Figure 1. GFP vessels in the athymic Tie-2 GFP transgenic adult mouse.

A-G. Images from heart, kidney, liver, spleen, lung, brain and calf muscle respectively. Scale bar is 20 μm. H-J. Intravital fluorescence microscopy images of GFP vessels in the muscle (H-I) and in the ear (J)

Figure 2. Non-fluorescent vasculogenic mimicry channels contribute to in vivo hemodynamics.

A. Quantification of vessel diameter of different vascular structures in normal tissues and tumor. B. Blood vessel staining in a Ewing sarcoma xenograft. Non-endothelial cell–lined structures containing red blood cells, also referred to as blood lakes, are indicated by *. E-G. Intravital microscopy images of regular GFP expressing blood vessels and non-fluorescent vasculogenic mimicry channels. Different images recorded from the same vessels without filter (E), with I2 filter for GFP (F), and with N2.1 filter after injection of rhodamine (G).