYVRx5NFDDthQMYpXXgs2cLnn1oLLXF 2WuGdmdsA6EUZx60pcXK/wCHGdZ8VJhuLxsKcJ4zUTWy2u0Yy2caseaNQoNXuMQKDRO470y1tmjE kg1yHlSPNaDQokihCNuQDxWZiQBSrMCOcCrQJIsmssRp8tSHUsS1ybIpYluU0bqKaNzGEKIQDnk1 lyJK03bCk559aaa8llXtqWY52JPFHisjApuZZGKt+k/qoFPlj1dYCk4h6usNbQNZws6DU223jJqk 9w0AYA5nk2IX9NAlu53cCEYIHA4FLNfLaNrmhZ3P6v6UK42Y2dmAuJmNnZjlrBFEc3EgQHckVN1d wx7QYZOASfFKkfGxi4kkVQeUB3pS4Ys3biXjwKYMYZtmMXFya2P8Sl5ctKdOSfArKKkHJrTgEa3I ViM+p4rOmJMzjOQGOP51fioaE6/heItRKli7ZO9dip2FQT6U2XfWQTmpGAKjFWFbPCRXV3mu3Irw rvPSCfAosaRsmS3zelCK4qRXus1TR2LhGVR9LftUvGVVW3ww5of7VbUdOM7VhFdDGBgbsSp2OKkD AzXY3zUE16BO4qQvrVScb4qc6gK0Ad5lyTgDaqtXH2qM771oqpjGbtlZtJ023nBwqk5J4zmmnubj ql3HA5Jz8qEDwKQ6c09zaLb7mJGO2eN69F/4tlaKqaQzDZ8biuTmbi29ndfE+c8S3ByOps18QN7H b2lvpEeDxgeT71564vnaQoFH9KYvb4vM2jPvScURkYFgCGOSabhx8Vto3w+LgtvGbKHX80jHJ84z imo+0zLDHkDO7MOaYtiscPbVcZH7mlZHJnfTgKvL/wBKEsWYwCxdjGJWkZhDCTEvrSF5dpApiVw7 H9PvVZEur5u3bkhByT5rp+mLYwanXXLjJPOKNFRSATv2jMaopAY79v5jn4esrG7Zpr9yJVbCxsux HrXobiytY0Qw6UY/N29W4FeciSY2yMC4kIzkDBq6QXMs4ZnYktnLbAVNlQu/LlQ9onP9o13VT0t9 ZXVtbwzRQsI5U+aQnNecvknSVY49Umrgkb063VbhY2tlkeQeBqOkGj2nSpnHxs07KsY+b39hSMd4 Rb1/MlUjD6m/+mG6V0w2qi4P1hC2kcnHiiz30lsfjLjQtwR8qLvj3pS46jKVEVuoU5+VRuT96Qnv mtX799btM5PI+kUIxPka2iRhfK3J9327x61g1ym7vHEfcO7EZI/amb69sbaLNk5lHJ1etZaz/wAd gabuLboh0hS25H9aRvGSNBBAhz43yTTRh5PTHY7do4YOb052O3aR1DqbSYGo49Aa6kGVIpkWb6iM keldXQVVUUBOqqIgAAmjCezEkBGGIGKoYLi7J+dVQEgHO+KnufGMDpIhUAM45I9q0LTtC3ZiMhVI Axvip2YpvvJHcputzHFuFwqnVg70Z0KFEXBY+K5giZkRsZ4BrkLSQsNIAJ3byaYSTuOLE7n/0fnI cRnMrEjwo/VT/wDDjcwJIGALLkJ6CkiqaQBgso5NVjMryAo51LxvxXKYE7BqcVgTtTUdtTDJKLd2 0rn9I5ol500oSwYMg3GDxS4hK7EAeSaeh6hb/D/DSqdA2Dj/AJpLcgbWTuWVuSbiUUgQZIyxG9SY 3mbK533I9amWO3yrQSZHkHxREuktFLo244NFvqo3CJ7qNwiwR2mGlYMedH9aBcXvxI0j6fTOw/ek XuXurg9tdK+p803HDGNKyYb1AOBWnHxNt1mnHx2/WdDdRvJ27eHu+rHZV/rTN1GGiPcXUv8AmIzn 7CuLRI2UwMDAVRwKXkn3DPIXznYUPU2BArkwIEQeDQNcakDznmq2kkUU6vI2FzvirSvgHUwCnfFI 6WlYrGDj1qxRyG50FXkpBhWniiuXKjvMTsSNqRbOokjGTWnb2ug77Z5JpKQGRyEBKKdzTUIvUv8A CBWYgdZSOFpd8hR6mqMoVioOcHmmIWTZT49uaDJp1krxmmAm50XRQgI6ynFcOa6pxRRMahsjPFqV xq8KaGsSrKY5mKEbbDNDEjLjDHY5FczMzFjyeaCmvZlJfFQpdjr7GNT2PbXWrhkpTinLS7SNO1Km pCd6rcJadovDJ82fpNCrMDTR+TFjdfMxED3F/pFa7jmpFVbOcU0KSLkJIEsSunnf0qMgbc1GKnAr QQJ42ZGKjSBuKtmorJhAkA4YGufAY4bV9qjniuC1sCyRU3+jv8LYB2U4mYhTjzTYtL7qU7W8TxpG hALs3H2pfpskt506CytomYQ6mmcDOgE+PfFej/D3wS3BhBJhRsplcu/rXG8RkOMs1bnDyAJlLsJ5 ebpy2s0kImEzKd2HBojw9m3UgAF+F801fi2a9luIGKoG4byaBDJJL3AqBiRu7ePtTQ7MoJgu5JuV B7TZnlKKOdO+T6V6LpH4eXr3TzLHOluclY4mH1n78VgLFGkYjY6mzneqLJMziKOZhhshVO2fWl5V Z19DUfeYnDlbCwJsQwQ9P6k3Tb2QwKp0u8S6iDW11b8GA28c9jdpPbMuWBOD9zXnTHO7mWYAyyHL Nnmt/oH4mtuiQy2FzAZ4ZWzIQd8+mahz+cKfEbI6j3gY2wlyK+/+Z5+Flgf8w5KnSoHpV3D3JymV J29dq1urQfhuW2mm6bdMLjugiJgfmB5rPt54LX59YbAyaauTmOQBv5EV4jEcTWNn4hILCK2RZriV VLfoHJH/AFXXfVo54mghXtjgRqf981jX3VZeoXemCMjP1ORz9qagt0VMzENq5VNiaYcJFNk6+0Uc PGny7PtOjv7aOVYoYTczfSUXZc+7VqvGz2+JFR1IAO3yr7AUP/xYlRYwsYXhF5J9SfNK3N3qYlpy QDsg8Us+sjiIkjzCOIr6zKubKPuPJbIRg+dv5ClIZES5EsjEaWBOOacuZsuzhhGjeDziseU92Qrb qT7108QLCjOxhDMtMY71a4s/4kZ1mN0rjIGnTj2NdQrbp7IdbDLe9dR2igL1qML4xrr98cXKwZwV Rl4o9pMDFK5fTgYH2pS4MzHQH1qQAAOBS8kirD2cmNx9WfNK4chE+XzEHNdET4XdfWmo7rMe5Cis vQS3y8UUxSsm+MVSyLQlTY1oCOG4MraIhv60/DE8UCs4A9vX70nZXCQQ6flDDfJG9dJfFyAzFs+t TspJoDUmdWJ4gajMkxAw53PG9AVyzbjbwKqPzpRtsds+lGaKQIVjbGNs45rKA1MoLqMrLaREKI2f /NngUtOq3U4WJSI/ArhFJJpj5PnHrUSmRD2YkweGb0oVFGwdwFAB0dw5hjtmCqwIP86tbus8/a04 LA6SPFJNEC4Afcck1cSCD5oyQR+onc14rY67niljruNyviP5huds+azZ7tVBVM6qPJ1K5uAYUOoE 7sV4qsNjpk2fWx5PpRIAo9UJFCD1xaO3keRTKNjuFp4QJHjJCnO6imO0qv2Yj3ZFGWfwBSl46g4U HP8AuTXuZc1PeYchoSLi4VHAUAD0G+ayXlOWC5UH6hWpB0+5dfiHHnA1bAe9ZEgIlYZz8x/eqMQX YE6Hg2CkhTC2wDOSW04G1BkOHIByPWr6lWPTuH8mg49KeBu50cjegKJcGu1elVwasu1bFgmSAa6u 1V33rIep2aKHiUghCSPBOxoVRjasIuErFekJI6vJlE0KfAq0kUYUNHJqJ5B5oJ9q7FbugLnuYJNj rCxKDKEYc7fauaJkzkHY77VQEg5B3Hmim9nKFC2oH1GaE3eoxDj40/WAJAqME88VbG+TzUhWOwBJ oojiTP/S+PYxXGuO3NRuawV1MoPsJv8AR5JE6fqi1RqdSyY4ffzWh0K5DdSjAm7SxKTrA3zWPA9w vTYVjk+Q6vkG2+fNck0dpaywyq8VzIflkJ2ArmZMfPkPecUpzyEt7wfV7zRfOsUglGclvBNFs78m LBIXI32rGZMuQmW96KsM7x7EBRVZxJwCyh8KFQJqSXwdxHEA7nz6Vo9PtJ1gMkmFyc5xu1ZPSJks 2fuKupvLDNNT9YLatUjN4A8VLkRr4INSLLjYngg17x2W6ZPmkwFP0gHikzLqkwMlPQHmgvL3yoVC wFOC2eMaYWwcZzjNDxCDcWFVBvrNCKfpdrFHIbeSS42ypOUPrmlutXFrfzqthbfDofqRWzk+1LJF N2hE51SE7kelWn7lgoijgLTHbPpSlQB+QNn6zQ1Ggd/dNCTpllYWkc0FyGkIGuN9mBPOKraywN1O G3uI2ETMEJXdhnyKyHgZgqPITIdyT49aKpW2w8cjNIN9Z8fatOMkG2smEwViGPWbt5bS2RnjZNQg bTqK4I9xXnbm/igyBnUfXetE/jHq4ja2Sc3AkTSVkQOBWcnTGaUSyS9yZt2GOK9gxsg+2/Lv+ULy 8SHke/aICG4uWEsmpYnO2/Na6WMUS5YCL0A8inorRIu3AoNxcyjKIOE+9LdTIhj0SB+6PqJGP2FG cxyMFEU2ZspAXQgby6jgQBAFxyea6lrXo971ENMyMY14U7A11MBwppjuMBwJ6WbcEk+nGB81RLbm ZzIyjJ5yaLbxaV14GcctRdQdsNxRFqOoZaj6YisTE4Awtc5WMhQc+taTLGLd9A38seBWWyqZPlIJ /wCaNG5Q0fn1gm1SPhNqsIJB82cgVo2dmWGorV5oDrIwQPGK05hfETTnF8RFraf8sayF3wAPNNB2 ZcIc70lKsULh2ByTj96JCXkIGvQhOTjk0tlB3FuoPqE1Lc4wEHP1N6VS4UaSF8Hc1wZVQrnAA5pG 4vRHGVjcYz+5pCIWbUmRCzanTzrEu2P60lma5bCg4J3NdD+dOrOCUzx616u3i6ZOj9mBkfRnGds/ an5nXBob+Y/LlHhx0uZFpbJGmld2O1RLcLG/Zjwo8mj3eu1IhVfncb+1Dt7PUCSupgd2PApXIH1N FBgfW0es54IU0FRljkN5z/Sry2EczPe4QlckjO1ZDqLaRiXLSZ8cAVFvL8ROdblV43NAcR/6UxZw nbqYa7u7iXSijCePQVgMcSN5OTXo7q9VYlVwdMf+9ecYhnZgOSTVnhhS9KnU/wAeKvVSH+Y5xUYr s1OKpnT6m5U7VxzU6cnA5qwHtuKMGhBomUwa4N61Y81U45obueIqTmpqtWFZCBnVx2rs4riwYjbY UQUUSTPE7qV3PFWAArQjFpcEIsTLJoPnbNKzQvAqlgPn43pQezUpfwxReYNj3ECdqZtbhIgVYc/q xuDS2Kjg0RAIoxWPI2NuSx8WxvcyKVDEk4z4FJsCBwa6JvzBlio4yKvLKxVYiflWsUENUc743TlV H9f47xqzuRHAoIyRnG1HuInvyjyRjUBznmq9Lg1xiQgbZwW4G9PM+SEc7e1SZG4ua6z5rK/HIePW ZQtnEmiNcDgmplC26gZy55FbsCQBX0KXfT+w/esG6VWnwGUkHfBrceTmaM3Fl8xqPaKzOZHCoCD7 VYWcxIGeOfatPp3T3llJZBnPPin7qzKnGlgAN8CibxAU8RCfxSo3ATLsbgorCYhFU4z5NaSTlkbt NuRzjis27ghhxJKrZB396tDK0o/KftodiaW6h/UIrIgcchNyzJTBVQZ/II2UetGukU6wp1vyWPig waYowoIIO5JO5+9I3PUUt+4I5VyfJ8moghd9TnjGzv6YO5nSBDk/c+SaypLme8fEKnfahl3vJstn STvX0/8ADFv+Fep/BWLdPkiuypQur4De596q8VlXwS8iC307Tqqgx6/9TxXTLBITlmzJTN3dLZMY kCrIfrY8itnrPSp+iQG5aNV7khWNQwzjwcViWvTmnlZpYzLIdyBwB71OuVcv2hNiQvfLnm1/f0m5 +G+q2Nhl7uMSpOuHkwNaN4K1r3HR0/Ft4ZlaBGhACDVjVgbkivE3UEdndFnkydPyIm4FUtb4z9Qi R5ZIUB+dwcHH7Uh/CFmOXE1GusbjJet2v4TQ6jf3sSPa28Z7asVOjjI966mbvqjWvTm6Yj5i1ag2 xznzmuqjB/z/AMiAqoulWZM1rphV0kVsKMqPFUtrcTI2o4YHj1oLXDyLGsa6Nt6rKCTqJOw3OaeF aqJhhWqiY3f2bR2quzjtZ4B5NZ8FosrF+BnirwTmSRIncmPPFPGGGJmyT287aTW22McSdzeTYxxJ 3JtZezlSOeGoodgpKHWzHjnFFa3glhUw3CjI3VzuKRkglhVtJC45OeaQKYyYFXPzBXkEMsmWyXG5 AoaR9sZC/wA/FOWYTT3HVtQPPigX/VTGGSNEIYY3FOUsTwEoUuTwAuf/0/lt1fZBRNvWk1VpGy2c VeOJywkKEr52oxaVAdEJwONqnFKKEkACClj1sEjhyY+PNUjuSsxEblGPkc4pH45wSG59KP0wxtKX kNJOOgWaJbEQCzTZiiWT5520nAAoFxdlQYoGznmhySNO2ImAjzgn0qjxp9CZGeSeanC7syVUF20X GvJydvWrwx6iSNl9arJkNpA+WrTTIsYjjz70/Z6Sg2ekYzF2sSqCoPmslrdWVnVxnchabUGVlDHC L49aRkmwCirggnejxqQdTof48Ipbn7SiRhsgnBAyKK1qVtu9qHOMUAnNWjkwQpJ0E7inkHtOijY+ jCUqw2pq5gjSTUp+TA49albeCRMpMFPo21Z5gIuM/wBVwxUVY+YpkVVhmjtaSqTsMDyDsapH28HW DnxiiBHURRxtfFhX1gwK5iBt5pgXfazoRdxjcUruTmtFnrAcKoAU2Z25qykCq7+BXaiK2JBqXVyp ypIPqKnJONRzQ13NX+1eNRisanE1FTUcVk8ZwPpXc812QK7evTJ6/o3R1u/w6lwl5GsoLnsnk4NB sLP4m8aGc9rIODSth1F4+ix2sMQEncJ7h5xVpQ7oqu5L45B8VyiuTk1nqTU4mYKGofM35vwzN/AZ 7tLmNEj/AMRVYE4rykFhFdXBxkKuwPk0SLqMsYMCTN2nI1rnnFejn6bZpHHcwP8AkPEDIUb5lb3o A2TBYc3fTULgVxkroj+3ErBvgZNl1LxnnTTnd1SPL3NT+E/zVqWnTOjdQ6fm16kttKp3S4IAYe1Z V10C9s537TRyKQcTI2VI52qUZceRyCaPzIW8M7+qv3iHVbeK5UC4JEux0ikYbURE6Ij7A1p9JSCW WRr1ZXIX5WTnV7+1N334s/hMitZ20DsmxEighqpD5AfKQX+QjsINjFev72nmr3qfbQxIPn4J9KyC XmbJJPvRX7t7cyXBjJ1uWYKNhk0xmWPaK2YrjOSK6igYxQ6zoqq4hS9Y/wBLjiSI6ocgDOT5ojdR +HvVkt5GikB+Rgd1PrWT/Ep1bDDTjbHFE6e6XHUQ0xwtJbEds0nbCQTkaeptw9723vJmKjOC5yWJ 5Jql1fJY5jtZWZ+P9VKXFz3WMNm64A9ePehPFFFGAmpnOxY+ftUIxgm2/Cc4Y+TW/wCETczPKxbc k5O/HtVoYzLONGTj6m9K6cGLCrgjG5q7XENvalFB7jDeq91qW2a9MbhKBHjf5h6+v711ZgaSVBEr aVPJPNdQ+X7mB5QB21RjHbQacaiBk+lLXVyB8g//ALSr9SLjHbwANvmocV2qSl3i1j01YqhcTDZE qXAw2RH7dY0tjMzYcH5RRGn7hQgEjz70g3UVOAsGB/rz/wBVC36qc9k//f8AtXvLY7InjhcmyJqZ 1LqJx6CixKdBZ2Okc+9Zg6uP/wAf/wDf+1Vn6s8qaRGE8DB4pfkudVFeRkOqjV9fOqiKFtOf0jxQ Le0aRwZCdzvScVyqPraMufdv7U9/HNKFUtVH3bP/AFTSjqOKCOON0XigmmUjhQIGIQcChyT6U1Ow x524rObrTMukwDb/ANv7UtLfGVdOjSPvSlwMT6olfDOT6oQw/ENI6qMDzQ4lZnEYOBnerDqASAxJ DpyNzqz/ANVS2u0t8kw62PB1Yx/tVRujr6SsB6OvpNeICJlUEn0UUW4UoPl+o81lL1XSRiAbf+39 qiXqrSZKx6SeSWz/ANVN5LltyXyMha6nXs+H0IdxyaHbSkvhzz5pYvkkkbmo1kHI2qvgKqWDGAtT aZlTBBxislzl2PqTUi5cDcZoZfJ4oEQrCwJ5ZNyScVZQukknfxVFcBgSuR6VLSKfpTT+9MIlQYdS YQyFkC7581Wh6vap1+1eqacl9YQOwGNRxVS2Krr9qjPtWjRuCXvUsATuatVNftXa/asNmeDKJcVV lHioLmu1+1GKCn3mFgZwBFWG1V1e1dr9qxqvU8pAl6q3FR3PaoJya1aGzPM19JwO9XJoeanVQkTA 1Tc6cAnT1cAFySFz43qLy7EUYGRqxufJrOj6g0dusOjZc7hsZocdyBcpLJH3FU5KZxn96l8k8ixn P/1yXLNP/9T5l0uG2mWaa6coEQlMfqPgUy9/3bRIkU5B3OdiPSs+bqkDjENl2xnO8uf+qCL9A2rs H/7/ANqiOJmPIiRNickmptK/cGD8igcCjWglY7SMqYy2/isgdbAwBa7Dxr/tUy9ed00JAEXG2G/t SjgyHQEmPh83QCo91LqjWsBjt3CE8KOT71kw2s94/dlJJJ5NLJMO5rkUyH3atSD8QrbRhY7Jcgcs +f57U/y2xrWMWY/ynwpWNbPvNiO0gs7fCOVDD5/NVachGIIKLwCOB6mskfiJ9BVrVDk5OG/tSc3V HlRlCadXO+anXw2Qn1SZfCZWPrl5UHUL9+yoOxOeM+9JgMknbVtycZFNWvUUtImVLfLNy5b+1Atb iOCfuyQ90jdRqwAauFiwBodJ0VDqCK0Ok27eJbaOMBuSMgDdjT9whWESEYkcceRWD/GcZxbDc5+r f/ipn640o2hKtjGS+R/LFRtgyMwNSBvD5WYGpHUbhkAjz853Y+lJRTN3QXYkcb0OSRpXLuck1Wrl QBanRTGFWpvAosaknBxnNdWIJnAxnI966k+R8xH+t8z/2c== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0047_image142.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhMAELAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAj AQkAgAAAAP///wL/BIKGoetropz01JmuPkz3jWVcJILmOV6flK2oSrpvCs+gXH/Mbun2jzvJgjNe T/FLkoAvokfpfBabSEvVeqw+GlwHB0LreHVi8m58HkXFSLb6W4pxWWnzt2G8P/Lb5b7MlkB2BSEI NnbX0weXpsZn5UMY1wYZqJXVsnQUaZmVUufhZjTIk0d4qgipGlN6aRlJOapa2kppa5oqy/d4+zYH 64PTyju7iVoo2juIKbeie2xMd+k6bZqZDIjlLFis+IznZxuOyR2t3e2Men4Vy4wdzYmx+v25mjou S43bS23vct0PGAt83Qr+K6gMGrFv+RLp2QIIDbI/Eyd2WVaR4sMKPWDC2KHYYlkzQ4foGGLk5dMZ jOAkngzZciUrkjSz1axzUonOnTx7+sTSM8rPoUSLGj2KNKlSFUKXOn06oQAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0061.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Surface plasmon resonance
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0061_image143.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhGAFxAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgAW AWwAgwAAAAAAALvg4rvg493d3f8AAOJeX//MAOLy8+Tk5P///wECAwECAwECAwECAwECAwT/EMhJ q7046827/2CoKUpWjidAqtUqvnAsz3Rt31766jqV9rigcEgsGiUkl+q3XCZLTxZS6ZwmncCjdsvt Fk9QpLjJDJvH4jBrRfW63/D4Biyln9FQNno9HbflgIGCRnZ+fExriDx8E2BZg5CRkiBXfVVPeZhN WFaYUVGToaKjPm+IpKipk5VerKqvsLGys7S1tre4ubq7vL2+v8ByLmyZV8PByMkfinXNacqSj9A4 x4xqP3/T2snXzXR721qfmliuFAEB4R1K7OTD0kHp2oXPz0Dy8uq46CLZqfSJ7FUIIABAwXz6aqVD mKPFiEEAyzCSIK/gwYQKDQJgiMJOtXJ9/0BtMUaMJLmNGi1yxJgqnUWUHAp1U+OMJhd4HFxOKAiT pSqdEi7OSaPnUiNDOIOY+6DzpUqfLVNKXVlq4jcpNbFGdSpVAFWogpp27Ylh3CVHZ6tsHev0K9g4 Yrl6fTsBqMagOynShUQwL96gbsPFZasx8N4tfeV2NTwt8d2xeBkfJtIX70vLBiUjc6xYsea96D5r 4JwSQWfRR/fxfLy6dV7ULNERmA17oGu8CFjvrJ2UFOnOhGurCzC7OAHhG4ELME147g6FyptPnWyB uPHitX9LzQ2cNyFsNbRH90y97vXrsAc7Zd4dRu8Yi2ZU1v2XPmvkyqyfxx4i322N3Pn1mP93Mpjl hz8gqCedcvgFo99+/Hmg4HrjkUUJfPXQ814GYmHm2mWQZUbdgxDS1oF/UmGWm4d5CdUPfN9oKMOE Cy7YoC+ylWhcYDRSWKOLFz6XmowwoFjff0gK6NxhJJb4lZH2FRTgkQLCtqEPlbxTzAs9/mjjiDoe F9oFXfpY4ZIhXInHEGV6ySCYEAaQwJwJcCQeYQGeyVgBFqipVjxuBkoenOjRWWd1Z5aW6FMYFOAo n31GUpGSlFJp6YDlkSinoQxNeqlrU0bpF0ePOloWX4sKGlym2Bl66DmpWsSeoKQ+akJYIHLF4q4h 6mohXdZtmoCjCE2aa4rbtajbsWgCYOv/Q2Xx8Eg2CCanaqyr1uBnG3mA04I0ftYlbJ2mUoStrOeK KAGkkEJ7QQ/gnoqBp6J+Wmm9v3YxbUPu9kdnPnzSmySVoQ5MXwDlLnOqlqk586dt10bcXoGedAKe WseUZFOWjiwlIT8UFNBmZ7Nii/CL74Yk0CEOwyrxy3I1eIZM3hhS8x01AWRDAAakK+XLBhhwo0Ae Fd1yXTAn3dYMMxOdoc1lsLOyTTS4FLTEJQcaNJAFsjyRVd7aZfDY95Kdr8J3NG0N1EQ55DXVM951 tb0E4/ufAa/RYNImZBDTyTlKB770Dp4U7rclhmtCRTuFV93V1bFmXSHeXA2Ng9jI+qp5/+acM3uX 5b0MBgDky5a+Yq/IUq4b6I77LHhzrOdCo+pnSi4d7WPFXqTdZfdOd7262zLf3b4DyPvoeH8a/DpI u+68l8vPcudLuCuHwPXY2y4A6W9SA2+01j4vvnTRV2VDOzMmunWNeRqEvVPcf0nNMgqMbz/5So1k vgjTj7W+9STjTvzGcyM9dMwcBDmB2Y7HwAVyzQQm6VaWrKCVvlXQQB0rkgMnMLckFSwvCOhZ8VqD H/AQKXz1e939BhWTp60tg8woSs3exiWfiZBkyrnhtZDDjLVZKygr+FDphEhEZRWRV5Vjng+v8Y41 +RBxU+Pf5jzXGh2qyIgS0OEReyWcHv924zdBVKEYX2IYInWjEVc5YXzSxj+laVFRbHmjySgWwSgM Ly8l2ODv9Gi3MpYEcX4zUN+6xYlBFlJCIyxb8kClm/Xt8V7BSwGN8jjGMQYmXBjyVgxG9qMBZu1/ gYvdFbpEghVWsllYwqR7qtWfMUJOhO0D5fiC16ZShtGU4iufHDi5qKttbVayfF3soDSwSvDxmIkE DDCImUxRBQ1vAVLdI6d5tt0tChO4VCFjFjetLPyBW3HLZkGe2TPmVK+SQ0MRFdHIiikOEYtI7NwQ t+m2lNmznuFZZzzXSc6g5eac+3znrtIZmoIa9KAFTQJCF8rQhjr0oQhlHsOciA1pWUP/JEwR5+MM EMJTsuVGBwipSEdK0pKa9KQoTalKV7pSJRaFjWzEAxM1ySGI2vSmOM3pTWnA0p769KdA9alEX5ix lzYsRlfpQFCXytSmspSnTo2qVJk61JmUoodYQSpNMTDVrnr1qTP4qljHKtKY/BF9JPmbxcrBSguQ 9a1frdoA5krXutr1rgG4q14HkNe92rWvfqUrYAO7V12miUuEJexg/bpYxiZ2ro19rGDlKlm8Sjay esWsZSv712i0tTqc3axiL0va0Na1QZpNbGpPW1rVmnayuljtaF37WNlC9rWwnRFu+dra2dL2tYbV gm0d+1vfGte0qN3tcJfb29CW77Py/9ktb4sbWOYql7LAbS5xqcvZ6KmyP9K1rXW5213sIle7hUXv ZZlWx5N8xLznJW9m1bve6Er3vvhFbteQcqCsVnCTuDUoYwVcWAJn1sDltW9+F8zgwlKMvyyr6M6Q i9ADH9TCCBZshZ0L3wZ7eMEym6Ha/qtb5274ryc+bYo1fGEOK/jDMMYvDytasbTy7cWVXTGLC1rg DO8YHcDtcIyH7GLZKRfIihWvkq+LYyI7ub6xyMJwn0xlyAq5ylg+7SwQmOUuO7jJXs5yPhJH5jKb +cxmrkuY12xlCoKXykhmM1+37Iopy7nB8kiqhKrMYzYH9wZ/sHN4BR3jPM9UcSXQqf+iH7rmPxuB 0AEOzZ3z/DSdUYTPkvbzLRbN6YVqWsQBuQem7zzn2GI6042GsEfIRGox7wLSwI3zpxE9JFa3ms+v 5vOtk0tkVOcY1pBtrKOLAOxb6xfMDO4rpIs9XS2b2thwvnJ+Ja3svAo4zkC2drD5qu1sC/bbnX02 tJ3M60LfVtnT1fZtt11tbvPW2+oWdq7HTW5pT/vc+Pb2utP9bn6jO9PyFje9C21vGePb34MF7L/7 zXB17zu3+xh4rws+aB5j++Hcpra1La5xd/u61EaWOMGRLfL7DpsyJYdxuVMO4nmzPNkUf3mOXS5z GVet0zjP+aJpXvPwlkcbOg+60CEEKoEIAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0061_image144.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhpQBeAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABgCj AEYAgAAAAP///wL/hI+py+0Po5wyBIqz3rx7dSEhM37miaaOFV6sMbIW7L7qjedNC7g9/IsBg7qi Mcd7tWbCH/EIjXaSB97QSZVqtxVlrUbrMb/csvmMTqvX7Lb7DY/L54sS/c614/dHPf+PBChY5Ddo 6FF4qJiRuOgI0fgoSTJZWWGJuZK5CTI1wykXqekEGidaxxQmAyYjljp2WgqpkeXzhIVrGys7i1Eb ZJtLk8X7sTukC/SJjCtSfHK8CsZcBRt29YxoJHqcfWZD0u09Tl5uniB+Xpauvu015oqcKvL1Wd8+ pcxcOy9MxY6vij5h8cRc+TeMVEBaA39VO6jPisKFvqwZjAdr2kWFXqsoMvSIBxzIkSRLlluGzWQa gCqR+FjSqqWWGMkkyuwjkCDLmxSaOOTZp5U0lEC/FV0j8qjSpUybOn0KNarUqVSrWr0agSjWFDHx Df0KNqzYsWG3cu1q1kTStMZAFgAAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0061_image145.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhfACLAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALDoAPAAF AB4AgAAAAP8zAAIcBGKGGtv44lPp1EUTtvr6DmYiN24mI03T0YRcAQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0062.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Fluorescence lifetime imaging microscopy (FLIM)
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0062_image146.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhKwFfAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAgAd AVwAgAAAAP///wL/jIGpy8f/jIO02gsn3kjzD4biSEljQKKi6pEupi5xNUfvjacve+prUssJgbZL UDZMKhu7ZidmAkwOtShV5kBRWVmtFNrlPqUKa/D6fYrV33V6dp2Gx2B6udsQw/VqiVb+R4YH9reG FmHVQSao6La4eKgoyQNlx3jpBZlJKTm5OdkICqmJNcrpiWl5CofKQzr6WAX0+Qbr6kjDBpj5GCtK JMp7azJ1l/XbxtqYmNyreSjbXBzMZmu8d/zsau3sW02p7NdpBFsL3FtZ3nnrvT2LjqR+VBmtDGxf n9ceym1fFu8OHasz96iNUdethCl+wv4ZPMcuXUSG/UC5o2eQ1sRx/9ZOdcxIsWBFb/8Igkw1skSU ZCt3YdNFqE0fmTTnHEwjE8/NWcTq4PypkyXPgy3nbPETCOgZLklt0uRIJ4w4pN+YLrmKNavWrVjT JeQKNqzYsWSvSiyLNq3atWyxJG0LN67cuXTr2r2LN++QQj2LTkXKS6/gwYSZ/PKqEWrhxYznmiQZ 71zjyZTXbkPM8XHlzZy5ipvJM5rDzqRLJ7k8LqBm06xbhzDpb7Rk17RrK4zsFbJi27xpr3wKGJDs 3sSL4zhiPDll5E6UO38OPbr06dSrW7/O+SjzstvfWe6Oa0l3YzvGN1fJwSM5IeYNzwPR/raFwGCR B/zBNj4nesKCv/8hxNdRnvSnizZ1TDXULtIMJVRwgWknTU8RSshMTltEdeFnm/AVy4HEXONMNnKg IogXJja0EGqMrPLMJfdsWMUniMkYCi0LrfggjSjpyIeLLLKYyoVBdoPGg+bA45GJKy5jIzgopciQ Tm99pIGSwEXp3Ye+FPmklTxStF+LFoE5JiarCFljNSEhuQxoHx1GhIgFfZklRGNW+eSdsp0VJ0ao fbkRjTaWSSePQNo56JoDrVNOIf9JmNM6L0FKYV/YZPiZUEoFCCFOmQ5DlUtWmQHTX3/1AaGAngpo alRuBRWqh035VF9jtMaH327DiYXrc78RZkav8DmFiLDHtYBdssr/Lstss8UhW156MWaqqxJLvYYr tPrYtZ2xe+UC32tITNmWsfp9VQR3rjrqUkkNmarqf0deieqpAJ4YKoMAvSJVhHUS5ZNwSkm6x5EI EpykwQH22eG8FllpcEm1eKnoHREveVgg7+6Y54s3zlkmxkSG2EqcjbbJn8hOKhlHmixTHMmNgkqm Jbve+Wiyio5SIyM4GnHp3sMOJRZTUG2CPGuTFfsMcomA4vg0yUOqPFrCIyN6sos8n4yizkWwwyjH uUGGGdV8ymyy1mifFWbXaLeiHbs7N7jwp6XQfXO7dLPq36rQxEpVgrDq4UjgeX/KlKx+a1w0ppWy FLe8kKKFUXTe/052OWmTO/drbZ07C3rooo/embbhoheX6dIap/rNkd3Quuv25WpY7dXmkLntp4eV O7q6zyfu7mY+VaLkGuqLqqayQDMh36dqulNVCk+4U4YWA8zkzqkiLpK/8hjioMIf+hfTOz26zCbG +DC4cctEMwo2xD+tDfPQqWlsMWaFsvm+2yKrfRn8+YlKqUEf+wo4KpRFxCoFclrD+MExs9nvRxDk hvpyBhEubcSATOPT3IonEph1cEDyMCD80ne2tDUFNxC8z6IIWEEKTu0hFEtT2N5mpBqi6E1VC2GX cJa1xkEuNMZ7HvcstLlY7W1GpIJc+WbCt0cJ7lVvoRThRqU9wP/4bVX1qiK1Agc4uWlxcbapnK9I pzk0KedzaGyjG98Ixzheb3YZ6Ja5elesMTqIjdczwh0/EJ50CfIEeGTPIOWzL9p9K4ITg0EGfrcB YRUSd5Rc1zWEE7m+YdFmnJLXgi7phmyMa2uzsV6pLETDxDGMKJd8URMHQTiBiM+QK6taYq40M52x jEyRCtv56FMTgRWweIdS3P/6sbG0DclqYMpRI30Xrh/NLEUD7KX/6keKAOrpiSrc0p5CYrVdotKH EPtNf8IEwmIUc2qTpJOeqIlBkoTDhjpCSC5nQzM4wYOR86yhnaA0NiDS85/uxGY7GZe0UsHrYMkT iKU2RT7wCdH/U1XhVLykCEby5MuTFR2n3h61R4AhFKIInSRc8BjQXcmlnXL0TCH5SLyVvjR2La2p TW+KU8aMx6QroJVKckfTSG4lqEDlKVlWs0ihIuNrsNMpr/KCKbxZ0WFj7FD48scqskURpFz1EYcA JFJSMRRDx0BQ3IIFo1hyky7ibGEy8efLCz7wY3wwEjvfJEBe2kI95nDbNLPJSmJeJK/QtAwy/lrQ Bd7Sr+TE010DtTY1oTOHYoJSL5dkiGZK7FB3kR9i39ZND7ZwtB9r2v5kmKjJ8i8zAz3hhghFEHfW 5XFaDOlWnSKVYJkvtxytbaUkikpVCfOqDa1SbgA3RLQuLDQl/72OUZ/qgudGF58sbCNM8XJdGgR1 qBctVk6/C97wiheQPq3j7VaXle0qFVz1mdJ92LNTopqle+x9nfBUerrMSddaw9QKHd/7SGtldWxW pO2/JOtEuBIzQfK0lHAbOLDF+SxemjybKqd1oi0Zblq4wZdjT+OxcobMQzhjm9gwezH3IY2X42PC 8lqrP37dUMFt22XA8Fuwtj5XtC0RmmXPOScN4suajTXxmTLrAfe2yIxllWuMZUnkmtkzyRMUaFI3 SMMf64aZK4wrKVecJ1Gur2L4tLEFK3fcpoG2uitOaXkSxzhQ6laJRhuRlMOatKie0ihfNSe1HDoN sjr4eHhrhiBUNWS4ACv0ePulTqMVeZ50Pfq+4M0u70wa30lTGgcFAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0062_image147.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhRAANAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAQA/ AAgAgAAAAP///wJQTAB2yJ0Oo3RrolotzJp2unDfM3rlc2AGuCprkqYw47ZxGF+oztOzTMr5QqQZ r3Uk9nREHPLy7L2CR1+RA8w2lEAppqrwYl2wV815+9WgsQIAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0062_image148.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhMwANAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAv AAgAgAAAAP///wI/jIGpy63hnoOx0irbxWxzehgiEkLmJY7lCRxtEr4yC89yi+I2yYMqHKuRciCe 0WQ89jK65Uu18qWGK12qaigAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0062_image149.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhmgCaAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAACW AJYAggAAAAAAALvg48z/M/8AAP///wECAwECAwP/CLrc/jDKSau9uJbMu/9gGG1iaZ4oR6Zs65br K880FNd43t5673+8n3A4CRKPSIAxyfQtm9DakziNoqpCrBW2VXZpWud3Fu6Vx6ruGY1Z49xsjTq+ S1WxcLokv3ec+XoPf0obBYWHhoeEioSNMYCBDWWFXpQLlo6XXpWYkJGaF4kKJI+blCuWqaCfIpOa mJWjsbKkpqusIH+iho6JvL7AvYyerMQsxpHIV7itc8y5zs8eyifUdNZc0tPR2hnYzd1pW99o5NDh 3tzocuPrberufVDA5u1RvvHs86L5ReP18JLw6uePYDGDBxEmU7iQoR6ADk1AjAiOYrkuASzeahIg /6PGTVE6fgQJJcAAjxYnOkDZwORJCCwxdpw5U5+Pmi0HvGwp8ktHAgCACg0aUxARkz0V0ETZcecW kUAVRA2qtCgDlSt1nmTas6lOq0kCDB1LdShYkj9cZpypVaRan1SlLpga9SzWrGu16t2KFGPZv2SF gr27EoDLvW0N+yUrd2hjq4SzIt57FolYwJjJQsYwQEJnD4cnJ346t7Tc00SNCqkZWvTLpGEzyzar mkHnvQp05v6sWzeA3rd5qm3tOi/OIx7pmo7LvOiN4NBz/17wWTrv38Gr6vXqOvFh2KtniyfgvIHW 6dlv866efrr7BWy5d399HLly1PeplrdNHb30///utXfdA27N95ViIQU2HoKSOGCgbxACh516EhCH WGXIYdYYc4LVhtx8GB6l4Ij6eRiefJQZFmJ4HC431WAfekRcgSumteBYMB51oIo0KTWaFSjlR5dd Ou4I01uLjVdZZFXBthQDTdWYVnLLgefHEFYaZiSUUq6WEY4rRrbUmF+N2SMTZqap5pVpCeDmm3Ai BeecXc4QwJx45hknmzfp6WZHfgpQpwx3Bmrofn36CaiiHBl6KJ89FOqonoO+IOmkeCIaKaaUNspp ppDqcOmnf3pK6puainrqnmiuimqoOYxKaqUuyPppqrG6WmqruuKKg62c0toCsJj6WgOxkwrLArL/ jhpLA7OP8uqqs3bqKqipq1JLqLXKpgBtoNpayi22p4Zb67jSZgvrr+iG1e5GufZK7qzrHvsucvei Fe+0895a77P5YpmvmAGvNrDB8qZb7r/VJuyuw/CyCzG+E+srMb8K09tgmxULXDHBHSOM8VUiq5ux vxsnOrJlB3O8MsUvW2xvyC6bTHLNC58cLMPb0qyyzRHPHLPHMYM8dMk53/xz0ix/jLTGDxf9NMpR Ay0LzlA3LTXWVGtttcwA+7yp01zvrHOxPIsrtqpkT6nm21liCTfcaY80BJN2F5T3PXtbgXffJgJ+ xN+CK1344IcLlDgShCfe+OGPFx654JMDXnnfKJfvnXnem9vd+UiffxS6RqOntDjip99Nz+qst+76 67DHLvvstNcOewIAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0062_image150.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhmgCaAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAACW AJYAggAAAAAAAADM/7vg48z/M/9mAP///wECAwP/aLDc/jDKSau9OFejtP9gKI4QR55oqnrm6r7w 2cZ0bUfzre9vzv/AkC9ILFKGxqQSgFw6gc2n9BadWmHVqzaV3XpF3a9YE4aOe51p+sxdP91sUvkH j4PrSrz9M+fp92R/RYKAF307hIUbiRiEiYyKEocWcJALlpEPfQomnZwcnkyhoqRrmJkNc5xMrKas l6uXr5+vtaiBjS0drrutDLHAv7d8kGmrvLKxvsvGwyyWoLSe0aLRoKWjp8OTVM64Vtq33DbhqOM1 5ZnnNOmR6zHtiu9Y3hnzPfW5V/GF9y78gPytALhHoAqCdgxyyWcIoQxqDCcolHMtokSHDzGOmUjR /+IRjXI8XhSZECRJLCZP/kup8iDLlig4whz4cuYImTZj1nwS4AvOKwF6bvk5JUABoVqIegjKdIXR o0N3VmgKgMCCoEuRNniqlQHWJEotBLBKtqrZrxeMogXwFOrWtYOkRgha1uqCul0pcEXKlC/XJWEl 9KxrljCBvIILKBbal7Fit3nkPhjLwK7Zu5URz3XbdnHPv4AlbzVcuLRVzRCwBn3MGqpaJ4EnW55d uXZj1A46t3b9RvRV08DLBhBAvDjcybt344bimy3m55fPFp9+e63u5JA1FJiwnRiRwcFLD59Onnxf tthbf6YaNwh4zGUxjy9Pn3h1pulXexa6vfUCxf//dQcggAAM2N9H7pFG2Hz1NWgffukd1VZTB1b4 X4EMdHehgAUeKElzQtEmX3lBmcegAH1FyFpjDTyGoYf9CaghjBjWiAOI4Z1mXF87foYie9clh9qM L15oZI00cvjhdyKedd5p51113FUqZvdAlQRmaWCHMS7pXnh0mWUNRGNaE+SKajTnXJO0KUBAMLV0 EoAC+inH1nJ+qLlmjlUZ8GZPvSxzyWt3rvjZYrDpGWJp8FUDizE5GPCYX19NmGgSWCnY55zJCGOK Z14BCRpYenoV3WycCjOLMHNaN2luUzLnRF9kMVXRNWV+Aseob0Xm3n3A3jZBsHvd5ysQAQyg7LL/ zDbr7AC4JfsstEdNC+2xP0hr7bbXCrZtUNbiSY5v2nI7bbTfltusuOiQa+63w757LrY8qCvvsuje uy69O9irb7764suvDv7eC3DA3RoR21YIM3twwOyy427Dyj7878A3FCyvxQZjbIPG73K8scc1gGyu yCGTTIPJ3KJ8ssoxsAyvtxRHDM/ENcebM6nuUVyxzg3bjFLPPrvcMswwyBwu0AgLjQbRO89VNNIv KD0vzUFT7YLVzxo9s8I4Z41101o75XPCqU3NM7Jne7302tm2zTTEZavAtbNuXw021GJLHXV7bKvt d9+Axy142n8TsbBXco9NN9z1Nj442ZD3KzninoQrHjblkz++d+CJ53a45nxzjrnppIOeueihy6o6 6qyv7rrhrTNee56le3667qnTLrvtv+P+Ou+xwz575KMXT/zxlifP8O2IkEvs9MNOH2zdOdFRavZe cp+m9+BsD74Di4+PoPmXoh+a+uuzX7n7zMOvvfyF0z+//fHjP67+9/OvQ/n4A6D9BEg/AsovVwhM oAIXyMAGOvCBEIxgrhIAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
Protein affinity chromatography
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image151.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhVAAhAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAgBO AB8AgAAAAP///wL/BIIXau2dnpx0mQjt1OdZTnXhGH4S6F1IZGZqtqwyo8mxHYvqx7PXr6sBc53c Lfgz8ZAilrAJZCqTRWiSiI1Ol8Outrut6orC1tP4UuBmaedtvWJ44fI6nYTP6/f8vv8PGCg4SFho ePiHAojhguigiKgISTJT+TaUAOJEYwOBOUlJBTWlxjQqutXiR8q6acpq9abaByvG4fNlG7ubmOka 5+vbiLG2w8m4B+rouWzcXIH8LD1NXd0cnYe9oVyx6K3HfeKqVlNOHnflxkw8jme2HhuGdku1RJrs dXo6X0pPxk+Gzzswc+qZ0ldFVThjjNDJAXYuWENz/ToV4rbQmjttBWk0TigAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image152.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhTQAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQBH ABQAgAAAAP///wKuBIKmy+1/XoI0GEtzw1rxyiAWlojlKB7pSV5riKKq2kZRi3uXnd97uZiMgkNf bwjc+Xg8IVOHzJGSRdzn+FNmbcqrsWuSdYkxl0sWntGanvSkE4LLdfP62a554/f8vv8PmKE3N1jx 8VeDdygYuBZ1MsOS1GZmFga5KGH5VGXFxVHjhBTZEcrJ5PRJN5U1ygWCOsa6SscptZS5YUIzy3up piZ0OSzWWJtrnHynDFEAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image153.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhWAAWAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAIAAgBQ ABIAgAAAAP///wK6hA8RyKwN4YvUVdkWvlTz9EWespCTl0zpSoJGmz5mfL4tipSvq4PjrundeIcg ceUD9hw1Y1DlBNqkLqIRCRNuqNoclxethp+74jFTkpnUuZH7lj6pyXBaOoTPi/T8vr+N8yc4SFho eKinwhEowsinGOJokSfZVymxpriGdfdWt8k0ZRM3I2PmViZmtdSls6RqJeWqNWb2dZUU+/oTm+Ra Rpv6U/vlWwVb++s0EzOVycIGOscsKkeaqVYAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image154.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhRwB0AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALEMAAAAB AHEAgAAAAP///wIJjI+py+0Po2QFADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image155.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhRwB0AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABE AHEAggAAAAAAAGb/M8z/M/8AAP+ZAP///wECAwP/aLDc/jDKSY2iOOttt/9gB45kJJZQoKLVxTYB QQSvdNZAPOPQjas0UCHj+wCDrIISU/QEBAIkajhsoZ5RHtHlWFGA2m1KJg2jirqdY2n2NGNehpLd Fqcg1Sp4Muc1NwEDA2VrVTh/GYGChA10NYiJe3UPkJMslZYlmJkjm5wfnp8cXKIvoaVMpKiaqqud ra6gsLGjn32Ps1qOl7k8u2e9tFbCJCe/xDYXc4bIE8bNGqfQANLQ1c3XgIwjYNmJZC9YNN4YOttO UOPBJUc13a2S0wxvi/IN9IMeyzW3Dm/xGI5NYbaA3AOCLxBSW2dvYUM7Gs6h8qQiHzKKgiwKw6hx 6yOXOA8AgmwD8oQ4QOkmmZvn4mQiKFnqrCzYEuCEJyO1qFDj8MonKZ4Eutq0DxvDBQqHHpVnMFZT paIkyvo5SGq0pSQUdQQgNBnVrVyTev3JqGsPrGHMnn2Yiu0wmVZDoAUETsvTCDP9zI1ok5dbZ3v1 iQ3o6C4ftRGK9rQ0mLC/wBsQ1/prAjItw6Uwi9L8iTMnzwva2cXa72ZdvSPU5j2EFrFo1Oz6TiKn KC7srPUyw8qZwvZtGCkfooHpeyK84A3/8LZm2WlzqJTXxrYqW+6V3BJc+mWHHS9yYFeWwxA/NTql 56tAW1I/OwEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image156.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhRwB0AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABE AHEAgAAAAP///wL8DI6py+0Pg4G02iuv3jzzDzZeSIZjiWpnykZTCztrTB9zHd94q+9p7ysBg6YX kWc8/pJKIbNZhLKGUgu1SrliH9qt6Om1gsPZMZlrPn/Vmy4b4GbH1fNznXwP5737bR/7VxUoNQhV 2HSolHi0SNQY9OgTuTOJU1lzSZOZk/aWsAkDiuQpRlohOtVpirpkWubqAosmK6NKyoqC60S7xrug SwIc5asgDGL8gdxh66ncxvzmrAItR01nbYeNp63HzeftBw4oLkhOaG6IjqiuyM7o7ggPKS9JT2lv iY+pr8nPSVzMXyiBowAikIaBYCqDBxW2YgjHYS6JuyAitFIAADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image157.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBQAEAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAE AAIAgAAAAP+ZAAIDjGFWADt= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image158.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBQAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAD AAMAgAAAAP+ZAAIETGAGUAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image159.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhDgAHAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAK AAMAgAAAAP+ZAAIIjIFge8iZgioAOy== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image160.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBAAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAD AAMAgAAAAP+ZAAIERB5gUAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image161.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBAAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAE AAMAgAAAAP+ZAAIFRGKHCAUAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image162.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBQAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAD AAMAgAAAAP+ZAAIDjA1YADt= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image163.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBQAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAD AAMAgAAAAP+ZAAIDjHFYADt= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image164.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhUQALAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQBO AAkAgAAAAP///wJfhI+ZccGqnoO0Novtoxv0vIDYFEkkM6HOunppy7quZMQfFIc0KvPeT6sJg75i b8ZKthBG2PAJvER/06VRQ9JBqU2psArtXCu32uuMTn7NKhO7ZyuL5pwQ/Y7HZ2d5SAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image165.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBQAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAD AAMAgAAAAP+ZAAIEjA8QBQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image166.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhDgAOAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAK AAoAgAAAAP+ZAAIPRAKpdqvtopyUQnnXQbEVADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image167.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBgAEAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAE AAMAgAAAAP+ZAAIFRGJ3YAUAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image168.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBQAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAD AAMAgAAAAP+ZAAIEjA0BUAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image169.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBAAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQAD AAMAgAAAAP+ZAAIERH4GBQA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image170.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhEgASAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAAAN AA0AgAAAAP+ZAAIVhB95ixm8Hpy0GlWdfdL6/2FZRyUFADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image171.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBAAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAQAC AAMAgAAAAP+ZAAIDTGBRADt= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0039_image172.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhBQAFAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAEAAAAD AAQAgAAAAP+ZAAIETGCXVwA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0024.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> <= /td> <= /td>
Background:
• In yeast, galactose metabolism is controlled= by GAL4 and GAL80
protein
+ galactose
- galactose
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0024_image173.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhywAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAC/ ABEAgwAAAHBub4KBgp+fn5KRkqurq7a2ttLS0sDAwNva2srJyfj4+Orq6uLi4vHx8f///wT/EMhJ q704682BOEsnfaHIMBYprpTKSif2PM2EzPSr7+Iyr77HKlYJ8jpGHdHyOFQGDcKREhBeqtPNb7Wd drMyK3jiWAgoqDGgIK6w1ZcvR76jwwH2KSEnSQzUAT5XgncUeWFgh2OKPAoPBmtObj4NZwABCTMM fx4ODzcADDhtoaN7M1E4Tg0zUgOiDpyIBw8OAjeoEqwPpwKeChMCCgsBDiguAASebKQSmJp/KrQL Us6ZD5sTM7h8uwS+n1bI4KAAC9RpFAMPAgK1Ep4CeyW2HlZ5XYFiDgkTxMmPALCSxSTgOgcADGCj 4CAAngd/MiGQEKRAAYRJBCzop7AZgHjz/8zNgLQHITx2ISX8UJjuo8N67ih2gWllHS8LokbBMLnl QT9DHh9ScDTxocMCkjJJWNeyQpctrApIKFB0izuTRoFKoGXJDgOe98R0+apSrJWoU6v+1KpyrTaP eQrkPMkAWFlEE6ogNACixgGpQu8+fGpWQkkYbxMLVkwo8AW5PQtTgCz5cCgy2Owu9lSXrVN2FXwx ODXhml18pDLJDUCLQAnHkQkrFviJgCTYr+VEnt3CGGnYYkT/dswKgW0KpnknX4zG5yV6YSnYjh6H VMljDzZOEMXJOV7H8xI4FOzutm4rnqS48+hJsOyTi7uEHz9hOu9ktEbMsDR5lKT9650BWq4xMglQ QxHsHHgSYGgZtpBC1VTHmygKiiRVAv8wFxhLANiE1Vvt7AcciAEyR6EhZxAYX4oIjUIHAj6sxYYt X0nxwEX1rIENfxOwwQCPb0xFygCcEfTZDzoJGeEltDRBHw5YJQmjGQscwKOQNDpwCpI4YClAjYOJ wcaSNzqQY5Jl5ljImmv6wuabGrgJ55x0boAAYHXSeWeefObpw2t9rvlnoIS+yQA1ha556JI8RAAA Ow== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0024_image174.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhygAhAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0024_image175.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhygBBAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0025.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
<= /td> <= /td> <= /td> =
Background:
• GAL4 consists of DNA binding domain (BD) and activation domain
(AD)
• Activation of gene expression requires both = domains in proximity
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0025_image176.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhywAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAC/ ABEAgwAAAHBub4KBgp+fn5KRkqurq7a2ttLS0sDAwNva2srJyfj4+Orq6uLi4vHx8f///wT/EMhJ q704682BOEsnfaHIMBYprpTKSif2PM2EzPSr7+Iyr77HKlYJ8jpGHdHyOFQGDcKREhBeqtPNb7Wd drMyK3jiWAgoqDGgIK6w1ZcvR76jwwH2KSEnSQzUAT5XgncUeWFgh2OKPAoPBmtObj4NZwABCTMM fx4ODzcADDhtoaN7M1E4Tg0zUgOiDpyIBw8OAjeoEqwPpwKeChMCCgsBDiguAASebKQSmJp/KrQL Us6ZD5sTM7h8uwS+n1bI4KAAC9RpFAMPAgK1Ep4CeyW2HlZ5XYFiDgkTxMmPALCSxSTgOgcADGCj 4CAAngd/MiGQEKRAAYRJBCzop7AZgHjz/8zNgLQHITx2ISX8UJjuo8N67ih2gWllHS8LokbBMLnl QT9DHh9ScDTxocMCkjJJWNeyQpctrApIKFB0izuTRoFKoGXJDgOe98R0+apSrJWoU6v+1KpyrTaP eQrkPMkAWFlEE6ogNACixgGpQu8+fGpWQkkYbxMLVkwo8AW5PQtTgCz5cCgy2Owu9lSXrVN2FXwx ODXhml18pDLJDUCLQAnHkQkrFviJgCTYr+VEnt3CGGnYYkT/dswKgW0KpnknX4zG5yV6YSnYjh6H VMljDzZOEMXJOV7H8xI4FOzutm4rnqS48+hJsOyTi7uEHz9hOu9ktEbMsDR5lKT9650BWq4xMglQ QxHsHHgSYGgZtpBC1VTHmygKiiRVAv8wFxhLANiE1Vvt7AcciAEyR6EhZxAYX4oIjUIHAj6sxYYt X0nxwEX1rIENfxOwwQCPb0xFygCcEfTZDzoJGeEltDRBHw5YJQmjGQscwKOQNDpwCpI4YClAjYOJ wcaSNzqQY5Jl5ljImmv6wuabGrgJ55x0boAAYHXSeWeefObpw2t9rvlnoIS+yQA1ha556JI8RAAA Ow== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0025_image177.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh4ABBAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAADc AD0AhwAAAAAAAAAAMwAAZgAAmQAAzAAA/wAzAAAzMwAzZgAzmQAzzAAz/wBmAABmMwBmZgBmmQBm zABm/wCZAACZMwCZZgCZmQCZzACZ/wDMAADMMwDMZgDMmQDMzADM/wD/AAD/MwD/ZgD/mQD/zAD/ /zMAADMAMzMAZjMAmTMAzDMA/zMzADMzMzMzZjMzmTMzzDMz/zNmADNmMzNmZjNmmTNmzDNm/zOZ ADOZMzOZZjOZmTOZzDOZ/zPMADPMMzPMZjPMmTPMzDPM/zP/ADP/MzP/ZjP/mTP/zDP//2YAAGYA M2YAZmYAmWYAzGYA/2YzAGYzM2YzZmYzmWYzzGYz/2ZmAGZmM2ZmZmZmmWZmzGZm/2aZAGaZM2aZ ZmaZmWaZzGaZ/2bMAGbMM2bMZmbMmWbMzGbM/2b/AGb/M2b/Zmb/mWb/zGb//5kAAJkAM5kAZpkA mZkAzJkA/5kzAJkzM5kzZpkzmZkzzJkz/5lmAJlmM5lmZplmmZlmzJlm/5mZAJmZM5mZZpmZmZmZ zJmZ/5nMAJnMM5nMZpnMmZnMzJnM/5n/AJn/M5n/Zpn/mZn/zJn//8wAAMwAM8wAZswAmcwAzMwA /8wzAMwzM8wzZswzmcwzzMwz/8xmAMxmM8xmZsxmmcxmzMxm/8yZAMyZM8yZZsyZmcyZzMyZ/8zM AMzMM8zMZszMmczMzMzM/8z/AMz/M8z/Zsz/mcz/zMz///8AAP8AM/8AZv8Amf8AzP8A//8zAP8z M/8zZv8zmf8zzP8z//9mAP9mM/9mZv9mmf9mzP9m//+ZAP+ZM/+ZZv+Zmf+ZzP+Z///MAP/MM//M Zv/Mmf/MzP/M////AP//M///Zv//mf//zP///wECAwECAwECAwECAwECAwECAwECAwECAwECAwEC AwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwEC AwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwj/AAEIHEiwoMGDCBMqXJgjh8AcOB46XEix osWLGDNq3Mixo8eEDUOKHPmxpMmTKFOq/JiDTEiXP1qGjDlxpc2bOHPqNDiyIc0cP0PuHEq0qFGG PmW6XCozJs2jUKNKVdkwgFWrY35oJfMjgNYAJKeKHUtWoUyrZKz+4MpVTYC0YGO6rFm2rl2oIr2u XXuVTNqub3veHUxYZ1KwcK96dctCLdOYhSNLNikSMGC3VtVgRvtjTNjJoENXfJlDsdfGb92q6bpW plDRsClPpJuz8loyanBr3r3br1atr2MLxwixZ8OhQJX+WM1c83Lnq5nOHU59tPHPNkduHcO7u2bf XH9W/x+P8CfMpjINN1y6/HLiq9HRjyFP/6HpHJ61jgFb+vhNkfnhlplmARD4VnjA+VffeKWVkENj bLXUVQ4mBOAZTiF5tlQAjZkgYG6qtebShQsy2N9WgHmVFlwiZTcSV4oRiNtf4bVYInUhBWZagYA1 piFtKK2H34Nv/dBYiGgpFdyNseX4FlrwbcbfkkGOtOOOnWHHpGgyrGcaYwX+5RZTQJ40Q0M/sqem kpBtKZxtfoHo3V5B/VdZcvoBF1SZbk4mk4btNQdie2TyKRt6I/7pmqF9FjYTUNzp5t2gvyno4nXG NfpmchvGaJoJyynJ6EnaCabppkDtlRZmBBpIxhg1Wv9am6mnosreajGyhiuhyY1a66/EzXTbmFit yld0rckK7LIaJQforiXwuJha8c3lK7PY8iQsjIql2BedNmYrLkWkSaekuUz9OO66Zj2a1Lu9xlsn u/QWhOeQMeV3r776WlvvvxJhKvB1ABds8MEIJ6zwwgw37PDDEEcs8cQUV2zxxRhnrPHGHHfs8ccg hyyyZAOXbPLJKOfgwsost+zyyzDHLPPMNNds88045zwzQtaa5+6iPsPbM2lC/zy0kDS4kIULSS/d NNNKQ+101E9XTfXVU2ct9dZWa90111iD7XXYX5dN9tkIpaz22igzTQMWbsNNw9tx1z233HTfbXfe fOP/7ffef+steN+AFz544IQfbnjiPJ/ruEuKOYju45RPbrlMS2dBQ+abK9255pyH7rnooHuOmlWj W/V5AKR/7vrSVrWe+VWl1/766LjbLnvut5denn6p/pal8F6pJfzwx+fHGvDHIy+88r9l8QUWWVRv /fTWX0999tJvnz321l+FRezhs1597OB/z331X5Af/vruq7/+F+tLXz/9899/P8+qSsiWiHxhkVVO 954nmcZbaYEQAP+nph/QbxD2g+ADI0jBCVowCxLEYARjJ0EOBaB9LJCeB+mHmiyczirto93pThi7 Eg7iKhAcYBZkKMMLZvCGFdSgDXPIM7aA54c+VAxc/xJTwBR1xS/d4oxffMjE25DhC1AcBBS/IMUo TrGKVLyiFq3IxfZN8UpUVAwUUQjGK5nRNGMk4xmtgsU2bjGLXXRjHKGIEOYE6o52DNGuwKSaAvkx M9LyoB7xSMjVKOILh0wkIhepyEYy8pGOTCSHEGmaxhzyKou0ZAAU4UGrcHKAAYhWhzzIycaYkpKe 3ORVTvdJSLoykq+M5SF51ptafmc38PkQEf24KvhcaUy2lJMwcaMIRRCimMZE5jGLucxkMlOZ0Hwm M9foyU8iM5XYLCY1FfPJqxDCk9+0SjhN08xyRtOZ6DSnNNFZx+Xgxp3Meeeg5inPesbznvDMJz3x OXUoWPpTlgD9p0AfOcCAAhQ1Bh2oQhP6hTpO6qEQjahEJ/pQZFr0ohjNqEY3ytGOdtOjHb0KSEdK 0o46VFDPQeluUsrSlboUOjBVaUxbqoZEFhORN0UmTm3K0536VKdAzalQexpUog71p0ctKlKNytSl OlWpAQEAOw== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0025_image178.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh2AAxAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAQDU AC0AhwAAAAAAAAAAMwAAZgAAmQAAzAAA/wAzAAAzMwAzZgAzmQAzzAAz/wBmAABmMwBmZgBmmQBm zABm/wCZAACZMwCZZgCZmQCZzACZ/wDMAADMMwDMZgDMmQDMzADM/wD/AAD/MwD/ZgD/mQD/zAD/ /zMAADMAMzMAZjMAmTMAzDMA/zMzADMzMzMzZjMzmTMzzDMz/zNmADNmMzNmZjNmmTNmzDNm/zOZ ADOZMzOZZjOZmTOZzDOZ/zPMADPMMzPMZjPMmTPMzDPM/zP/ADP/MzP/ZjP/mTP/zDP//2YAAGYA M2YAZmYAmWYAzGYA/2YzAGYzM2YzZmYzmWYzzGYz/2ZmAGZmM2ZmZmZmmWZmzGZm/2aZAGaZM2aZ ZmaZmWaZzGaZ/2bMAGbMM2bMZmbMmWbMzGbM/2b/AGb/M2b/Zmb/mWb/zGb//5kAAJkAM5kAZpkA mZkAzJkA/5kzAJkzM5kzZpkzmZkzzJkz/5lmAJlmM5lmZplmmZlmzJlm/5mZAJmZM5mZZpmZmZmZ zJmZ/5nMAJnMM5nMZpnMmZnMzJnM/5n/AJn/M5n/Zpn/mZn/zJn//8wAAMwAM8wAZswAmcwAzMwA /8wzAMwzM8wzZswzmcwzzMwz/8xmAMxmM8xmZsxmmcxmzMxm/8yZAMyZM8yZZsyZmcyZzMyZ/8zM AMzMM8zMZszMmczMzMzM/8z/AMz/M8z/Zsz/mcz/zMz///8AAP8AM/8AZv8Amf8AzP8A//8zAP8z M/8zZv8zmf8zzP8z//9mAP9mM/9mZv9mmf9mzP9m//+ZAP+ZM/+ZZv+Zmf+ZzP+Z///MAP/MM//M Zv/Mmf/MzP/M////AP//M///Zv//mf//zP///wECAwECAwECAwECAwECAwECAwECAwECAwECAwEC AwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwEC AwECAwECAwECAwECAwECAwECAwECAwECAwECAwECAwj/AAEIHEiwoMGDCA3mWMiwYY6EECNKnEix osWLGDNq3FjQoUeGHEOKHEmypMmNOcgwVPkjZY6WKhf+OEmzps2bOAl+bMnz48OcQIMKHdoxR4Cj LAKoXGo0pdKXC4lKnUo1JMOjP7CO+UGGq9KjHquKHUu2Y8ynXdUcDaAm64+kTEGWnUtXaMMAXtce JaN27Y+eUesKHmyyIZkAJd6yDZC0rV6mMQlLnqzRod6vR9WoVXOY69+GlENTXhhTrkDSLtP+aKuZ 9WrPLKGKnk3Xp8PTMl+OUbO7te/WsEHTHl41B47cY1wCBpB76Wvg0F/H/Um8utDLUHkGWLi9IUyu fH+3/+ZLpmtL09bT3wSbNDnT7XtXpl7NmL5eFsHdU1fPvyT3lVlhddhh3THEk2eZacZWX239dV5g /UUoEgVGqXQZWwHCld18SZWAoVKOcZUahBKWWJlReO21Fl967fTDGI9tVp5SL95m4o0YOaWXWo25 pVZsPRFon1sEjkgijkhGZKBunInXVlel3XXhZcnZmOSVCaGmGmuarcZXcA/qB5mITJ2H5ZkHecdV b05+ad5OGwImHJp0AvCgc/f1tSOZDFWp3J9R1ikocwDOyOBmfZX3WWC2WTkonTm455meJbDYJIZl 4jBQo48OuuRWMxLIGYiHjfEmep2mWhRMKdHXl6sBPv/p0pGqquoQbJcR+NiptNbaKYXzQTbimGXu 56utufWkbJwbonpsp7FFC5W0rLLa67N1NqqtbdimGlZFPnWL5Lbklmvuti6kq+667Lbr7rvwxivv vPTWa++98Zamr3yo9Tvrv/v6GzDA/LqEhQtZuHBwwgsrjLDDDD/c8MQSVxzxxRBnTDHGG2tssccc f9zxyCKXDJVHD3oHJ8or39qydzTEnEXMNMwsM80214zzzjf3XPNlNx+VcwA86+yzzUcVPXTSOTet 9NNHQ2301DYLa7VLepUw7NZXd8311l/oHPbYYtNA9sxkh4222Wyv/cVaWSQ9ttw0JKU222fjzfZR aQf/4Hbdfut9d9pl503434PrjTgNL3r211agOrhVing1HvnjjZvn1uWWO9655pBn8YXopI9ueumo n6566qTz3brQrsP9Nt8Xxr3W7FPSHrvQuPd+O+urBw/88F88Kd3xxv+IFVJr6aniZQGqCBfyk1Y/ yBeEiI59Ftdn/8X23Wufffjfj799+QF0v1aHAWTP/PW6I8ZYAG8nxfz9l9mP/VqErO8/UuLj3vnA N0DzeY+ABzSfmxb4pAXu6HmY8YsEWSRBTDEQPA1Uwxe69z3sbdCD5fsgITo4Qg6G0ISzg9+F9neU EtIOd/HLXQyPokL62XCFLSShCHWIwhKC0IM93KBr/4b4nOdgKEQ8WsxmFpOZD81vM0UkIhEV8QUq WrGKWLyiFrPIxS1acX65K0EV+UbFoyhiLWekXxrByEb7BWCNSTkjC8ZoRjaasYt49GIe90hFJ/lR T61ZUPOUyMRCPvCPf1SEIhfJyEY68pGQjKQkI4nGSU6ykpbMpCYtyZsmsWk3fPmkJ1sDyk5qppSi DCUpR3lKVvJGEYOwIiGsGMsqzrKKtVTELWEpS1r2Epe/5KUtfTnM2c1Rl8REJjCLCUv77TKXzwwm NJMZTWZOE5eIzKY2t8lN8Wzym+AMpzjHSc5xQkc8q0Gnk9L5G3b6xp3nbKc3q+hIejbSnozE5yL1 qRZIflqxno/0p0ADSlCAGvSeBUVoPQMCADs= ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0028.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0028_image179.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhywAVAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAABAC/ ABEAgwAAAHBub4KBgp+fn5KRkqurq7a2ttLS0sDAwNva2srJyfj4+Orq6uLi4vHx8f///wT/EMhJ q704682BOEsnfaHIMBYprpTKSif2PM2EzPSr7+Iyr77HKlYJ8jpGHdHyOFQGDcKREhBeqtPNb7Wd drMyK3jiWAgoqDGgIK6w1ZcvR76jwwH2KSEnSQzUAT5XgncUeWFgh2OKPAoPBmtObj4NZwABCTMM fx4ODzcADDhtoaN7M1E4Tg0zUgOiDpyIBw8OAjeoEqwPpwKeChMCCgsBDiguAASebKQSmJp/KrQL Us6ZD5sTM7h8uwS+n1bI4KAAC9RpFAMPAgK1Ep4CeyW2HlZ5XYFiDgkTxMmPALCSxSTgOgcADGCj 4CAAngd/MiGQEKRAAYRJBCzop7AZgHjz/8zNgLQHITx2ISX8UJjuo8N67ih2gWllHS8LokbBMLnl QT9DHh9ScDTxocMCkjJJWNeyQpctrApIKFB0izuTRoFKoGXJDgOe98R0+apSrJWoU6v+1KpyrTaP eQrkPMkAWFlEE6ogNACixgGpQu8+fGpWQkkYbxMLVkwo8AW5PQtTgCz5cCgy2Owu9lSXrVN2FXwx ODXhml18pDLJDUCLQAnHkQkrFviJgCTYr+VEnt3CGGnYYkT/dswKgW0KpnknX4zG5yV6YSnYjh6H VMljDzZOEMXJOV7H8xI4FOzutm4rnqS48+hJsOyTi7uEHz9hOu9ktEbMsDR5lKT9650BWq4xMglQ QxHsHHgSYGgZtpBC1VTHmygKiiRVAv8wFxhLANiE1Vvt7AcciAEyR6EhZxAYX4oIjUIHAj6sxYYt X0nxwEX1rIENfxOwwQCPb0xFygCcEfTZDzoJGeEltDRBHw5YJQmjGQscwKOQNDpwCpI4YClAjYOJ wcaSNzqQY5Jl5ljImmv6wuabGrgJ55x0boAAYHXSeWeefObpw2t9rvlnoIS+yQA1ha556JI8RAAA Ow== ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0028_image180.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhQAGdAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAwA0 AZkAgAAAAOrq6gL/jA+py93hopwA0oupzbz7D4biSCrbVqZVZKhi68byTNcXaof4suesDwwKhwzL CQJDomAro6mC5J2gCUR1WpwylU8jk2pKgonkshl0WK6gzrXSe+W239W6vf1siq30tft+h3c2SFj4 x4MYKJZ36LV4GGcnhUcpicUmaWm4yTnU4+cnCIo1V5RHmtl4uqqpmgjaGSurotYUBggZilsKGJXb OMer61o5a3yMlga3t+QYidCS5Ax9pJwVPcaFGRkm/fyFHC7+Mm5qXo6eTviJvMOuDh8vY1VOvy2P n6+/z9/v/w8woMCBBAsaPIgwocKFDBs6fAgxosSJFCtavIgxo8aN/xw7evwIMqTIkSRLmjyJMqXK lSxbunwJMyZKaGM8gHtAxt67MvYc7KTx84cUCTQf4GhGT1nQD0dSdVi6lIQooTrQOPVU49K5m5nA Xarl4ujVDFCDRIVl02qhs08ZvRqrZatbtJM4fHJGpSmiLctupgGD7YpRvLcC81mWLZrXanUVf+MW uDBianDy+pL8ps/lsdm6yE31rueEu8OwCdqzC6ctN1FOszJneJev2aDriEJVitRmwILjEtvNi9Jm pMRA/61NlCtVU6j0fNEtbNVxJ8db9QgeJ5jjuZi44lbTXGxq29nHS3/t+hWfYW5vxxB/ObdPz66y IN9tfX7xRO7ch/8SDwlq5uWni3/uRVYMWONl9hx/59DCSHy9dEVfdBSqkt4vC264moG/4DbhgLwF uN9U6TXXnogA5sIWWXxp5phfL/4lY2XU1ATZYjRqI42NfF1THTdsTKfYNODhdZhgQ26HXoxMltdN kEgdFuRAWp3UokxYcSZSlloKIVpJYX5JZplmnolmmmquyWabbr4JZ5xyzklnnXbeiWeeeu7JZ59+ /glooIIOSqibNCkXUFKxjMkdEF4exU5R9r01l1LkKOkllxhk2hZdDzKlFqdSZdXof8t5p5qmy2nY 6aU+RMVplqKmMCtV130aIVrAAWmXUEnq1Q2mrSV5T3neNDOYkJb/seajHsH29GJjPG73q33HdufN spBiWyR5lA6liTuwQIpoqsyd0tp9gZQIGzD1laZabO5Sh8t549pL77rbslbggrvul9dbRWHXbqWM mrsXK5lttYh2Hk6nCFgm+tvwjdxZ/Jk23r4Lb79DqrhxgpQytpquOuF6Q7vheUpwZ98+IiKBDnJs oodK5mpqyRau7C18tSEo870YWpMicmmFq/CHHCYtMIcSO0Vw1DT3nDGGTN9SH20Av0ai0EsbzZ6n m0ZL5bKTKkXYj3UBRm1/hC0ph49yYx0ltDOWjfeROTYLN47Cxa0xlNmdHCy3B89ypa0r1fpm4vqJ 3WWha4/NeESH/0uOeeaab855555/Dnrooo9Oeummn4566qqvznrrrr8Oe+yyz077CJLmcDkLO8Va +aqSA0vUqJBrQFZV37aasg29T7Si70fbXrxapUa/Ke4d5Q5XjQFf+w2SdByoV7fgIjhctqZZrJMc e1+DLd2UcYQ9XYk3FUyH9TYbGW8ty9ahz/zmO7GAdS1XAOQYRuJ3K6hFqGE1EdmAoFOvwuXLa+v5 A8QKJgz/ESlwy5OIWLTwCKlhSmbR8c3OUnOllsFMV0sT14mw1kEPoidVUlMhgZ62v+Kk8GsWYpcC 5WO/4ZkkfdeBkTWIVjZumU1IaPObpYr0HEds8GZR3BHbSOM+S//drHaGcNwMYshFInjxPWHkR/x0 AMYyqnGNbGyjG98IxzjKcY50rKMd74jHPOpxj3zsox//CMh6RPF5ZyTe8cYWSHElb2ZhoVUiEeY7 MNYqjZw7I/2ExSzLfO9YJDMcg3rjNkX0JouUpJMl9YXKjoVNQEGUkAlnJqHtUc10pyxQBR2otJA1 EF5SHFnHFlYu2VVCg1z7l85gyMurfDBp/VnjMFtIoX25MkTseeExLTjLMFKpbXHr28US4733jaKZ SmxfjwJZPZShcx8JXGc/vBNMd8pznvSspz3viU8yvuAnpbTnWYISqXyW4HxwQ+L5kKXKJmpLoDdI VxBtcSj1YDOglrTJH0PjhU1bsvKKtgGa/aZyUXyRCIgJo08yHxpSaRW0WzsilibZtqSXxjOlNK2p TW+K05zSUqfT46nyDpUUBlEnqATdIRSBCcNpCZCP9CqgfGqRLtP0y6EVXaUF8XPHaVBNO+PSqnMk GCL0vRKkdhRZAa2KM2NSNZcZ1Zoef/Wj9xHtYzF9UkvF17bHYAyQsxoj9XzKPqnMdDSDZV0BAAA7 ------=_NextPart_01C65BB3.9823E280 Content-Location: file:///C:/8C69C636/PhDcourse2006_bestanden/v3_slide0033.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="windows-1252" Slide 1
cDNA library